Sequence of pET-21aPlus

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Sequence of pET-21aPlus

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Sequence of pET-21a(+)-AroQ : 5914 BP; 1411 A; 1544 C; 1530 G; 1429 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190) BamHI(198) .......... .......... .......... ..........

181 cgacggagct cgaattcgga tccttatttt tgattgttaa tggggttatt agggttgttt 240

181 gctgcctcga gcttaagcct aggaataaaa actaacaatt accccaataa tcccaacaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttttgggctt cttggaacgc tttcatttcg gctaaaatat tgaccatcgc cattaaagcc 300

241 aaaacccgaa gaaccttgcg aaagtaaagc cgattttata actggtagcg gtaatttcgg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atgttgtagc caagcgggcc aaatcccatg atcacgcctc cacaagccgc tccagtgtaa 360

301 tacaacatcg gttcgcccgg tttagggtac tagtgcggag gtgttcggcg aggtcacatt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gaatttttcc tgaattcctc tctggcttga atgttagtga gatgcacttc aataacgggt 420

361 cttaaaaagg acttaaggag agaccgaact tacaatcact ctacgtgaag ttattgccca 420

.......... .......... .......... .......... .......... ..........

NheI(427) .......... .......... .......... .......... ..........

421 ttgcccgcta gcatgatcgc atctgcaatc gcaatagaag tgtgcgaaaa cgctccaggg 480

421 aacgggcgat cgtactagcg tagacgttag cgttatcttc acacgctttt gcgaggtccc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttaatgatga tcccttcata atcgctgccc acgctctctt ggattttatc aatgatttcg 540

481 aattactact agggaagtat tagcgacggg tgcgagagaa cctaaaatag ttactaaagc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccctcaaaat tagtttgaaa aaactctaat tccacatcta aattgccttg tttcacgaaa 600

541 gggagtttta atcaaacttt tttgagatta aggtgtagat ttaacggaac aaagtgcttt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtttgcatga tttcatggat ttggtctaag gttaccatac cataaagcct tgggtctctg 660

601 caaacgtact aaagtaccta aaccagattc caatggtatg gtatttcgga acccagagac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(709) ..........

661 tgtcctaaca tgtttaaatt aggcccttga atcactaaaa ttttcatcat atgtatatct 720

661 acaggattgt acaaatttaa tccgggaact tagtgatttt aaaagtagta tacatataga 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc 780

721 ggaagaattt caatttgttt taataaagat ctccccttaa caataggcga gtgttaaggg 780

T7_transl_en_RBS .... .......... .......... lacO ..........

.......... .......... .......... BglII(813) .......... ..........

781 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat 840

781 gatatcactc agcataatta aagcgcccta gctctagagc taggagatgc ggcctgcgta 840

T7_pro .......... .......... .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

841 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac 900

841 gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc ggctgtagtg 900

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

901 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat 960

901 gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc cgcacccata 960

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct 1020

961 ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac gtggtaagga 1020

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc 1080

1021 acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt acgtcctcag 1080

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta 1140

1081 cgtattccct ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg aaagcgccat 1140

tet(564-300) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt 1200

1141 accgtactat cgcgggcctt ctctcagtta agtcccacca cttacacttt ggtcattgca 1200

.......... .......... .......... .......... lacI ..........

.......... .......... .......... .......... .......... ..........

1201 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 1260

1201 atatgctaca gcgtctcata cggccacaga gaatagtctg gcaaagggcg caccacttgg 1260

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 1320

1261 tccggtcggt gcaaagacgc ttttgcgccc tttttcacct tcgccgctac cgcctcgact 1320

.......... .......... lacI .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1321 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 1380

1321 taatgtaagg gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac gactaaccgc 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 1440

1381 aacggtggag gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc taatttagag 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 1500

1441 cgcggctagt tgacccacgg tcgcaccacc acagctacca tcttgcttcg ccgcagcttc 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 1560

1501 ggacatttcg ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac tagtaattga 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 1620

1561 taggcgacct actggtccta cggtaacgac accttcgacg gacgtgatta caaggccgca 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 1680

1621 ataaagaact acagagactg gtctgtgggt agttgtcata ataaaagagg gtacttctgc 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 1740

1681 catgcgctga cccgcacctc gtagaccagc gtaacccagt ggtcgtttag cgcgacaatc 1740

.......... .......... lacI ORF 3 .......... .......... ..........

ApaI(1746) .......... .......... .......... .......... ..........

1741 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 1800

1741 gcccgggtaa ttcaagacag agccgcgcag acgcagaccg accgaccgta tttatagagt 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 1860

1801 gagcgttagt ttaagtcggc tatcgccttg cccttccgct gacctcacgg tacaggccaa 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 1920

1861 aagttgtttg gtacgtttac gacttactcc cgtagcaagg gtgacgctac gaccaacggt 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 1980

1921 tgctagtcta ccgcgacccg cgttacgcgc ggtaatggct caggcccgac gcgcaaccac 1980

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 2040

1981 gcctatagag ccatcaccct atgctgctat ggcttctgtc gagtacaata tagggcggca 2040

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(2041) .......... .......... .......... .......... ..........

2041 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 2100

2041 attggtggta gtttgtccta aaagcggacg accccgtttg gtcgcacctg gcgaacgacg 2100

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 2160

2101 ttgagagagt cccggtccgc cacttcccgt tagtcgacaa cgggcagagt gaccactttt 2160

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2220

2161 ctttttggtg ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta 2220

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 2280

2221 attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc gttgcgttaa 2280

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc 2340

2281 ttacattcaa tcgagtgagt aatccgtggc cctagagctg gctacgggaa ctctcggaag 2340

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

2341 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 2400

2341 ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg tgaatactga 2400

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 2460

2401 cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca gtaaaagccg 2460

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 2520

2461 ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca taagccttag 2520

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 2580

2521 aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa gccgctcttc 2580

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg 2640

2581 gtccggtaat agcggccgta ccgccggggt gcccacgcgt actagcacga ggacagcaac 2640

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 2700

2641 tcctgggccg atccgaccgc cccaacggaa tgaccaatcg tcttacttag tggctatgcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 2760

2701 ctcgcttgca cttcgctgac gacgacgttt tgcagacgct ggactcgttg ttgtacttac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 2820

2761 cagaagccaa aggcacaaag catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 2880

2821 tacaaggcct agacgtagcg tcctacgacg accgatggga caccttgtgg atgtagacat 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 2940

2881 aattgcttcg cgaccgtaac tgggactcac taaaaagaga ccagggcggc gtaggtatgg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 3000

2941 cggtcaacaa atgggagtgt tgcaaggtca ttggcccgta caagtagtag tcattgggca 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 3060

3001 tagcactcgt aggagagagc aaagtagcca tagtaatggg ggtacttgtc tttaggggga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 3120

3061 atgtgcctcc gtagtcactg gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag 3120

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 3180

3121 tcttcggtct gtaattgcga agacctcttt gagttgctcg acctgcgcct acttgtccgt 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 3240

3181 ctgtagacac ttagcgaagt gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca 3240

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 3300

3241 aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca 3300

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 3360

3301 gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 3420

3361 acagccccgc gtcggtactg ggtcagtgca tcgctatcgc ctcacatatg accgaattga 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca 3480

3421 tacgccgtag tctcgtctaa catgactctc acgtggtata tacgccacac tttatggcgt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 3540

3481 gtctacgcat tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3600

3541 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3660

3601 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 3720

3661 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 3720

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 3780

3721 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 3840

3781 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 3900

3841 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 3960

3901 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4020

3961 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4080

4021 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 4140

4081 acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 4200

4141 tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 4260

4201 aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 4320

4261 atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4380

4321 agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4440

4381 gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4500

4441 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 4500

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 4560

4501 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 4620

4561 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 4680

4621 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 4740

4681 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... PstI(4774) .......... ..........

4741 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt 4800

4741 attatcaaac gcgttgcaac aacggtaacg acgtccgtag caccacagtg cgagcagcaa 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 4860

4801 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 4920

4861 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 4980

4921 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5040

4981 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5100

5041 cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5160

5101 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5220

5161 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5280

5221 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 5340

5281 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 5340

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5400

5341 ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt 5400

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt 5460

5401 atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggacttt aacatttgca 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 5520

5461 attataaaac aattttaagc gcaatttaaa aacaatttag tcgagtaaaa aattggttat 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 5580

5521 ccggctttag ccgttttagg gaatatttag ttttcttatc tggctctatc ccaactcaca 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 5640

5581 acaaggtcaa accttgttct caggtgataa tttcttgcac ctgaggttgc agtttcccgc 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 5700

5641 tttttggcag atagtcccgc taccgggtga tgcacttggt agtgggatta gttcaaaaaa 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 5760

5701 ccccagctcc acggcatttc gtgatttagc cttgggattt ccctcggggg ctaaatctcg 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 5820

5761 aactgcccct ttcggccgct tgcaccgctc tttccttccc ttctttcgct ttcctcgccc 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 5880

5821 gcgatcccgc gaccgttcac atcgccagtg cgacgcgcat tggtggtgtg ggcggcgcga 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 taatgcgccg ctacagggcg cgtcccattc gcca...... .......... .......... 5914

5881 attacgcggc gatgtcccgc gcagggtaag cggt...... .......... .......... 5914

.......... .......... .......... .......... .......... ..........