3W;pET-21a(+)-AroQ "Times New RomanWC;T7_term"Times New RomanWC;EWT7_Terminal_pri"Times New RomanWCɆ;6xHis"Times New RomanW;T7_transl_en_RBS"Times New RomanWC; lacO"Times New RomanWC; T7_pro"Times New RomanW;>E tet(564-300)"Times New RomanWCɆ;r pBRrevBam_pri"Times New RomanWC;lacI"Times New Roman(WC;ORF 3"Times New RomanWCɆ; )  tet(852-576)"Times New RomanW; ROP"Times New RomanWC;  pGEX_3_pri"Times New RomanWC;b pBR322_origin"Times New RomanWCɆ;hORF 1"Times New RomanW;h Ampicillin"Times New RomanWC; AmpR_pro"Times New RomanWC; f1_origin"Times New Roman WOXhoI(158), NotI(166), EagI(166), HindIII(173), SalI(179), SacI(190), BamHI(198)"Times New Roman;*[ WNheI"Times New Roman ;.7?"\ WNdeI"Times New Roman ;bovY WXbaI(747), BglII(813)"Times New Roman- ;s WApaI"Times New Roman; WEcoRV(1985), HpaI(2041)"Times New Roman; WPstI"Times New Roman ;F@92@ Wtest"Times New Roman|;} rggi Wtest"Times New Roman;c)