Sequence of pET-21_d

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Sequence of pET-21_d_(+)

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Sequence of pET-21 d (+) : 5440 BP; 1287 A; 1445 C; 1441 G; 1267 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt 240

181 gctgcctcga gcttaagcct aggctgggta aacgacaggt ggtcagtacg atcggtacca 240

.......... .......... .......... .. T7_gene10_leader T7_leader ..

.......... .......... .......... XbaI(273) .......... ..........

241 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 300

241 tatagaggaa gaatttcaat ttgttttaat aaagatctcc ccttaacaat aggcgagtgt 300

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... BglII(339) .......... ..........

301 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg 360

301 taaggggata tcactcagca taattaaagc gccctagctc tagagctagg agatgcggcc 360

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 420

361 tgcgtagcac cggccgtagt ggccgcggtg tccacgccaa cgaccgcgga tatagcggct 420

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 480

421 gtagtggcta ccccttctag cccgagcggt gaagcccgag tactcgcgaa caaagccgca 480

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 540

481 cccataccac cgtccggggc accggccccc tgacaacccg cggtagagga acgtacgtgg 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 600

541 taaggaacgc cgccgccacg agttgccgga gttggatgat gacccgacga aggattacgt 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc 660

601 cctcagcgta ttccctctcg cagctctagg gcctgtggta gcttaccgcg ttttggaaag 660

tet(564-300) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 720

661 cgccataccg tactatcgcg ggccttctct cagttaagtc ccaccactta cactttggtc 720

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

721 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 780

721 attgcaatat gctacagcgt ctcatacggc cacagagaat agtctggcaa agggcgcacc 780

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 840

781 acttggtccg gtcggtgcaa agacgctttt gcgccctttt tcaccttcgc cgctaccgcc 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 900

841 tcgacttaat gtaagggttg gcgcaccgtg ttgttgaccg cccgtttgtc agcaacgact 900

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 960

901 aaccgcaacg gtggaggtca gaccgggacg tgcgcggcag cgtttaacag cgccgctaat 960

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 1020

961 ttagagcgcg gctagttgac ccacggtcgc accaccacag ctaccatctt gcttcgccgc 1020

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1075)

1021 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 1080

1021 agcttcggac atttcgccgc cacgtgttag aagagcgcgt tgcgcagtca cccgactagt 1080

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 1140

1081 aattgatagg cgacctactg gtcctacggt aacgacacct tcgacggacg tgattacaag 1140

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 1200

1141 gccgcaataa agaactacag agactggtct gtgggtagtt gtcataataa aagagggtac 1200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 1260

1201 ttctgccatg cgctgacccg cacctcgtag accagcgtaa cccagtggtc gtttagcgcg 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... ApaI(1272) .......... .......... .......... ..........

1261 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 1320

1261 acaatcgccc gggtaattca agacagagcc gcgcagacgc agaccgaccg accgtattta 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 1380

1321 tagagtgagc gttagtttaa gtcggctatc gccttgccct tccgctgacc tcacggtaca 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 1440

1381 ggccaaaagt tgtttggtac gtttacgact tactcccgta gcaagggtga cgctacgacc 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 1500

1441 aacggttgct agtctaccgc gacccgcgtt acgcgcggta atggctcagg cccgacgcgc 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 1560

1501 aaccacgcct atagagccat caccctatgc tgctatggct tctgtcgagt acaatatagg 1560

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(1567) .......... .......... .......... .......... ..........

1561 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 1620

1561 gcggcaattg gtggtagttt gtcctaaaag cggacgaccc cgtttggtcg cacctggcga 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 1680

1621 acgacgttga gagagtcccg gtccgccact tcccgttagt cgacaacggg cagagtgacc 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 1740

1681 acttttcttt ttggtgggac cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 1800

1741 taagtaatta cgtcgaccgt gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga 1860

1801 cgttaattac attcaatcga gtgagtaatc cgtggcccta gagctggcta cgggaactct 1860

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

1861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 1920

1861 cggaagttgg gtcagtcgag gaaggccacc cgcgccccgt actgatagca gcggcgtgaa 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 1980

1921 tactgacaga agaaatagta cgttgagcat cctgtccacg gccgtcgcga gacccagtaa 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 2040

1981 aagccgctcc tggcgaaagc gacctcgcgc tgctactagc cggacagcga acgccataag 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 2100

2041 ccttagaacg tgcgggagcg agttcggaag cagtgaccag ggcggtggtt tgcaaagccg 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg 2160

2101 ctcttcgtcc ggtaatagcg gccgtaccgc cggggtgccc acgcgtacta gcacgaggac 2160

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 2220

2161 agcaactcct gggccgatcc gaccgcccca acggaatgac caatcgtctt acttagtggc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 2280

2221 tatgcgctcg cttgcacttc gctgacgacg acgttttgca gacgctggac tcgttgttgt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 2340

2281 acttaccaga agccaaaggc acaaagcatt tcagaccttt gcgccttcag tcgcgggacg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca 2400

2341 tggtaataca aggcctagac gtagcgtcct acgacgaccg atgggacacc ttgtggatgt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 2460

2401 agacataatt gcttcgcgac cgtaactggg actcactaaa aagagaccag ggcggcgtag 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 2520

2461 gtatggcggt caacaaatgg gagtgttgca aggtcattgg cccgtacaag tagtagtcat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 2580

2521 tgggcatagc actcgtagga gagagcaaag tagccatagt aatgggggta cttgtcttta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 2640

2581 gggggaatgt gcctccgtag tcactggttt gtcctttttt ggcgggaatt gtaccgggcg 2640

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2641 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 2700

2641 aaatagtctt cggtctgtaa ttgcgaagac ctctttgagt tgctcgacct gcgcctactt 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc 2760

2701 gtccgtctgt agacacttag cgaagtgctg gtgcgactac tcgaaatggc gtcgacggag 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2820

2761 cgcgcaaagc cactactgcc acttttggag actgtgtacg tcgagggcct ctgccagtgt 2820

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2880

2821 cgaacagaca ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 2940

2881 ccgcccacag ccccgcgtcg gtactgggtc agtgcatcgc tatcgcctca catatgaccg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat 3000

2941 aattgatacg ccgtagtctc gtctaacatg actctcacgt ggtatatacg ccacacttta 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 3060

3001 tggcgtgtct acgcattcct cttttatggc gtagtccgcg agaaggcgaa ggagcgagtg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3120

3061 actgagcgac gcgagccagc aagccgacgc cgctcgccat agtcgagtga gtttccgcca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3180

3121 ttatgccaat aggtgtctta gtcccctatt gcgtcctttc ttgtacactc gttttccggt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 3240

3181 cgttttccgg tccttggcat ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3300

3241 gggactgctc gtagtgtttt tagctgcgag ttcagtctcc accgctttgg gctgtcctga 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3360

3301 tatttctatg gtccgcaaag ggggaccttc gagggagcac gcgagaggac aaggctggga 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3420

3361 cggcgaatgg cctatggaca ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3480

3421 gagtgcgaca tccatagagt caagccacat ccagcaagcg aggttcgacc cgacacacgt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3540

3481 gcttgggggg caagtcgggc tggcgacgcg gaataggcca ttgatagcag aactcaggtt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3600

3541 gggccattct gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3660

3601 ctccatacat ccgccacgat gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3720

3661 ttcctgtcat aaaccataga cgcgagacga cttcggtcaa tggaagcctt tttctcaacc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3780

3721 atcgagaact aggccgtttg tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 3840

3781 cgtctaatgc gcgtcttttt ttcctagagt tcttctagga aactagaaaa gatgccccag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 3900

3841 actgcgagtc accttgcttt tgagtgcaat tccctaaaac cagtactcta atagtttttc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 3960

3901 ctagaagtgg atctaggaaa atttaatttt tacttcaaaa tttagttaga tttcatatat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4020

3961 actcatttga accagactgt caatggttac gaattagtca ctccgtggat agagtcgcta 4020

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4080

4021 gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt gatgctatgc 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4140

4081 cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg cgagtggccg 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4200

4141 aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4260

4201 ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc attcatcaag 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... PstI(4300) .......... ..........

4261 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 4320

4261 cggtcaatta tcaaacgcgt tgcaacaacg gtaacgacgt ccgtagcacc acagtgcgag 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4380

4321 cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4440

4381 ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4500

4441 caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4560

4501 cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 4620

4561 cacatacgcc gctggctcaa cgagaacggg ccgcagttat gccctattat ggcgcggtgt 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 4680

4621 atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 4740

4681 ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 4800

4741 tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 4860

4801 ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 4920

4861 aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat 4920

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 4980

4921 ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactttaaca 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 5040

4981 tttgcaatta taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 5100

5041 ggttatccgg ctttagccgt tttagggaat atttagtttt cttatctggc tctatcccaa 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 5160

5101 ctcacaacaa ggtcaaacct tgttctcagg tgataatttc ttgcacctga ggttgcagtt 5160

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 5220

5161 tcccgctttt tggcagatag tcccgctacc gggtgatgca cttggtagtg ggattagttc 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 5280

5221 aaaaaacccc agctccacgg catttcgtga tttagccttg ggatttccct cgggggctaa 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 5340

5281 atctcgaact gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 5400

5341 tcgcccgcga tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca .......... .......... 5440

5401 gcgcgaatta cgcggcgatg tcccgcgcag ggtaagcggt .......... .......... 5440

f1_origin .......... .......... .......... .......... ..........