Sequence of pET-21(+)

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pET-21(+)

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pET-21(+): 5369 BP; 1267 A; 1427 C; 1432 G; 1243 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... ......... XhoI(159) NotI(167) HindIII(174)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

..... SacI(191),EcoRI(193) ..... .......... .......... ..........

181 cgacggagct cgaattcgga tcctagaggg gaattgttat ccgctcacaa ttcccctata 240

181 gctgcctcga gcttaagcct aggatctccc cttaacaata ggcgagtgtt aaggggatat 240

.......... .......... .......... .......... lacO ..........

.......... .......... BglII(269) .......... .......... ..........

241 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg 300

241 cactcagcat aattaaagcg ccctagctct agagctagga gatgcggcct gcgtagcacc 300

T7_pro .......... .......... .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

301 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 360

301 ggccgtagtg gccgcggtgt ccacgccaac gaccgcggat atagcggctg ta gtggctac 360

.......... ......... tet(564-300) ....... pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

361 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 420

361 cccttctagc ccgagcggtg aagcccgagt actcgcgaac aaagccgcac ccataccacc 420

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg 480

421 gtccggggca ccggccccct gacaacccgc ggtagaggaa cgtacgtggt aaggaacgcc 480

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 540

481 gccgccacga gttgccggag ttggatgatg acccgacgaa ggattacgtc ctcagcgtat 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca 600

541 tccctctcgc agctctaggg cctgtggtag cttaccgcgt tttggaaagc gccataccgt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac 660

601 actatcgcgg gccttctctc agttaagtcc caccacttac actttggtca ttgcaatatg 660

.......... .......... .......... .......... lacI ..........

.......... .......... .......... .......... .......... ..........

661 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 720

661 ctacagcgtc tcatacggcc acagagaata gtctggcaaa gggcgcacca cttggtccgg 720

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 780

721 tcggtgcaaa gacgcttttg cgcccttttt caccttcgcc gctaccgcct cgacttaatg 780

.......... .......... lacI .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

781 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 840

781 taagggttgg cgcaccgtgt tgttgaccgc ccgtttgtca gcaacgacta accgcaacgg 840

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 900

841 tggaggtcag accgggacgt gcgcggcagc gtttaacagc gccgctaatt tagagcgcgg 900

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 960

901 ctagttgacc cacggtcgca ccaccacagc taccatcttg cttcgccgca gcttcggaca 960

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... BclI(1004) ..........

961 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 1020

961 tttcgccgcc acgtgttaga agagcgcgtt gcgcagtcac ccgactagta attgataggc 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 1080

1021 gacctactgg tcctacggta acgacacctt cgacggacgt gattacaagg ccgcaataaa 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 1140

1081 gaactacaga gactggtctg tgggtagttg tcataataaa agagggtact tctgccatgc 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 1200

1141 gctgacccgc acctcgtaga ccagcgtaac ccagtggtcg tttagcgcga caatcgcccg 1200

.......... .......... lacI ORF 1 .......... .......... ..........

ApaI(1201) .......... .......... .......... .......... ..........

1201 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 1260

1201 ggtaattcaa gacagagccg cgcagacgca gaccgaccga ccgtatttat agagtgagcg 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 1320

1261 ttagtttaag tcggctatcg ccttgccctt ccgctgacct cacggtacag gccaaaagtt 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 1380

1321 gtttggtacg tttacgactt actcccgtag caagggtgac gctacgacca acggttgcta 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 1440

1381 gtctaccgcg acccgcgtta cgcgcggtaa tggctcaggc ccgacgcgca accacgccta 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 1500

1441 tagagccatc accctatgct gctatggctt ctgtcgagta caatataggg cggcaattgg 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1560

1501 tggtagtttg tcctaaaagc ggacgacccc gtttggtcgc acctggcgaa cgacgttgag 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... ......... PvuII(1591) ......... ..........

1561 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 1620

1561 agagtcccgg tccgccactt cccgttagtc gacaacgggc agagtgacca cttttctttt 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 1680

1621 tggtgggacc gcgggttatg cgtttggcgg agaggggcgc gcaaccggct aagtaattac 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 1740

1681 gtcgaccgtg ctgtccaaag ggctgacctt tcgcccgtca ctcgcgttgc gttaattaca 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc 1800

1741 ttcaatcgag tgagtaatcc gtggccctag agctggctac gggaactctc ggaagttggg 1800

.......... .......... .......... ......... tet(852-576) .........

.......... .......... .......... .......... .......... ..........

1801 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 1860

1801 tcagtcgagg aaggccaccc gcgccccgta ctgatagcag cggcgtgaat actgacagaa 1860

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga 1920

1861 gaaatagtac gttgagcatc ctgtccacgg ccgtcgcgag acccagtaaa agccgctcct 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca 1980

1921 ggcgaaagcg acctcgcgct gctactagcc ggacagcgaa cgccataagc cttagaacgt 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc 2040

1981 gcgggagcga gttcggaagc agtgaccagg gcggtggttt gcaaagccgc tcttcgtccg 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac 2100

2041 gtaatagcgg ccgtaccgcc ggggtgccca cgcgtactag cacgaggaca gcaactcctg 2100

tet(852-576) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg 2160

2101 ggccgatccg accgccccaa cggaatgacc aatcgtctta cttagtggct atgcgctcgc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt 2220

2161 ttgcacttcg ctgacgacga cgttttgcag acgctggact cgttgttgta cttaccagaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt 2280

2221 gccaaaggca caaagcattt cagacctttg cgccttcagt cgcgggacgt ggtaatacaa 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac 2340

2281 ggcctagacg tagcgtccta cgacgaccga tgggacacct tgtggatgta gacataattg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag 2400

2341 cttcgcgacc gtaactggga ctcactaaaa agagaccagg gcggcgtagg tatggcggtc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt 2460

2401 aacaaatggg agtgttgcaa ggtcattggc ccgtacaagt agtagtcatt gggcatagca 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac 2520

2461 ctcgtaggag agagcaaagt agccatagta atgggggtac ttgtctttag ggggaatgtg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag 2580

2521 cctccgtagt cactggtttg tccttttttg gcgggaattg taccgggcga aatagtcttc 2580

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat 2640

2581 ggtctgtaat tgcgaagacc tctttgagtt gctcgacctg cgcctacttg tccgtctgta 2640

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg 2700

2641 gacacttagc gaagtgctgg tgcgactact cgaaatggcg tcgacggagc gcgcaaagcc 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 2760

2701 actactgcca cttttggaga ctgtgtacgt cgagggcctc tgccagtgtc gaacagacat 2760

.......... ROP pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 2820

2761 tcgcctacgg ccctcgtctg ttcgggcagt cccgcgcagt cgcccacaac cgcccacagc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 2880

2821 cccgcgtcgg tactgggtca gtgcatcgct atcgcctcac atatgaccga attgatacgc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat 2940

2881 cgtagtctcg tctaacatga ctctcacgtg gtatatacgc cacactttat ggcgtgtcta 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc 3000

2941 cgcattcctc ttttatggcg tagtccgcga gaaggcgaag gagcgagtga ctgagcgacg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 3060

3001 cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 3120

3061 ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3180

3121 ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg 3180

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3240

3181 tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3300

3241 tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3360

3301 ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg agtgcgacat 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 3420

3361 ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 3480

3421 aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 3540

3481 tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc tccatacatc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 3600

3541 cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcctgtcata 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 3660

3601 aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 3720

3661 ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 3780

3721 cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca 3780

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3840

3781 ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc tagaagtgga 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3900

3841 tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata ctcatttgaa 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 3960

3901 ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag acagataaag 3960

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 4020

3961 caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc ctcccgaatg 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 4080

4021 gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga ggtctaaata 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 4140

4081 gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt tgaaataggc 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4200

4141 ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc ggtcaattat 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... PstI(4229) .......... .......... ..........

4201 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta 4260

4201 caaacgcgtt gcaacaacgg taacgacgtc cgtagcacca cagtgcgagc agcaaaccat 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4320

4261 accgaagtaa gtcgaggcca agggttgcta gttccgctca atgtactagg gggtacaaca 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4380

4321 cgttttttcg ccaatcgagg aagccaggag gctagcaaca gtcttcattc aaccggcgtc 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 4440

4381 acaatagtga gtaccaatac cgtcgtgacg tattaagaga atgacagtac ggtaggcatt 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 4500

4441 ctacgaaaag acactgacca ctcatgagtt ggttcagtaa gactcttatc acatacgccg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 4560

4501 ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4620

4561 attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 4680

4621 acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 4740

4681 gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 4800

4741 attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt 4800

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 4860

4801 aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc tttttatttg 4860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata 4920

4861 tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actttaacat ttgcaattat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 4980

4921 aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg gttatccggc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 5040

4981 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5100

5041 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 5160

5101 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca aaaaacccca 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 5220

5161 gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa tctcgaactg 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5280

5221 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5340

5281 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgccgctaca gggcgcgtcc cattcgcca. .......... .......... .......... 5369

5341 gcggcgatgt cccgcgcagg gtaagcggt. .......... .......... .......... 5369

.......... .......... .......... .......... .......... ..........