Sequence of pET-19b

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Sequence of pET-19b

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Sequence of pET-19b : 5717 BP; 1306 A; 1551 C; 1514 G; 1346 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... ..... BamHI(319),XhoI(324) ..... .......... ..........

301 cgggctttgt tagcagccgg atcctcgagc atatgcttgt cgtcgtcgtc gatatggccg 360

301 gcccgaaaca atcgtcggcc taggagctcg tatacgaaca gcagcagcag ctataccggc 360

T7_term .......... .......... .......... EK ..........

.......... .......... .......... .......... .......... ..........

361 ctgctgtgat gatgatgatg atgatgatga tgatggccca tggtatatct ccttcttaaa 420

361 gacgacacta ctactactac tactactact actaccgggt accatataga ggaagaattt 420

........ 6xHis 6xHis 6xHis 6xHis ........ ....... T7_transl_en_RBS

.......... XbaI(437) .......... .......... .......... ..........

421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 480

421 caatttgttt taataaagat ctccccttaa caataggcga gtgttaaggg gatatcactc 480

.......... .......... .......... lacO .......... T7_pro

.......... .......... BglII(503) .......... .......... ..........

481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 540

481 agcataatta aagcgcccta gctctagagc taggagatgc ggcctgcgta gcaccggccg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 600

541 tagtggccgc ggtgtccacg ccaacgaccg cggatatagc ggctgtagtg gctacccctt 600

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 660

601 ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc cgcacccata ccaccgtccg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 720

661 gggcaccggc cccctgacaa cccgcggtag aggaacgtac gtggtaagga acgccgccgc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 780

721 cacgagttgc cggagttgga tgatgacccg acgaaggatt acgtcctcag cgtattccct 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 840

781 ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg aaagcgccat accgtactat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 900

841 cgcgggcctt ctctcagtta agtcccacca cttacacttt ggtcattgca atatgctaca 900

.......... .......... .......... .......... lacI ..........

.......... .......... .......... .......... .......... ..........

901 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 960

901 gcgtctcata cggccacaga gaatagtctg gcaaagggcg caccacttgg tccggtcggt 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga at tacattcc 1020

961 gcaaagacgc ttttgcgccc tttttcacct tcgccgctac cgcctcgact taatgtaagg 1020

.......... .......... lacI .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

1021 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 1080

1021 gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac gactaaccgc aacggtggag 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 1140

1081 gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc taatttagag cgcggctagt 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 1200

1141 tgacccacgg tcgcaccacc acagctacca tcttgcttcg ccgcagcttc ggacatttcg 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... BclI(1239) .......... ..........

1201 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 1260

1201 ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac tagtaattga taggcgacct 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 1320

1261 actggtccta cggtaacgac accttcgacg gacgtgatta caaggccgca ataaagaact 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 1380

1321 acagagactg gtctgtgggt agttgtcata ataaaagagg gtacttctgc catgcgctga 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(1436)

1381 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 1440

1381 cccgcacctc gtagaccagc gtaacccagt ggtcgtttag cgcgacaatc gcccgggtaa 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 1500

1441 ttcaagacag agccgcgcag acgcagaccg accgaccgta tttatagagt gagcgttagt 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 1560

1501 ttaagtcggc tatcgccttg cccttccgct gacctcacgg tacaggccaa aagttgtttg 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 1620

1561 gtacgtttac gacttactcc cgtagcaagg gtgacgctac gaccaacggt tgctagtcta 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 1680

1621 ccgcgacccg cgttacgcgc ggtaatggct caggcccgac gcgcaaccac gcctatagag 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(1731)

1681 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 1740

1681 ccatcaccct atgctgctat ggcttctgtc gagtacaata tagggcggca attggtggta 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 1800

1741 gtttgtccta aaagcggacg accccgtttg gtcgcacctg gcgaacgacg ttgagagagt 1800

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 1860

1801 cccggtccgc cacttcccgt tagtcgacaa cgggcagagt gaccactttt ctttttggtg 1860

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 1920

1861 ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta attacgtcga 1920

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 1980

1921 ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc gttgcgttaa ttacattcaa 1980

.......... lacI ORF 2 ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 2040

1981 tcgagtgagt aatccgtggc cctagagctg gctacgggaa ctctcggaag t tgggtcagt 2040

.......... .......... ORF 3 ..... ORF 3 tet(1191-576) .....

.......... .......... .......... .......... .......... ..........

2041 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 2100

2041 cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg tgaatactga cagaagaaat 2100

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 2160

2101 agtacgttga gcatcctgtc cacggccgtc gcgagaccca gtaaaagccg ctcctggcga 2160

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 2220

2161 aagcgacctc gcgctgctac tagccggaca gcgaacgcca taagccttag aacgtgcggg 2220

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 2280

2221 agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa gccgctcttc gtccggtaat 2280

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... EagI(2293) .......... .......... NruI(2328) ..........

2281 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 2340

2281 agcggccgta ccgccggctg cgcgacccga tgcagaacga ccgcaagcgc tgcgctccga 2340

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 2400

2341 cctaccggaa ggggtaatac taagaagagc gaaggccgcc gtagccctac gggcgcaacg 2400

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 2460

2401 tccggtacga caggtccgtc catctactgc tggtagtccc tgtcgaagtt cctagcgagc 2460

..... ORF 3 ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 2520

2461 gccgagaatg gtcggattga agctagtgac ctggcgacta gcagtgccgc taaatacggc 2520

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 2580

2521 ggagccgctc gtgtaccttg cccaaccgta cctaacatcc gcggcgggat atggaacaga 2580

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 2640

2581 cggaggggcg caacgcagcg ccacgtacct cggcccggtg gagctggact taccttcggc 2640

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 2700

2641 cgccgtggag cgattgccta agtggtgagg ttcttaacct cggttagtta agaacgcctc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 2760

2701 ttgacactta cgcgtttggt tgggaaccgt cttgtatagg tagcgcaggc ggtagaggtc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(2800) .......... ..........

2761 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 2820

2761 gtcggcgtgc gccgcgtaga gcccgtcgca acccaggacc ggtgcccacg cgtactagca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 2880

2821 cgaggacagc aactcctggg ccgatccgac cgccccaacg gaatgaccaa tcgtcttact 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 2940

2881 tagtggctat gcgctcgctt gcacttcgct gacgacgacg ttttgcagac gctggactcg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 3000

2941 ttgttgtact taccagaagc caaaggcaca aagcatttca gacctttgcg ccttcagtcg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 3060

3001 cgggacgtgg taatacaagg cctagacgta gcgtcctacg acgaccgatg ggacaccttg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg 3120

3061 tggatgtaga cataattgct tcgcgaccgt aactgggact cactaaaaag agaccagggc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc 3180

3121 ggcgtaggta tggcggtcaa caaatgggag tgttgcaagg tcattggccc gtacaagtag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa 3240

3181 tagtcattgg gcatagcact cgtaggagag agcaaagtag ccatagtaat gggggtactt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat 3300

3241 gtctttaggg ggaatgtgcc tccgtagtca ctggtttgtc cttttttggc gggaattgta 3300

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

3301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc 3360

3301 ccgggcgaaa tagtcttcgg tctgtaattg cgaagacctc tttgagttgc tcgacctgcg 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag 3420

3361 cctacttgtc cgtctgtaga cacttagcga agtgctggtg cgactactcg aaatggcgtc 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 3480

3421 gacggagcgc gcaaagccac tactgccact tttggagact gtgtacgtcg agggcctctg 3480

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

3481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 3540

3481 ccagtgtcga acagacattc gcctacggcc ctcgtctgtt cgggcagtcc cgcgcagtcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 3600

3541 cccacaaccg cccacagccc cgcgtcggta ctgggtcagt gcatcgctat cgcctcacat 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt 3660

3601 atgaccgaat tgatacgccg tagtctcgtc taacatgact ctcacgtggt atatacgcca 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 3720

3661 cactttatgg cgtgtctacg cattcctctt ttatggcgta gtccgcgaga aggcgaagga 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3780

3721 gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3840

3781 tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3900

3841 ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg 3900

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3960

3901 aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4020

3961 gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4080

4021 gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4140

4081 gagtatcgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4200

4141 cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4260

4201 tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4320

4261 cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4380

4321 tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4440

4381 ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4500

4441 cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4560

4501 gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4620

4561 gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4680

4621 catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga 4680

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4740

4681 gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcac atctattgat 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4800

4741 gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4860

4801 gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4920

4861 caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4967) ..........

4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 4980

4921 catcaagcgg tcaattatca aacgcgttgc aacaacggta acgacgtccg tagcaccaca 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5040

4981 gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5100

5041 gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5160

5101 cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5220

5161 gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 5280

5221 ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttgtgcc ctattatggc 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5340

5281 gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5400

5341 agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5460

5401 ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5520

5461 tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa 5520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5580

5521 aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta 5580

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5640

5581 cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5700

5641 tgcagattct ttggtaataa tagtactgta attggatatt tttatccgca tagtgctccg 5700

.......... .......... .......... .......... .......... ..........

......... EcoRI(5715) ......... .......... .......... ..........

5701 cctttcgtct tcaagaa... .......... .......... .......... .......... 5717

5701 ggaaagcaga agttctt... .......... .......... .......... .......... 5717

.......... .......... .......... .......... .......... ..........