Sequence of pET-20b(+)

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Sequence of pET-20b(+)

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Sequence of pET-20b(+) : 3716 BP; 933 A; 946 C; 946 G; 891 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 240

181 gctgcctcga gcttaagcct aggcttaatt aaggctatag gtaccggtag cggccgaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(288) ..........

241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 300

241 gtcgctcctc gtcgtctggt cgtcgtcgcc agccgtcgtc cataaagtat acatatagag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc cctatagtga 360

301 gaagaatttc aatttgtttt aataaagatc tccctttggc aacaccagag ggatatcact 360

T7_transl_en_RBS .... .......... .......... .......... T7_pro

.......... .......... BglII(384) .......... .......... ..........

361 gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg 420

361 cagcataatt aaagcgccct agctctagag cccgtcgcaa cccaggaccg gtgcccacgc 420

T7_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 480

421 gtactagcac gaggacagca actcctgggc cgatccgacc gccccaacgg aatgaccaat 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 540

481 cgtcttactt agtggctatg cgctcgcttg cacttcgctg acgacgacgt tttgcagacg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 600

541 ctggactcgt tgttgtactt accagaagcc aaaggcacaa agcatttcag acctttgcgc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 660

601 cttcagtcgc gggacgtggt aatacaaggc ctagacgtag cgtcctacga cgaccgatgg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 720

661 gacaccttgt ggatgtagac ataattgctt cgcgaccgta actgggactc actaaaaaga 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 780

721 gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt cattggcccg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 840

781 tacaagtagt agtcattggg catagcactc gtaggagaga gcaaagtagc catagtaatg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 900

841 ggggtacttg tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc ttttttggcg 900

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

901 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 960

901 ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc gaagacctct ttgagttgct 960

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 1020

961 cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc gactactcga 1020

.......... .......... ROP .......... .......... ..........

PvuII(1029) ........ .......... .......... .......... ..........

1021 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 1080

1021 aatggcgtcg acggagcgcg caaagccact actgccactt ttggagactg tgtacgtcga 1080

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

1081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 1140

1081 gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 1200

1141 gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg catcgctatc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 1260

1201 gcctcacata tgaccgaatt gatacgccgt agtctcgtct aacatgactc tcacgtggta 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 1320

1261 tatacgccac actttatggc gtgtctacgc attcctcttt tatggcgtag tccgcgagaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 1380

1321 ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 1440

1381 gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 1500

1441 acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 1560

1501 aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 1620

1561 ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 1680

1621 gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 1740

1681 accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 1800

1741 tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 1860

1801 tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 1920

1861 tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 1980

1921 tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 2040

1981 agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 2100

2041 aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 2160

2101 agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 2220

2161 actctaatag tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 2280

2221 gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc 2280

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 2340

2281 gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 2400

2341 tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc 2400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 2460

2401 tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 2520

2461 cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... PstI(2576)

2521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 2580

2521 gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa cgacgtccgt 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 2640

2581 agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt 2640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 2700

2641 ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 2760

2701 agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 2820

2761 taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 2880

2821 tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 2940

2881 tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 3000

2941 ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 3060

3001 gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 3120

3061 cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 3180

3121 agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt 3180

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 3240

3181 ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 3300

3241 acggtggact ttaacatttg caattataaa acaattttaa gcgcaattta aaaacaattt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 3360

3301 agtcgagtaa aaaattggtt atccggcttt agccgtttta gggaatattt agttttctta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 3420

3361 tctggctcta tcccaactca caacaaggtc aaaccttgtt ctcaggtgat aatttcttgc 3420

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 3480

3421 acctgaggtt gcagtttccc gctttttggc agatagtccc gctaccgggt gatgcacttg 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 3540

3481 gtagtgggat tagttcaaaa aaccccagct ccacggcatt tcgtgattta gccttgggat 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 3600

3541 ttccctcggg ggctaaatct cgaactgccc ctttcggccg cttgcaccgc tctttccttc 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 3660

3601 ccttctttcg ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag tgcgacgcgc 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca.... 3716

3661 attggtggtg tgggcggcgc gaattacgcg gcgatgtccc gcgcagggta agcggt.... 3716

.......... f1_origin .......... .......... .......... ..........