&LOCUS pET-21 c (+) 5441 bp DNA SYN 05-Sep-2007 DEFINITION pET-21 c (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5441 /organism="pET-21 c (+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(205..237) /label="T7_gene10_leader" misc_feature complement(205..237) /label="T7_leader" misc_feature complement(220..238) /label="Xpress_fwd_primer" misc_feature complement(246..262) /label="T7_transl_en_RBS" misc_feature complement(280..307) /label="lacO" promoter complement(307..325) /label="T7_promoter" misc_feature complement(409..428) /label="pBRrevBam_primer" gene complement(357..620) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 703..1794 /label="lacI" CDS 835..1794 /label="ORF frame 1" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(1853..2128) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2603..2794 /label="ROP" misc_feature complement(2788..2810) /label="pGEX_3_primer" rep_origin complement(3209..3828) /label="pBR322_origin" CDS complement(3983..4843) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3983..4843) /label="Ampicillin" /gene="Ampicillin" promoter complement(4885..4913) /label="AmpR_promoter" rep_origin complement(5107..5413) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 3901 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 3961 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 4021 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 4081 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 4141 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 4201 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 4261 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 4321 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 4381 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 4441 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 4501 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 4561 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 4621 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 4681 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 4741 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 4801 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 4861 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4921 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg 4981 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 5041 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 5101 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 5161 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 5221 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 5281 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 5341 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 5401 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a //ApET-21 c (+) "Times New RomanCAT7_term"Times New RomanCAEWT7_Terminal_pri"Times New RomanCɆA6xHis"Times New RomanAT7_gene10_leader"Times New RomanCA T7_leader"Times New RomanCAXpress_fwd_pri"Times New Roman*CɆAT7_transl_en_RBS"Times New RomanA3lacO"Times New RomanCA3ET7_pro"Times New RomanCɆAel tet(564-300)"Times New RomanCA pBRrevBam_pri"Times New RomanAlacI"Times New RomanCACORF 1"Times New RomanCA=P tet(852-576)"Times New RomanCɆA+ ROP"Times New RomanA  pGEX_3_pri"Times New RomanCA  pBR322_origin"Times New RomanCAORF 2"Times New RomanCɆA Ampicillin"Times New Roman(A1AmpR_pro"Times New RomanCA% f1_origin"Times New Roman rXhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),NheI(229),NdeI(236),XbaI(274)"Times New Roman A|R  BglII"Times New RomanTAAA BclI"Times New Roman4A}XTPIW ApaI"Times New RomanA EcoRV(1512),HpaI(1568)"Times New RomanA PstI"Times New RomanAkhev^l test"Times New RomanA@A >  test"Times New RomanA#((