Sequence of pET-17xb

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Sequence of pET-17xb

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Sequence of pET-17xb : 4053 BP; 1005 A; 1065 C; 1002 G; 981 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc 60

1 aataggccta tatcaaggag gaaagtcgtt ttttggggag ttctgggcaa atctccgggg 60

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc 120

61 ttccccaata cgatcaataa cgagtcgcca ccgtcgtcgg ttgagtcgaa ggaaagcccg 120

....... T7_Terminal_pri T7_term ORF 3 ....... ..........

.......... ..... XhoI(141) NotI(147) ..... EcoRV(166) ..........

121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc 180

121 aaacaatcgt cggcctagac gagctcgccg gcggtcacac tacctataga cgtcttaagg 180

T7_term .......... ORF 3 .......... .......... ..........

......... SpeI BamHI(205) SacI(215) HindIII(223) SacII(236)

181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atccgcggta 240

181 tcgtgtgacc gccggcaatg atcacctagg ctcgagccat ggttcgaact taggcgccat 240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccagcaccac cagcggtgag gtgcggaact tctacaacct caaagcccat aacgttgcgg 300

241 ggtcgtggtg gtcgccactc cacgccttga agatgttgga gtttcgggta ttgcaacgcc 300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atagaaccct tctcagggtc aatcagagca gcgtagtttg ctgcgttcgg catcagtgct 360

301 tatcttggga agagtcccag ttagtctcgt cgcatcaaac gacgcaagcc gtagtcacga 360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gccagaatcg cagagtagct atctgggtca cagtagaaca cacggtcagc agccggaaca 420

361 cggtcttagc gtctcatcga tagacccagt gtcatcttgt gtgccagtcg tcggccttgt 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tagttcttgg tcagagccgc acgagcctta gtcagagccg caataatctc cttacccagc 480

421 atcaagaacc agtctcggcg tgctcggaat cagtctcggc gttattagag gaatgggtcg 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcaacttggt cggtaagtgc ggccttgttc tgagtggtct caattacggt agcagtacct 540

481 cgttgaacca gccattcacg ccggaacaag actcaccaga gttaatgcca tcgtcatgga 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aagccctcga tgttctcatt atatttgctt tccacgttac acagaccggc aatctcagcc 600

541 ttcgggagct acaagagtaa tataaacgaa aggtgcaatg tgtctggccg ttagagtcgg 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaaccgcac catccgcagc catcgccaga gattcaccca actgagaggt atactcagag 660

601 tcttggcgtg gtaggcgtcg gtagcggtct ctaagtgggt tgactctcca tatgagtctc 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgaacgtcgt agtggttcat cgcgtcctca atatcataaa tcagaacgtc agccgtcagg 720

661 gcttgcagca tcaccaagta gcgcaggagt tatagtattt agtcttgcag tcggcagtcc 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agaccgtcaa tggtgattac cttctcggtg tgtttgatgt ccttacgttt atcgtcgagg 780

721 tctggcagtt accactaatg gaagagccac acaaactaca ggaatgcaaa tagcagctcc 780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttctcgcccg gagccagata cgctgcctga gtgcgaccca gaacagggaa ctgagcggat 840

781 aagagcgggc ctcggtctat gcgacggact cacgctgggt cttgtccctt gactcgccta 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttaccgctgg agatggaacg taccatgtgg cgagaagtgg tcacggaggt acgagcgaac 900

841 aatggcgacc tctaccttgc atggtacacc gctcttcacc agtgcctcca tgctcgcttg 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gcagtcagga cttcaccgcc aaataccttc aagaacaacg ccagtttatc tccagcagca 960

901 cgtcagtcct gaagtggcgg tttatggaag ttcttgttgc ggtcaaatag aggtcgtcgt 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... NheI(1008) ..........

961 actacacctt taccttggtt agtacccatt tgctgtccac cagtcatgct agccatatgt 1020

961 tgatgtggaa atggaaccaa tcatgggtaa acgacaggtg gtcagtacga tcggtataca 1020

.......... ORF 3 T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

1021 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct 1080

1021 tatagaggaa gaatttcaat ttgttttaat aaagatctcc ctttggcaac accagaggga 1080

....... T7_transl_en_RBS ....... .......... .......... ..........

.......... .......... ......... BglII(1111) ......... MscI(1136)

1081 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac 1140

1081 tatcactcag cataattaaa gcgccctagc tctagagccc gtcgcaaccc aggaccggtg 1140

T7_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta 1200

1141 cccacgcgta ctagcacgag gacagcaact cctgggccga tccgaccgcc ccaacggaat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa 1260

1201 gaccaatcgt cttacttagt ggctatgcgc tcgcttgcac ttcgctgacg acgacgtttt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg 1320

1261 gcagacgctg gactcgttgt tgtacttacc agaagccaaa ggcacaaagc atttcagacc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct 1380

1321 tttgcgcctt cagtcgcggg acgtggtaat acaaggccta gacgtagcgt cctacgacga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga 1440

1381 ccgatgggac accttgtgga tgtagacata attgcttcgc gaccgtaact gggactcact 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta 1500

1441 aaaaagagac cagggcggcg taggtatggc ggtcaacaaa tgggagtgtt gcaaggtcat 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta 1560

1501 tggcccgtac aagtagtagt cattgggcat agcactcgta ggagagagca aagtagccat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa 1620

1561 agtaatgggg gtacttgtct ttagggggaa tgtgcctccg tagtcactgg tttgtccttt 1620

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

1621 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 1680

1621 tttggcggga attgtaccgg gcgaaatagt cttcggtctg taattgcgaa gacctctttg 1680

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 1740

1681 agttgctcga cctgcgccta cttgtccgtc tgtagacact tagcgaagtg ctggtgcgac 1740

.......... .......... ROP .......... .......... ..........

......... PvuII(1756) ......... .......... .......... ..........

1741 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 1800

1741 tactcgaaat ggcgtcgacg gagcgcgcaa agccactact gccacttttg gagactgtgt 1800

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

1801 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 1860

1801 acgtcgaggg cctctgccag tgtcgaacag acattcgcct acggccctcg tctgttcggg 1860

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 1920

1861 cagtcccgcg cagtcgccca caaccgccca cagccccgcg tcggtactgg gtcagtgcat 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 1980

1921 cgctatcgcc tcacatatga ccgaattgat acgccgtagt ctcgtctaac atgactctca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 2040

1981 cgtggtatat acgccacact ttatggcgtg tctacgcatt cctcttttat ggcgtagtcc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 2100

2041 gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga cgccgctcgc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 2160

2101 catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct attgcgtcct 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 2220

2161 ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg gcgcaacgac 2220

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 2280

2221 cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc gagttcagtc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 2340

2281 tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc ttcgagggag 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 2400

2341 cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa agagggaagc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 2460

2401 ccttcgcacc gcgaaagagt atcgagtgcg acatccatag agtcaagcca catccagcaa 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 2520

2461 gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 2580

2521 ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga ccgtcgtcgg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 2640

2581 tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa gaacttcacc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 2700

2641 accggattga tgccgatgtg atcttcctgt cataaaccat agacgcgaga cgacttcggt 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 2760

2701 caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg gcgaccatcg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 2820

2761 ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 2880

2821 ggaaactaga aaagatgccc cagactgcga gtcaccttgc ttttgagtgc aattccctaa 2880

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 2940

2881 aaccagtact ctaatagttt ttcctagaag tggatctagg aaaatttaat ttttacttca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 3000

2941 aaatttagtt agatttcata tatactcatt tgaaccagac tgtcaatggt tacgaattag 3000

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

3001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 3060

3001 tcactccgtg gatagagtcg ctagacagat aaagcaagta ggtatcaacg gactgagggg 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 3120

3061 cagcacatct attgatgcta tgccctcccg aatggtagac cggggtcacg acgttactat 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 3180

3121 ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 3240

3181 cggctcgcgt cttcaccagg acgttgaaat aggcggaggt aggtcagata attaacaacg 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 3300

3241 gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 3360

3301 cgtccgtagc accacagtgc gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 3420

3361 gctagttccg ctcaatgtac tagggggtac aacacgtttt ttcgccaatc gaggaagcca 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 3480

3421 ggaggctagc aacagtcttc attcaaccgg cgtcacaata gtgagtacca ataccgtcgt 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 3540

3481 gacgtattaa gagaatgaca gtacggtagg cattctacga aaagacactg accactcatg 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 3600

3541 agttggttca gtaagactct tatcacatac gccgctggct caacgagaac gggccgcagt 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 3660

3601 tgtgccctat tatggcgcgg tgtatcgtct tgaaattttc acgagtagta accttttgca 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 3720

3661 agaagccccg cttttgagag ttcctagaat ggcgacaact ctaggtcaag ctacattggg 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 3780

3721 tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 3840

3781 ttttgtcctt ccgttttacg gcgttttttc ccttattccc gctgtgcctt tacaacttat 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 3900

3841 gagtatgaga aggaaaaagt tataataact tcgtaaatag tcccaataac agagtactcg 3900

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 3960

3901 cctatgtata aacttacata aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg 3960

AmpR_pro .......... .......... .......... .......... ..........

......... AatII(3980) ......... .......... .......... ..........

3961 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 4020

3961 gcttttcacg gtggactgca gattctttgg taataatagt actgtaattg gatattttta 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aggcgtatca cgaggccctt tcgtcttcaa gaa....... .......... .......... 4053

4021 tccgcatagt gctccgggaa agcagaagtt ctt....... .......... .......... 4053

.......... .......... .......... .......... .......... ..........