1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT 51 CACAGTTAAA TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT 101 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG 151 CTTGGTTATG CCGGTACTGC CGGGCCTCTT GCGGGATATC CGGATATAGT 201 TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG 251 GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT 301 CGGGCTTTGT TAGCAGCCGG ATCCTCGAGC ATATGGCTGC CGCGCGGCAC 351 CAGGCCGCTG CTGTGATGAT GATGATGATG GCTGCTGCCC ATGGTATATC 401 TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT TGTTATCCGC 451 TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCTC 501 GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC GCCACAGGTG 551 CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT 601 CGCCACTTCG GGCTCATGAG CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG 651 CCCCGTGGCC GGGGGACTGT TGGGCGCCAT CTCCTTGCAT GCACCATTCC 701 TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG CTGCTTCCTA 751 ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GATCCCGGAC ACCATCGAAT 801 GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA 851 TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA 901 TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC 951 ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG 1001 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT 1051 GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA 1101 TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG 1151 GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA 1201 CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC TATCCGCTGG 1251 ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG 1301 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC 1351 CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC 1401 ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT 1451 CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC AAATTCAGCC 1501 GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT TTTCAACAAA 1551 CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT GCTGGTTGCC 1601 AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG AGTCCGGGCT 1651 GCGCGTTGGT GCGGATATCT CGGTAGTGGG ATACGACGAT ACCGAAGACA 1701 GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA TTTTCGCCTG 1751 CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC 1801 GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA 1851 CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 1901 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 1951 GCAACGCAAT TAATGTAAGT TAGCTCACTC ATTAGGCACC GGGATCTCGA 2001 CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG 2051 GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT ATCATGCAAC 2101 TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG CGAGGACCGC 2151 TTTCGCTGGA GCGCGACGAT GATCGGCCTG TCGCTTGCGG TATTCGGAAT 2201 CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC ACCAAACGTT 2251 TCGGCGAGAA GCAGGCCATT ATCGCCGGCA TGGCGGCCGA CGCGCTGGGC 2301 TACGTCTTGC TGGCGTTCGC GACGCGAGGC TGGATGGCCT TCCCCATTAT 2351 GATTCTTCTC GCTTCCGGCG GCATCGGGAT GCCCGCGTTG CAGGCCATGC 2401 TGTCCAGGCA GGTAGATGAC GACCATCAGG GACAGCTTCA AGGATCGCTC 2451 GCGGCTCTTA CCAGCCTAAC TTCGATCACT GGACCGCTGA TCGTCACGGC 2501 GATTTATGCC GCCTCGGCGA GCACATGGAA CGGGTTGGCA TGGATTGTAG 2551 GCGCCGCCCT ATACCTTGTC TGCCTCCCCG CGTTGCGTCG CGGTGCATGG 2601 AGCCGGGCCA CCTCGACCTG AATGGAAGCC GGCGGCACCT CGCTAACGGA 2651 TTCACCACTC CAAGAATTGG AGCCAATCAA TTCTTGCGGA GAACTGTGAA 2701 TGCGCAAACC AACCCTTGGC AGAACATATC CATCGCGTCC GCCATCTCCA 2751 GCAGCCGCAC GCGGCGCATC TCGGGCAGCG TTGGGTCCTG GCCACGGGTG 2801 CGCATGATCG TGCTCCTGTC GTTGAGGACC CGGCTAGGCT GGCGGGGTTG 2851 CCTTACTGGT TAGCAGAATG AATCACCGAT ACGCGAGCGA ACGTGAAGCG 2901 ACTGCTGCTG CAAAACGTCT GCGACCTGAG CAACAACATG AATGGTCTTC 2951 GGTTTCCGTG TTTCGTAAAG TCTGGAAACG CGGAAGTCAG CGCCCTGCAC 3001 CATTATGTTC CGGATCTGCA TCGCAGGATG CTGCTGGCTA CCCTGTGGAA 3051 CACCTACATC TGTATTAACG AAGCGCTGGC ATTGACCCTG AGTGATTTTT 3101 CTCTGGTCCC GCCGCATCCA TACCGCCAGT TGTTTACCCT CACAACGTTC 3151 CAGTAACCGG GCATGTTCAT CATCAGTAAC CCGTATCGTG AGCATCCTCT 3201 CTCGTTTCAT CGGTATCATT ACCCCCATGA ACAGAAATCC CCCTTACACG 3251 GAGGCATCAG TGACCAAACA GGAAAAAACC GCCCTTAACA TGGCCCGCTT 3301 TATCAGAAGC CAGACATTAA CGCTTCTGGA GAAACTCAAC GAGCTGGACG 3351 CGGATGAACA GGCAGACATC TGTGAATCGC TTCACGACCA CGCTGATGAG 3401 CTTTACCGCA GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG 3451 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 3501 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 3551 GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATACTGGCTT 3601 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATATGCGG 3651 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC 3701 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 3751 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 3801 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 3851 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 3901 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 3951 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 4001 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 4051 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 4101 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 4151 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 4201 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 4251 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 4301 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 4351 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 4401 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 4451 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 4501 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 4551 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 4601 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 4651 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 4701 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 4751 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 4801 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 4851 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 4901 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 4951 TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 5001 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 5051 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 5101 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 5151 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 5201 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 5251 CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 5301 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 5351 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 5401 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 5451 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 5501 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 5551 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 5601 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT 5651 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC 5701 TTCAAGAA D pET-15b "Times New RomanCD<T7_term"Times New RomanCDT7_Terminal_pri"Times New RomanCɆDT7_transl_en_RBS"Times New RomanDlacO"Times New RomanCDT7_pro"Times New RomanCD3F pBRrevBam_pri"Times New RomanCɆDYlacI"Times New RomanDORF 2"Times New RomanCDz ORF 3"Times New Roman*CD= ORF 3"Times New RomanCɆD=  tet(1191-576)"Times New RomanD { ROP"Times New RomanCDu  pGEX_3_pri"Times New RomanCD pBR322_origin"Times New RomanCɆD |ORF 2"Times New RomanD | Ampicillin"Times New RomanCDAmpR_pro"Times New Roman ClaI(24),HindIII(29)"Times New RomanDr' 2BamHI(319),XhoI(324),NdeI(331),NcoI(389),XbaI(428)"Times New Roman?D88g BglII"Times New RomanD>CHH BclI"Times New RomanDrpmft ApaI"Times New Roman D HpaI"Times New RomanD EagI(2284),NruI(2319)"Times New RomanDO`UhZpZp MscI"Times New Roman D PstI"Times New Roman^D  AatII(5635),EcoRI(5706)"Times New RomanDen test"Times New RomanD% v ] test"Times New Roman%D`^SRD=&=&