\\(%LOCUS pET-21(+) 5369 bp DNA SYN 27-Aug-2007 DEFINITION pET-21(+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5369 /organism="pET-21(+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(208..235) /label="lacO" promoter complement(235..253) /label="T7_promoter" misc_feature complement(337..356) /label="pBRrevBam_primer" gene complement(285..548) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 631..1722 /label="lacI" CDS 763..1722 /label="ORF frame 1" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(1781..2056) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2531..2722 /label="ROP" misc_feature complement(2716..2738) /label="pGEX_3_primer" rep_origin complement(3137..3756) /label="pBR322_origin" CDS complement(3911..4771) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3911..4771) /label="Ampicillin" /gene="Ampicillin" promoter complement(4813..4841) /label="AmpR_promoter" rep_origin complement(5035..5341) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tcctagaggg gaattgttat ccgctcacaa ttcccctata 241 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg 301 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 361 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 421 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg 481 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 541 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca 601 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac 661 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 721 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 781 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 841 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 901 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 961 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 1021 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 1081 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 1141 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 1201 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 1261 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 1321 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 1381 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 1441 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 1501 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1561 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 1621 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 1681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 1741 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc 1801 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 1861 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga 1921 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca 1981 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc 2041 cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac 2101 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg 2161 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt 2221 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt 2281 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac 2341 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag 2401 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt 2461 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac 2521 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag 2581 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat 2641 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg 2701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 2761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 2821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 2881 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat 2941 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc 3001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 3061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 3121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 3421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 3481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 3541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 3601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 3661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 3721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 3781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 3961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 4021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 4081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 4141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4201 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta 4261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 4441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 4501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 4561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 4681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 4741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 4801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 4861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata 4921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 4981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 5041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 5161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 5221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5341 cgccgctaca gggcgcgtcc cattcgcca // pET-21(+)"Times New Roman\CT7_term"Times New Roman\CEWT7_Terminal_pri"Times New Roman\CɆ6xHis"Times New Roman\lacO"Times New Roman\CT7_pro"Times New Roman\CɆ$ tet(564-300)"Times New Roman\CQd pBRrevBam_pri"Times New Roman\wlacI"Times New Roman\CORF 1"Times New Roman\C tet(852-576)"Times New Roman\CɆ ROP"Times New Roman\  pGEX_3_pri"Times New Roman\CA  pBR322_origin"Times New Roman\CGORF 2"Times New Roman\CɆG Ampicillin"Times New Roman(\AmpR_pro"Times New Roman\C f1_origin"Times New Roman \BclI"Times New Romanmu~~ \ApaI"Times New Roman |N]PAP \PstI"Times New RomanBHFi?M \test"Times New Roman*~ \test"Times New Roman    \UXhoI(159),NotI(167),HindIII(174),SalI(180),SacI(191),EcoRI(193),BamHI(199),BglII(269) Arial{aa \HpaI(1497),PvuII(1591) Arial|