Sequence of pET-21_b

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Sequence of pET-21_b_(+)

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Sequence of pET-21 b (+) : 5442 BP; 1288 A; 1446 C; 1440 G; 1268 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ NheI(230) ..........

181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 240

181 gctgcctcga gcttaagcct agggctgggt aaacgacagg tggtcagtac gatcggtata 240

.......... .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... XbaI(275) .......... ..........

241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 300

241 catatagagg aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt 300

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... .......... BglII(341) ..........

301 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 360

301 gttaagggga tatcactcag cataattaaa gcgccctagc tctagagcta ggagatgcgg 360

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 420

361 cctgcgtagc accggccgta gtggccgcgg tgtccacgcc aacgaccgcg gatatagcgg 420

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 480

421 ctgtagtggc taccccttct agcccgagcg gtgaagcccg agtactcgcg aacaaagccg 480

pBRrevBam_pri ..... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 540

481 cacccatacc accgtccggg gcaccggccc cctgacaacc cgcggtagag gaacgtacgt 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 600

541 ggtaaggaac gccgccgcca cgagttgccg gagttggatg atgacccgac gaaggattac 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 660

601 gtcctcagcg tattccctct cgcagctcta gggcctgtgg tagcttaccg cgttttggaa 660

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 720

661 agcgccatac cgtactatcg cgggccttct ctcagttaag tcccaccact tacactttgg 720

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

721 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 780

721 tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc aaagggcgca 780

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 840

781 ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc gccgctaccg 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 900

841 cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg tcagcaacga 900

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 960

901 ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac agcgccgcta 960

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1020

961 atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc ttgcttcgcc 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1077)

1021 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1080

1021 gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt cacccgacta 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1140

1081 gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga cgtgattaca 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1200

1141 aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat aaaagagggt 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1260

1201 acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg tcgtttagcg 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1274) .......... .......... .......... ..........

1261 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1320

1261 cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac cgaccgtatt 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1380

1321 tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga cctcacggta 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1440

1381 caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt gacgctacga 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1500

1441 ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca ggcccgacgc 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1560

1501 gcaaccacgc ctatagagcc atcaccctat gctgctatgg cttctgtcga gtacaatata 1560

.......... .......... lacI ORF 2 .......... .......... ..........

HpaI(1569) .......... .......... .......... .......... ..........

1561 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1620

1561 gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt cgcacctggc 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1680

1621 gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg ggcagagtga 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1740

1681 ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1800

1741 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1860

1801 tgcgttaatt acattcaatc gagtgagtaa tccgtggccc tagagctggc tacgggaact 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 1920

1861 ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 1980

1921 aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagacccagt 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2040

1981 aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc gaacgccata 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2100

2041 agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg tttgcaaagc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc 2160

2101 cgctcttcgt ccggtaatag cggccgtacc gccggggtgc ccacgcgtac tagcacgagg 2160

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac 2220

2161 acagcaactc ctgggccgat ccgaccgccc caacggaatg accaatcgtc ttacttagtg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa 2280

2221 gctatgcgct cgcttgcact tcgctgacga cgacgttttg cagacgctgg actcgttgtt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct 2340

2281 gtacttacca gaagccaaag gcacaaagca tttcagacct ttgcgccttc agtcgcggga 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta 2400

2341 cgtggtaata caaggcctag acgtagcgtc ctacgacgac cgatgggaca ccttgtggat 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca 2460

2401 gtagacataa ttgcttcgcg accgtaactg ggactcacta aaaagagacc agggcggcgt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag 2520

2461 aggtatggcg gtcaacaaat gggagtgttg caaggtcatt ggcccgtaca agtagtagtc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa 2580

2521 attgggcata gcactcgtag gagagagcaa agtagccata gtaatggggg tacttgtctt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc 2640

2581 tagggggaat gtgcctccgt agtcactggt ttgtcctttt ttggcgggaa ttgtaccggg 2640

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2641 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg 2700

2641 cgaaatagtc ttcggtctgt aattgcgaag acctctttga gttgctcgac ctgcgcctac 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc 2760

2701 ttgtccgtct gtagacactt agcgaagtgc tggtgcgact actcgaaatg gcgtcgacgg 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 2820

2761 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 2820

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 2880

2821 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 2940

2881 aaccgcccac agccccgcgt cggtactggg tcagtgcatc gctatcgcct cacatatgac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa 3000

2941 cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtggtatata cgccacactt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 3060

3001 tatggcgtgt ctacgcattc ctcttttatg gcgtagtccg cgagaaggcg aaggagcgag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3120

3061 tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3180

3121 cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3240

3181 gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3300

3241 gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3360

3301 gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3420

3361 gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaagagta 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3480

3421 tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga cccgacacac 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3540

3481 gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc agaactcagg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3600

3541 ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3660

3601 cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3720

3661 tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3780

3721 ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3840

3781 gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3900

3841 agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc taatagtttt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3960

3901 tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta gatttcatat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4020

3961 atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg atagagtcgc 4020

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4021 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4080

4021 tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta ttgatgctat 4080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4140

4081 gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg tgcgagtggc 4140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4200

4141 cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc ttcaccagga 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4260

4201 cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc tcattcatca 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4302) ..........

4261 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 4320

4261 agcggtcaat tatcaaacgc gttgcaacaa cggtaacgac gtccgtagca ccacagtgcg 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4380

4321 agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc tcaatgtact 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4440

4381 agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca acagtcttca 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4500

4441 ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag agaatgacag 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4560

4501 tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag taagactctt 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4620

4561 atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt atggcgcggt 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4680

4621 gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 4740

4681 tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg gttgactaga 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 4800

4741 agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 4860

4801 cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 4920

4861 ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa acttacataa 4920

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 4980

4921 atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg tggactttaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 5040

4981 catttgcaat tataaaacaa ttttaagcgc aatttaaaaa caatttagtc gagtaaaaaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 5100

5041 ttggttatcc ggctttagcc gttttaggga atatttagtt ttcttatctg gctctatccc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 5160

5101 aactcacaac aaggtcaaac cttgttctca ggtgataatt tcttgcacct gaggttgcag 5160

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 5220

5161 tttcccgctt tttggcagat agtcccgcta ccgggtgatg cacttggtag tgggattagt 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 5280

5221 tcaaaaaacc ccagctccac ggcatttcgt gatttagcct tgggatttcc ctcgggggct 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 5340

5281 aaatctcgaa ctgccccttt cggccgcttg caccgctctt tccttccctt ctttcgcttt 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 5400

5341 cctcgcccgc gatcccgcga ccgttcacat cgccagtgcg acgcgcattg gtggtgtggg 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca........ .......... 5442

5401 cggcgcgaat tacgcggcga tgtcccgcgc agggtaagcg gt........ .......... 5442

f1_origin .......... .......... .......... .......... ..........