Sequence of pET-16b

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Sequence of pET-16b

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Sequence of pET-16b : 5711 BP; 1308 A; 1550 C; 1511 G; 1342 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... ..... BamHI(319),XhoI(324) ..... .......... ..........

301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg 360

301 gcccgaaaca atcgtcggcc taggagctcg tatactgctg gaagctatac cggcgacgac 360

T7_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa 420

361 actactacta ctactactac tactactacc gggtaccata tagaggaaga atttcaattt 420

... 6xHis 6xHis 6xHis 6xHis ... .......... ....... T7_transl_en_RBS

.......... XbaI(431) .......... .......... .......... ..........

421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat 480

421 gttttaataa agatctcccc ttaacaatag gcgagtgtta aggggatatc actcagcata 480

.......... .......... lacO .......... .......... T7_pro

.......... BglII(497) .......... .......... .......... ..........

481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 540

481 attaaagcgc cctagctcta gagctaggag atgcggcctg cgtagcaccg gccgtagtgg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 600

541 ccgcggtgtc cacgccaacg accgcggata tagcggctgt agtggctacc ccttctagcc 600

.......... .......... ........ pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 660

601 cgagcggtga agcccgagta ctcgcgaaca aagccgcacc cataccaccg tccggggcac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 720

661 cggccccctg acaacccgcg gtagaggaac gtacgtggta aggaacgccg ccgccacgag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 780

721 ttgccggagt tggatgatga cccgacgaag gattacgtcc tcagcgtatt ccctctcgca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc 840

781 gctctagggc ctgtggtagc ttaccgcgtt ttggaaagcg ccataccgta ctatcgcggg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga 900

841 ccttctctca gttaagtccc accacttaca ctttggtcat tgcaatatgc tacagcgtct 900

.......... .......... .......... lacI .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc 960

901 catacggcca cagagaatag tctggcaaag ggcgcaccac ttggtccggt cggtgcaaag 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca tt cccaaccg 1020

961 acgcttttgc gccctttttc accttcgccg ctaccgcctc gacttaatgt aagggttggc 1020

.......... .......... lacI .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1021 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct 1080

1021 gcaccgtgtt gttgaccgcc cgtttgtcag caacgactaa ccgcaacggt ggaggtcaga 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg 1140

1081 ccgggacgtg cgcggcagcg tttaacagcg ccgctaattt agagcgcggc tagttgaccc 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt 1200

1141 acggtcgcac caccacagct accatcttgc ttcgccgcag cttcggacat ttcgccgcca 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... BclI(1233) .......... ..........

1201 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca 1260

1201 cgtgttagaa gagcgcgttg cgcagtcacc cgactagtaa ttgataggcg acctactggt 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc 1320

1261 cctacggtaa cgacaccttc gacggacgtg attacaaggc cgcaataaag aactacagag 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt 1380

1321 actggtctgt gggtagttgt cataataaaa gagggtactt ctgccatgcg ctgacccgca 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... ApaI(1430) ..........

1381 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc 1440

1381 cctcgtagac cagcgtaacc cagtggtcgt ttagcgcgac aatcgcccgg gtaattcaag 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca 1500

1441 acagagccgc gcagacgcag accgaccgac cgtatttata gagtgagcgt tagtttaagt 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca 1560

1501 cggctatcgc cttgcccttc cgctgacctc acggtacagg ccaaaagttg tttggtacgt 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct 1620

1561 ttacgactta ctcccgtagc aagggtgacg ctacgaccaa cggttgctag tctaccgcga 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt 1680

1621 cccgcgttac gcgcggtaat ggctcaggcc cgacgcgcaa ccacgcctat agagccatca 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... HpaI(1725) ..........

1681 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca 1740

1681 ccctatgctg ctatggcttc tgtcgagtac aatatagggc ggcaattggt ggtagtttgt 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca 1800

1741 cctaaaagcg gacgaccccg tttggtcgca cctggcgaac gacgttgaga gagtcccggt 1800

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc 1860

1801 ccgccacttc ccgttagtcg acaacgggca gagtgaccac ttttcttttt ggtgggaccg 1860

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 1920

1861 cgggttatgc gtttggcgga gaggggcgcg caaccggcta agtaattacg tcgaccgtgc 1920

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca 1980

1921 tgtccaaagg gctgaccttt cgcccgtcac tcgcgttgcg ttaattacat tcaatcgagt 1980

.......... lacI ORF 2 ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 2040

1981 gagtaatccg tggccctaga gctggctacg ggaactctcg gaagttgggt cagtcgagga 2040

.......... .......... ORF 3 ..... ORF 3 tet(1191-576) .....

.......... .......... .......... .......... .......... ..........

2041 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 2100

2041 aggccacccg cgccccgtac tgatagcagc ggcgtgaata ctgacagaag aaatagtacg 2100

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 2160

2101 ttgagcatcc tgtccacggc cgtcgcgaga cccagtaaaa gccgctcctg gcgaaagcga 2160

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 2220

2161 cctcgcgctg ctactagccg gacagcgaac gccataagcc ttagaacgtg cgggagcgag 2220

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 2280

2221 ttcggaagca gtgaccaggg cggtggtttg caaagccgct cttcgtccgg taatagcggc 2280

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

EagI(2287) .......... .......... .......... NruI(2322) ..........

2281 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg 2340

2281 cgtaccgccg gctgcgcgac ccgatgcaga acgaccgcaa gcgctgcgct ccgacctacc 2340

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 2400

2341 ggaaggggta atactaagaa gagcgaaggc cgccgtagcc ctacgggcgc aacgtccggt 2400

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 2460

2401 acgacaggtc cgtccatcta ctgctggtag tccctgtcga agttcctagc gagcgccgag 2460

..... ORF 3 ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg 2520

2461 aatggtcgga ttgaagctag tgacctggcg actagcagtg ccgctaaata cggcggagcc 2520

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 2580

2521 gctcgtgtac cttgcccaac cgtacctaac atccgcggcg ggatatggaa cagacggagg 2580

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca 2640

2581 ggcgcaacgc agcgccacgt acctcggccc ggtggagctg gacttacctt cggccgccgt 2640

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt 2700

2641 ggagcgattg cctaagtggt gaggttctta acctcggtta gttaagaacg cctcttgaca 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg 2760

2701 cttacgcgtt tggttgggaa ccgtcttgta taggtagcgc aggcggtaga ggtcgtcggc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(2794) .......... ..........

2761 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct 2820

2761 gtgcgccgcg tagagcccgt cgcaacccag gaccggtgcc cacgcgtact agcacgagga 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 2880

2821 cagcaactcc tgggccgatc cgaccgcccc aacggaatga ccaatcgtct tacttagtgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 2940

2881 ctatgcgctc gcttgcactt cgctgacgac gacgttttgc agacgctgga ctcgttgttg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 3000

2941 tacttaccag aagccaaagg cacaaagcat ttcagacctt tgcgccttca gtcgcgggac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 3060

3001 gtggtaatac aaggcctaga cgtagcgtcc tacgacgacc gatgggacac cttgtggatg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 3120

3061 tagacataat tgcttcgcga ccgtaactgg gactcactaa aaagagacca gggcggcgta 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 3180

3121 ggtatggcgg tcaacaaatg ggagtgttgc aaggtcattg gcccgtacaa gtagtagtca 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 3240

3181 ttgggcatag cactcgtagg agagagcaaa gtagccatag taatgggggt acttgtcttt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 3300

3241 agggggaatg tgcctccgta gtcactggtt tgtccttttt tggcgggaat tgtaccgggc 3300

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

3301 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 3360

3301 gaaatagtct tcggtctgta attgcgaaga cctctttgag ttgctcgacc tgcgcctact 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 3420

3361 tgtccgtctg tagacactta gcgaagtgct ggtgcgacta ctcgaaatgg cgtcgacgga 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 3480

3421 gcgcgcaaag ccactactgc cacttttgga gactgtgtac gtcgagggcc tctgccagtg 3480

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 3540

3481 tcgaacagac attcgcctac ggccctcgtc tgttcgggca gtcccgcgca gtcgcccaca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 3600

3541 accgcccaca gccccgcgtc ggtactgggt cagtgcatcg ctatcgcctc acatatgacc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 3660

3601 gaattgatac gccgtagtct cgtctaacat gactctcacg tggtatatac gccacacttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 3720

3661 atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc gagaaggcga aggagcgagt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3780

3721 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3840

3781 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3900

3841 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 3900

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3960

3901 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4020

3961 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4080

4021 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4140

4081 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4200

4141 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4260

4201 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4320

4261 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 4380

4321 cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 4440

4381 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4500

4441 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 4560

4501 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtactct aatagttttt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 4620

4561 cctagaagtg gatctaggaa aatttaattt ttacttcaaa atttagttag atttcatata 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 4680

4621 tactcatttg aaccagactg tcaatggtta cgaattagtc actccgtgga tagagtcgct 4680

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 4740

4681 agacagataa agcaagtagg tatcaacgga ctgaggggca gcacatctat tgatgctatg 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 4800

4741 ccctcccgaa tggtagaccg gggtcacgac gttactatgg cgctctgggt gcgagtggcc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 4860

4801 gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 4920

4861 gttgaaatag gcggaggtag gtcagataat taacaacggc ccttcgatct cattcatcaa 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4961) ..........

4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 4980

4921 gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgacg tccgtagcac cacagtgcga 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5040

4981 gcagcaaacc ataccgaagt aagtcgaggc caagggttgc tagttccgct caatgtacta 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5100

5041 gggggtacaa cacgtttttt cgccaatcga ggaagccagg aggctagcaa cagtcttcat 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5160

5101 tcaaccggcg tcacaatagt gagtaccaat accgtcgtga cgtattaaga gaatgacagt 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5220

5161 acggtaggca ttctacgaaa agacactgac cactcatgag ttggttcagt aagactctta 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 5280

5221 tcacatacgc cgctggctca acgagaacgg gccgcagttg tgccctatta tggcgcggtg 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5340

5281 tatcgtcttg aaattttcac gagtagtaac cttttgcaag aagccccgct tttgagagtt 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 5400

5341 cctagaatgg cgacaactct aggtcaagct acattgggtg agcacgtggg ttgactagaa 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 5460

5401 gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt ttgtccttcc gttttacggc 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 5520

5461 gttttttccc ttattcccgc tgtgccttta caacttatga gtatgagaag gaaaaagtta 5520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 5580

5521 taataacttc gtaaatagtc ccaataacag agtactcgcc tatgtataaa cttacataaa 5580

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 5640

5581 tctttttatt tgtttatccc caaggcgcgt gtaaaggggc ttttcacggt ggactgcaga 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 5700

5641 ttctttggta ataatagtac tgtaattgga tatttttatc cgcatagtgc tccgggaaag 5700

.......... .......... .......... .......... .......... ..........

EcoRI(5709) ........ .......... .......... .......... ..........

5701 gtcttcaaga a......... .......... .......... .......... .......... 5711

5701 cagaagttct t......... .......... .......... .......... .......... 5711

.......... .......... .......... .......... .......... ..........