Sequence of pET-15b

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Sequence of pET-15b

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Sequence of pET-15b : 5708 BP; 1302 A; 1556 C; 1513 G; 1337 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... ..... BamHI(319),XhoI(324) ..... .......... ..........

301 cgggctttgt tagcagccgg atcctcgagc atatggctgc cgcgcggcac caggccgctg 360

301 gcccgaaaca atcgtcggcc taggagctcg tataccgacg gcgcgccgtg gtccggcgac 360

T7_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa 420

361 gacactacta ctactactac cgacgacggg taccatatag aggaagaatt tcaatttgtt 420

.......... .......... .......... ....... T7_transl_en_RBS .......

XbaI(428) .......... .......... .......... .......... ..........

421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa 480

421 ttaataaaga tctcccctta acaataggcg agtgttaagg ggatatcact cagcataatt 480

.......... .......... lacO .......... T7_pro ..........

.......... BglII(494) .......... .......... .......... ..........

481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc 540

481 aaagcgccct agctctagag ctaggagatg cggcctgcgt agcaccggcc gtagtggccg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct 600

541 cggtgtccac gccaacgacc gcggatatag cggctgtagt ggctacccct tctagcccga 600

.......... .......... ........ pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc 660

601 gcggtgaagc ccgagtactc gcgaacaaag ccgcacccat accaccgtcc ggggcaccgg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac 720

661 ccccctgaca acccgcggta gaggaacgta cgtggtaagg aacgccgccg ccacgagttg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga 780

721 ccggagttgg atgatgaccc gacgaaggat tacgtcctca gcgtattccc tctcgcagct 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga 840

781 ctagggcctg tggtagctta ccgcgttttg gaaagcgcca taccgtacta tcgcgggcct 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta 900

841 tctctcagtt aagtcccacc acttacactt tggtcattgc aatatgctac agcgtctcat 900

.......... .......... .......... lacI .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc 960

901 acggccacag agaatagtct ggcaaagggc gcaccacttg gtccggtcgg tgcaaagacg 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt 1020

961 cttttgcgcc ctttttcacc ttcgccgcta ccgcctcgac ttaatgtaag ggttggcgca 1020

.......... .......... lacI .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1021 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc 1080

1021 ccgtgttgtt gaccgcccgt ttgtcagcaa cgactaaccg caacggtgga ggtcagaccg 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc 1140

1081 ggacgtgcgc ggcagcgttt aacagcgccg ctaatttaga gcgcggctag ttgacccacg 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca 1200

1141 gtcgcaccac cacagctacc atcttgcttc gccgcagctt cggacatttc gccgccacgt 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... BclI(1230) .......... .......... ..........

1201 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga 1260

1201 gttagaagag cgcgttgcgc agtcacccga ctagtaattg ataggcgacc tactggtcct 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga 1320

1261 acggtaacga caccttcgac ggacgtgatt acaaggccgc aataaagaac tacagagact 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga 1380

1321 ggtctgtggg tagttgtcat aataaaagag ggtacttctg ccatgcgctg acccgcacct 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... ApaI(1427) ..........

1381 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt 1440

1381 cgtagaccag cgtaacccag tggtcgttta gcgcgacaat cgcccgggta attcaagaca 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc 1500

1441 gagccgcgca gacgcagacc gaccgaccgt atttatagag tgagcgttag tttaagtcgg 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat 1560

1501 ctatcgcctt gcccttccgc tgacctcacg gtacaggcca aaagttgttt ggtacgttta 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg 1620

1561 cgacttactc ccgtagcaag ggtgacgcta cgaccaacgg ttgctagtct accgcgaccc 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg 1680

1621 gcgttacgcg cggtaatggc tcaggcccga cgcgcaacca cgcctataga gccatcaccc 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... HpaI(1722) ..........

1681 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga 1740

1681 tatgctgcta tggcttctgt cgagtacaat atagggcggc aattggtggt agtttgtcct 1740

.......... .......... lacI ORF 2 .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1741 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc 1800

1741 aaaagcggac gaccccgttt ggtcgcacct ggcgaacgac gttgagagag tcccggtccg 1800

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc 1860

1801 ccacttcccg ttagtcgaca acgggcagag tgaccacttt tctttttggt gggaccgcgg 1860

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 1920

1861 gttatgcgtt tggcggagag gggcgcgcaa ccggctaagt aattacgtcg accgtgctgt 1920

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc 1980

1921 ccaaagggct gacctttcgc ccgtcactcg cgttgcgtta attacattca atcgagtgag 1980

.......... lacI ORF 2 ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc 2040

1981 taatccgtgg ccctagagct ggctacggga actctcggaa gttgggtcag tcgaggaagg 2040

.......... .......... ORF 3 ..... ORF 3 tet(1191-576) .....

.......... .......... .......... .......... .......... ..........

2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 2100

2041 ccacccgcgc cccgtactga tagcagcggc gtgaatactg acagaagaaa tagtacgttg 2100

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga 2160

2101 agcatcctgt ccacggccgt cgcgagaccc agtaaaagcc gctcctggcg aaagcgacct 2160

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag 2220

2161 cgcgctgcta ctagccggac agcgaacgcc ataagcctta gaacgtgcgg gagcgagttc 2220

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca 2280

2221 ggaagcagtg accagggcgg tggtttgcaa agccgctctt cgtccggtaa tagcggccgt 2280

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

EagI(2284) .......... .......... NruI(2319) .......... ..........

2281 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct 2340

2281 accgccggct gcgcgacccg atgcagaacg accgcaagcg ctgcgctccg acctaccgga 2340

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc 2400

2341 aggggtaata ctaagaagag cgaaggccgc cgtagcccta cgggcgcaac gtccggtacg 2400

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta 2460

2401 acaggtccgt ccatctactg ctggtagtcc ctgtcgaagt tcctagcgag cgccgagaat 2460

..... ORF 3 ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga 2520

2461 ggtcggattg aagctagtga cctggcgact agcagtgccg ctaaatacgg cggagccgct 2520

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg 2580

2521 cgtgtacctt gcccaaccgt acctaacatc cgcggcggga tatggaacag acggaggggc 2580

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct 2640

2581 gcaacgcagc gccacgtacc tcggcccggt ggagctggac ttaccttcgg ccgccgtgga 2640

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa 2700

2641 gcgattgcct aagtggtgag gttcttaacc tcggttagtt aagaacgcct cttgacactt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac 2760

2701 acgcgtttgg ttgggaaccg tcttgtatag gtagcgcagg cggtagaggt cgtcggcgtg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(2791) .......... ..........

2761 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc 2820

2761 cgccgcgtag agcccgtcgc aacccaggac cggtgcccac gcgtactagc acgaggacag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 2880

2821 caactcctgg gccgatccga ccgccccaac ggaatgacca atcgtcttac ttagtggcta 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 2940

2881 tgcgctcgct tgcacttcgc tgacgacgac gttttgcaga cgctggactc gttgttgtac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 3000

2941 ttaccagaag ccaaaggcac aaagcatttc agacctttgc gccttcagtc gcgggacgtg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 3060

3001 gtaatacaag gcctagacgt agcgtcctac gacgaccgat gggacacctt gtggatgtag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 3120

3061 acataattgc ttcgcgaccg taactgggac tcactaaaaa gagaccaggg cggcgtaggt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 3180

3121 atggcggtca acaaatggga gtgttgcaag gtcattggcc cgtacaagta gtagtcattg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 3240

3181 ggcatagcac tcgtaggaga gagcaaagta gccatagtaa tgggggtact tgtctttagg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 3300

3241 gggaatgtgc ctccgtagtc actggtttgt ccttttttgg cgggaattgt accgggcgaa 3300

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 3360

3301 atagtcttcg gtctgtaatt gcgaagacct ctttgagttg ctcgacctgc gcctacttgt 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 3420

3361 ccgtctgtag acacttagcg aagtgctggt gcgactactc gaaatggcgt cgacggagcg 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 3480

3421 cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct gccagtgtcg 3480

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 3540

3481 aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc gcccacaacc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 3600

3541 gcccacagcc ccgcgtcggt actgggtcag tgcatcgcta tcgcctcaca tatgaccgaa 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac 3660

3601 ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg tatatacgcc acactttatg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 3720

3661 gcgtgtctac gcattcctct tttatggcgt agtccgcgag aaggcgaagg agcgagtgac 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 3780

3721 tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt ttccgccatt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 3840

3781 atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt tttccggtcg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3900

3841 ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg 3900

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3960

3901 gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 4020

3961 tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 4080

4021 gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagtatcga 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 4140

4081 gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4200

4141 ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4260

4201 gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4320

4261 ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4380

4321 cctgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4440

4381 cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 4500

4441 tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 4560

4501 tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 4620

4561 agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4680

4621 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 4680

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4740

4681 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4800

4741 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4860

4801 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4920

4861 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... PstI(4958) .......... ..........

4921 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 4980

4921 gtcaattatc aaacgcgttg caacaacggt aacgacgtcc gtagcaccac agtgcgagca 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5040

4981 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5100

5041 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5160

5101 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5220

5161 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 5280

5221 catacgccgc tggctcaacg agaacgggcc gcagttgtgc cctattatgg cgcggtgtat 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5340

5281 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5400

5341 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 5460

5401 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5520

5461 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 5520

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 5580

5521 taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt acataaatct 5580

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 5640

5581 ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctgcagattc 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 5700

5641 tttggtaata atagtactgt aattggatat ttttatccgc atagtgctcc gggaaagcag 5700

.......... .......... .......... .......... .......... ..........

EcoRI(5706) ........ .......... .......... .......... ..........

5701 ttcaagaa.. .......... .......... .......... .......... .......... 5708

5701 aagttctt.. .......... .......... .......... .......... .......... 5708

.......... .......... .......... .......... .......... ..........