Sequence of pET-17b

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Sequence of pET-17b

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Sequence of pET-17b : 3306 BP; 818 A; 859 C; 815 G; 814 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc 60

1 aataggccta tatcaaggag gaaagtcgtt ttttggggag ttctgggcaa atctccgggg 60

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc 120

61 ttccccaata cgatcaataa cgagtcgcca ccgtcgtcgg ttgagtcgaa ggaaagcccg 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... ..... XhoI(141) NotI(147) ..... EcoRV(166) ..........

121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc 180

121 aaacaatcgt cggcctagac gagctcgccg gcggtcacac tacctataga cgtcttaagg 180

T7_term .......... .......... .......... .......... ..........

.......... SpeI(199) BamHI(205) SacI(215) .......... ..........

181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc 240

181 tcgtgtgacc gccggcaatg atcacctagg ctcgagccat ggttcgaact tagggctggg 240

.......... .......... .......... .......... .......... ..........

.......... ..... NheI(261) NdeI(268) ..... .......... ..........

241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa 300

241 taaacgacag gtggtcagta cgatcggtat acatatagag gaagaatttc aatttgtttt 300

T7_gene10_leader T7_leader Xpress_fwd_pri T7_transl_en_RBS ....

.......... .......... .......... .......... .......... ..........

301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga 360

301 aataaagatc tccctttggc aacaccagag ggatatcact cagcataatt aaagcgccct 360

.......... .......... .......... T7_pro .......... ..........

BglII(364) .......... MscI(389) .......... .......... ..........

361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt 420

361 agctctagag cccgtcgcaa cccaggaccg gtgcccacgc gtactagcac gaggacagca 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 480

421 actcctgggc cgatccgacc gccccaacgg aatgaccaat cgtcttactt agtggctatg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 540

481 cgctcgcttg cacttcgctg acgacgacgt tttgcagacg ctggactcgt tgttgtactt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca 600

541 accagaagcc aaaggcacaa agcatttcag acctttgcgc cttcagtcgc gggacgtggt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg 660

601 aatacaaggc ctagacgtag cgtcctacga cgaccgatgg gacaccttgt ggatgtagac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata 720

661 ataattgctt cgcgaccgta actgggactc actaaaaaga gaccagggcg gcgtaggtat 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc 780

721 ggcggtcaac aaatgggagt gttgcaaggt cattggcccg tacaagtagt agtcattggg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc 840

781 catagcactc gtaggagaga gcaaagtagc catagtaatg ggggtacttg tctttagggg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 900

841 gaatgtgcct ccgtagtcac tggtttgtcc ttttttggcg ggaattgtac cgggcgaaat 900

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 960

901 agtcttcggt ctgtaattgc gaagacctct ttgagttgct cgacctgcgc ctacttgtcc 960

.......... .......... ROP .......... .......... ..........

.......... .......... .......... ......... PvuII(1009) .........

961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 1020

961 gtctgtagac acttagcgaa gtgctggtgc gactactcga aatggcgtcg acggagcgcg 1020

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 1080

1021 caaagccact actgccactt ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa 1080

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 1140

1081 cagacattcg cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 1200

1141 ccacagcccc gcgtcggtac tgggtcagtg catcgctatc gcctcacata tgaccgaatt 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg 1260

1201 gatacgccgt agtctcgtct aacatgactc tcacgtggta tatacgccac actttatggc 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 1320

1261 gtgtctacgc attcctcttt tatggcgtag tccgcgagaa ggcgaaggag cgagtgactg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1380

1321 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1440

1381 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1500

1441 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 1500

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1560

1501 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1620

1561 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1680

1621 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1740

1681 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1800

1741 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1860

1801 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 1920

1861 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1980

1921 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2040

1981 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2100

2041 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2160

2101 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2220

2161 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2280

2221 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 2280

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2340

2281 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 2340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2400

2341 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 2400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2460

2401 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 2460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 2520

2461 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 2520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 2580

2521 caattatcaa acgcgttgca acaacggtaa cgacgtccgt agcaccacag tgcgagcagc 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2640

2581 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2700

2641 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2760

2701 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2820

2761 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 2880

2821 tacgccgctg gctcaacgag aacgggccgc agttgtgccc tattatggcg cggtgtatcg 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2940

2881 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 3000

2941 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 3060

3001 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3120

3061 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 3120

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3180

3121 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 3180

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... AatII(3233)

3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 3240

3181 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcagattctt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 3300

3241 tggtaataat agtactgtaa ttggatattt ttatccgcat agtgctccgg gaaagcagaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 caagaa.... .......... .......... .......... .......... .......... 3306

3301 gttctt.... .......... .......... .......... .......... .......... 3306

.......... .......... .......... .......... .......... ..........