xxLOCUS pET-17xb 4053 bp DNA SYN 30-Aug-2007 DEFINITION pET-17xb ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4053 /organism="pET-17xb" /mol_type="other DNA" terminator complement(3..131) /label="T7_terminator" misc_feature 71..89 /label="T7_Terminal_primer" CDS complement(72..1016) /label="ORF frame 3" /translation="MASMTGGQQMGTNQGKGVVAAGDKLALFLKVFGGEVLTAFARTS VTTSRHMVRSISSGKSAQFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTA DVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLG TATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAIL AALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTADSSLVPSSDPLVTAA SVLEFCRYPSHWRPLEQIRLLTKPERKLSWLLPPLSNN*" misc_feature complement(984..1016) /label="T7_gene10_leader" misc_feature complement(984..1016) /label="T7_leader" misc_feature complement(999..1017) /label="Xpress_fwd_primer" misc_feature complement(1025..1041) /label="T7_transl_en_RBS" promoter complement(1078..1096) /label="T7_promoter" misc_feature 1605..1796 /label="ROP" misc_feature complement(1790..1812) /label="pGEX_3_primer" rep_origin complement(2211..2830) /label="pBR322_origin" CDS complement(2985..3845) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2985..3845) /label="Ampicillin" /gene="Ampicillin" promoter complement(3887..3915) /label="AmpR_promoter" ORIGIN 1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc 61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc 121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc 181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atccgcggta 241 ccagcaccac cagcggtgag gtgcggaact tctacaacct caaagcccat aacgttgcgg 301 atagaaccct tctcagggtc aatcagagca gcgtagtttg ctgcgttcgg catcagtgct 361 gccagaatcg cagagtagct atctgggtca cagtagaaca cacggtcagc agccggaaca 421 tagttcttgg tcagagccgc acgagcctta gtcagagccg caataatctc cttacccagc 481 gcaacttggt cggtaagtgc ggccttgttc tgagtggtct caattacggt agcagtacct 541 aagccctcga tgttctcatt atatttgctt tccacgttac acagaccggc aatctcagcc 601 agaaccgcac catccgcagc catcgccaga gattcaccca actgagaggt atactcagag 661 cgaacgtcgt agtggttcat cgcgtcctca atatcataaa tcagaacgtc agccgtcagg 721 agaccgtcaa tggtgattac cttctcggtg tgtttgatgt ccttacgttt atcgtcgagg 781 ttctcgcccg gagccagata cgctgcctga gtgcgaccca gaacagggaa ctgagcggat 841 ttaccgctgg agatggaacg taccatgtgg cgagaagtgg tcacggaggt acgagcgaac 901 gcagtcagga cttcaccgcc aaataccttc aagaacaacg ccagtttatc tccagcagca 961 actacacctt taccttggtt agtacccatt tgctgtccac cagtcatgct agccatatgt 1021 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct 1081 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac 1141 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta 1201 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa 1261 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg 1321 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct 1381 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga 1441 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta 1501 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta 1561 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa 1621 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 1681 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 1741 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 1801 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 1861 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 1921 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 1981 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 2041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 2101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 2161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 2401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 2461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 2521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 2581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 2641 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 2701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 2761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 2821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 2881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 2941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 3001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 3121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 3181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3301 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 3361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 3421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 3481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 3541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 3601 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 3661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 3721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 3781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 3841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 3901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 3961 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 4021 aggcgtatca cgaggccctt tcgtcttcaa gaa // pET-17xb "Times New RomanxCT7_term"Times New RomanxCGYT7_Terminal_pri"Times New RomanxCɆHORF 3"Times New Roman*xT7_gene10_leader"Times New RomanxC T7_leader"Times New RomanxCXpress_fwd_pri"Times New Roman?xCɆT7_transl_en_RBS"Times New Romanx6HT7_pro"Times New RomanxCEROP"Times New RomanxC pGEX_3_pri"Times New RomanxCɆ  pBR322_origin"Times New Romanx ORF 3"Times New RomanxC  Ampicillin"Times New Roman(xC/KAmpR_pro"Times New Roman x`XhoI(141),NotI(147),EcoRV(166),EcoRI(174),SpeI(199),BamHI(205),SacI(215),HindIII(223),SacII(236)"Times New Roman #U- x7NheI(1008),NdeI(1015),XbaI(1053),BglII(1111),MscI(1136)"Times New Romanx xPvuII"Times New Roman  xAatII"Times New Roman__ xtest"Times New Roman wd xtest"Times New Romand