Sequence of pET-21_a

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Sequence of pET-21_a_(+)

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Sequence of pET-21 a (+) : 5443 BP; 1288 A; 1446 C; 1441 G; 1268 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... NheI(231)

181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata 240

181 gctgcctcga gcttaagcct aggcgctggg taaacgacag gtggtcagta cgatcggtat 240

.......... .......... .......... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... XbaI(276) .......... ..........

241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc 300

241 acatatagag gaagaatttc aatttgtttt aataaagatc tccccttaac aataggcgag 300

....... T7_transl_en_RBS ....... .......... .......... lacO

.......... .......... .......... .......... BglII(342) ..........

301 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc 360

301 tgttaagggg atatcactca gcataattaa agcgccctag ctctagagct aggagatgcg 360

lacO T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 420

361 gcctgcgtag caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg 420

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

421 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 480

421 gctgtagtgg ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc 480

pBRrevBam_pri ..... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc 540

481 gcacccatac caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaacgtacg 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 600

541 tggtaaggaa cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct 660

601 cgtcctcagc gtattccctc tcgcagctct agggcctgtg gtagcttacc gcgttttgga 660

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac 720

661 aagcgccata ccgtactatc gcgggccttc tctcagttaa gtcccaccac ttacactttg 720

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

721 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 780

721 gtcattgcaa tatgctacag cgtctcatac ggccacagag aatagtctgg caaagggcgc 780

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 840

781 accacttggt ccggtcggtg caaagacgct tttgcgccct ttttcacctt cgccgctacc 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 900

841 gcctcgactt aatgtaaggg ttggcgcacc gtgttgttga ccgcccgttt gtcagcaacg 900

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 960

901 actaaccgca acggtggagg tcagaccggg acgtgcgcgg cagcgtttaa cagcgccgct 960

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 1020

961 aatttagagc gcggctagtt gacccacggt cgcaccacca cagctaccat cttgcttcgc 1020

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1078)

1021 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 1080

1021 cgcagcttcg gacatttcgc cgccacgtgt tagaagagcg cgttgcgcag tcacccgact 1080

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 1140

1081 agtaattgat aggcgaccta ctggtcctac ggtaacgaca ccttcgacgg acgtgattac 1140

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 1200

1141 aaggccgcaa taaagaacta cagagactgg tctgtgggta gttgtcataa taaaagaggg 1200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 1260

1201 tacttctgcc atgcgctgac ccgcacctcg tagaccagcg taacccagtg gtcgtttagc 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... ApaI(1275) .......... .......... .......... ..........

1261 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 1320

1261 gcgacaatcg cccgggtaat tcaagacaga gccgcgcaga cgcagaccga ccgaccgtat 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 1380

1321 ttatagagtg agcgttagtt taagtcggct atcgccttgc ccttccgctg acctcacggt 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 1440

1381 acaggccaaa agttgtttgg tacgtttacg acttactccc gtagcaaggg tgacgctacg 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 1500

1441 accaacggtt gctagtctac cgcgacccgc gttacgcgcg gtaatggctc aggcccgacg 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 1560

1501 cgcaaccacg cctatagagc catcacccta tgctgctatg gcttctgtcg agtacaatat 1560

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(1570) .......... .......... .......... .......... ..........

1561 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 1620

1561 agggcggcaa ttggtggtag tttgtcctaa aagcggacga ccccgtttgg tcgcacctgg 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 1680

1621 cgaacgacgt tgagagagtc ccggtccgcc acttcccgtt agtcgacaac gggcagagtg 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 1740

1681 accacttttc tttttggtgg gaccgcgggt tatgcgtttg gcggagaggg gcgcgcaacc 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 1800

1741 ggctaagtaa ttacgtcgac cgtgctgtcc aaagggctga cctttcgccc gtcactcgcg 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg 1860

1801 ttgcgttaat tacattcaat cgagtgagta atccgtggcc ctagagctgg ctacgggaac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 1920

1861 tctcggaagt tgggtcagtc gaggaaggcc acccgcgccc cgtactgata gcagcggcgt 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc 1980

1921 gaatactgac agaagaaata gtacgttgag catcctgtcc acggccgtcg cgagacccag 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta 2040

1981 taaaagccgc tcctggcgaa agcgacctcg cgctgctact agccggacag cgaacgccat 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc 2100

2041 aagccttaga acgtgcggga gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc 2160

2101 ccgctcttcg tccggtaata gcggccgtac cgccggggtg cccacgcgta ctagcacgag 2160

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca 2220

2161 gacagcaact cctgggccga tccgaccgcc ccaacggaat gaccaatcgt cttacttagt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca 2280

2221 ggctatgcgc tcgcttgcac ttcgctgacg acgacgtttt gcagacgctg gactcgttgt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc 2340

2281 tgtacttacc agaagccaaa ggcacaaagc atttcagacc tttgcgcctt cagtcgcggg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct 2400

2341 acgtggtaat acaaggccta gacgtagcgt cctacgacga ccgatgggac accttgtgga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc 2460

2401 tgtagacata attgcttcgc gaccgtaact gggactcact aaaaagagac cagggcggcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca 2520

2461 taggtatggc ggtcaacaaa tgggagtgtt gcaaggtcat tggcccgtac aagtagtagt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga 2580

2521 cattgggcat agcactcgta ggagagagca aagtagccat agtaatgggg gtacttgtct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc 2640

2581 ttagggggaa tgtgcctccg tagtcactgg tttgtccttt tttggcggga attgtaccgg 2640

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2641 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat 2700

2641 gcgaaatagt cttcggtctg taattgcgaa gacctctttg agttgctcga cctgcgccta 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc 2760

2701 cttgtccgtc tgtagacact tagcgaagtg ctggtgcgac tactcgaaat ggcgtcgacg 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 2820

2761 gagcgcgcaa agccactact gccacttttg gagactgtgt acgtcgaggg cctctgccag 2820

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2821 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 2880

2821 tgtcgaacag acattcgcct acggccctcg tctgttcggg cagtcccgcg cagtcgccca 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact 2940

2881 caaccgccca cagccccgcg tcggtactgg gtcagtgcat cgctatcgcc tcacatatga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga 3000

2941 ccgaattgat acgccgtagt ctcgtctaac atgactctca cgtggtatat acgccacact 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 3060

3001 ttatggcgtg tctacgcatt cctcttttat ggcgtagtcc gcgagaaggc gaaggagcga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3120

3061 gtgactgagc gacgcgagcc agcaagccga cgccgctcgc catagtcgag tgagtttccg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3180

3121 ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3240

3181 ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3300

3241 ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3360

3301 tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3420

3361 ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3480

3421 atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3540

3481 cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3600

3541 gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3660

3601 tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3720

3661 atcttcctgt cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3780

3721 accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3840

3781 cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 3900

3841 cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact ctaatagttt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 3960

3901 ttcctagaag tggatctagg aaaatttaat ttttacttca aaatttagtt agatttcata 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4020

3961 tatactcatt tgaaccagac tgtcaatggt tacgaattag tcactccgtg gatagagtcg 4020

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 4080

4021 ctagacagat aaagcaagta ggtatcaacg gactgagggg cagcacatct attgatgcta 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4140

4081 tgccctcccg aatggtagac cggggtcacg acgttactat ggcgctctgg gtgcgagtgg 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4200

4141 ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc cggctcgcgt cttcaccagg 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4260

4201 acgttgaaat aggcggaggt aggtcagata attaacaacg gcccttcgat ctcattcatc 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4303) ..........

4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 4320

4261 aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga cgtccgtagc accacagtgc 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4380

4321 gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt gctagttccg ctcaatgtac 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4440

4381 tagggggtac aacacgtttt ttcgccaatc gaggaagcca ggaggctagc aacagtcttc 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4500

4441 attcaaccgg cgtcacaata gtgagtacca ataccgtcgt gacgtattaa gagaatgaca 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 4560

4501 gtacggtagg cattctacga aaagacactg accactcatg agttggttca gtaagactct 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 4620

4561 tatcacatac gccgctggct caacgagaac gggccgcagt tatgccctat tatggcgcgg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 4680

4621 tgtatcgtct tgaaattttc acgagtagta accttttgca agaagccccg cttttgagag 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 4740

4681 ttcctagaat ggcgacaact ctaggtcaag ctacattggg tgagcacgtg ggttgactag 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 4800

4741 aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 4860

4801 gcgttttttc ccttattccc gctgtgcctt tacaacttat gagtatgaga aggaaaaagt 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 4920

4861 tataataact tcgtaaatag tcccaataac agagtactcg cctatgtata aacttacata 4920

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4921 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat 4980

4921 aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg gtggacttta 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 5040

4981 acatttgcaa ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 5100

5041 attggttatc cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 5160

5101 caactcacaa caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca 5160

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 5220

5161 gtttcccgct ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 5280

5221 ttcaaaaaac cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 5340

5281 taaatctcga actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 5400

5341 tcctcgcccg cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca....... .......... 5443

5401 gcggcgcgaa ttacgcggcg atgtcccgcg cagggtaagc ggt....... .......... 5443

f1_origin .......... .......... .......... .......... ..........