Sequence of pET-21a

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Sequence of pET-21a_RecR

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Sequence of pET-21a(+)-HP RecR : 5946 BP; 1452 A; 1527 C; 1530 G; 1437 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .......... XhoI(158) .......... ..........

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgctttg atccttgaat 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgaaac taggaactta 180

T7_term .......... 6xHis ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

181 taaacgccct tgagagcgaa actgagtcaa tgttttcaaa attcactcca gtaggcacgc 240

181 atttgcggga actctcgctt tgactcagtt acaaaagttt taagtgaggt catccgtgcg 240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cttgagcgat tttagtgaaa gtgaggtgga aatgctgtaa tttgtcttca atataaagca 300

241 gaactcgcta aaatcacttt cactccacct ttacgacatt aaacagaagt tatatttcgt 300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttagagaatc attagctaaa gtgggaggga aagcaaaaat gatttcttta atgttttctt 360

301 aatctcttag taatcgattt caccctccct ttcgttttta ctaaagaaat tacaaaagaa 360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caatcaggcg tttttctagg gcgttaaaat ccacttcttc tatggagttt aacacataat 420

361 gttagtccgc aaaaagatcc cgcaatttta ggtgaagaag atacctcaaa ttgtgtatta 420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcgccctaa aaaatccttt aaatcttcta aaataaacac atctcttggg tgtaaaacca 480

421 tcgcgggatt ttttaggaaa tttagaagat tttatttgtg tagagaaccc acattttggt 480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgcaaagctg agaattttgt cggctttcat cagagcaaat ctcacaaacc tcactctcgc 540

481 acgtttcgac tcttaaaaca gccgaaagta gtctcgttta gagtgtttgg agtgagagcg 540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgagcgcgtt acaagatgaa catgttttaa ggttttctag ggcgttctct aaagcgtgcg 600

541 actcgcgcaa tgttctactt gtacaaaatt ccaaaagatc ccgcaagaga tttcgcacgc 600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgatttttag cgccagatag gggttttcta aacccaaatg atacgccatt ttaaaggcgg 660

601 actaaaaatc gcggtctatc cccaaaagat ttgggtttac tatgcggtaa aatttccgcc 660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actttttacc cacattgggg attttttcta aacaatccac taaatttaaa aagtgattca 720

661 tgaaaaatgg gtgtaacccc taaaaaagat ttgttaggtg atttaaattt ttcactaagt 720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... NdeI(741) .......... .......... ..........

721 agctgttttt ataagtattc atatgtatat ctccttctta aagttaaaca aaattatttc 780

721 tcgacaaaaa tattcataag tatacatata gaggaagaat ttcaatttgt tttaataaag 780

.......... ORF 2 ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... .......... .......... ..........

781 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg 840

781 atctcccctt aacaataggc gagtgttaag gggatatcac tcagcataat taaagcgccc 840

.......... lacO .......... .......... T7_pro ..........

BglII(845) .......... .......... .......... .......... ..........

841 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 900

841 tagctctaga gctaggagat gcggcctgcg tagcaccggc cgtagtggcc gcggtgtcca 900

.......... .......... .......... ......... tet(564-300) .........

.......... .......... .......... .......... .......... ..........

901 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 960

901 cgccaacgac cgcggatata gcggctgtag tggctacccc ttctagcccg agcggtgaag 960

.......... .. tet(564-300) pBRrevBam_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

961 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 1020

961 cccgagtact cgcgaacaaa gccgcaccca taccaccgtc cggggcaccg gccccctgac 1020

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 1080

1021 aacccgcggt agaggaacgt acgtggtaag gaacgccgcc gccacgagtt gccggagttg 1080

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga 1140

1081 gatgatgacc cgacgaagga ttacgtcctc agcgtattcc ctctcgcagc tctagggcct 1140

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca 1200

1141 gtggtagctt accgcgtttt ggaaagcgcc ataccgtact atcgcgggcc ttctctcagt 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt 1260

1201 taagtcccac cacttacact ttggtcattg caatatgcta cagcgtctca tacggccaca 1260

.......... .......... .......... lacI .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg 1320

1261 gagaatagtc tggcaaaggg cgcaccactt ggtccggtcg gtgcaaagac gcttttgcgc 1320

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca 1380

1321 cctttttcac cttcgccgct accgcctcga cttaatgtaa gggttggcgc accgtgttgt 1380

.......... .......... lacI ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1381 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc 1440

1381 tgaccgcccg tttgtcagca acgactaacc gcaacggtgg aggtcagacc gggacgtgcg 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt 1500

1441 cggcagcgtt taacagcgcc gctaatttag agcgcggcta gttgacccac ggtcgcacca 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct 1560

1501 ccacagctac catcttgctt cgccgcagct tcggacattt cgccgccacg tgttagaaga 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... BclI(1581) .......... .......... ..........

1561 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc 1620

1561 gcgcgttgcg cagtcacccg actagtaatt gataggcgac ctactggtcc tacggtaacg 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc 1680

1621 acaccttcga cggacgtgat tacaaggccg caataaagaa ctacagagac tggtctgtgg 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt 1740

1681 gtagttgtca taataaaaga gggtacttct gccatgcgct gacccgcacc tcgtagacca 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... ApaI(1778) .......... ..........

1741 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg 1800

1741 gcgtaaccca gtggtcgttt agcgcgacaa tcgcccgggt aattcaagac agagccgcgc 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga 1860

1801 agacgcagac cgaccgaccg tatttataga gtgagcgtta gtttaagtcg gctatcgcct 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga 1920

1861 tgcccttccg ctgacctcac ggtacaggcc aaaagttgtt tggtacgttt acgacttact 1920

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg 1980

1921 cccgtagcaa gggtgacgct acgaccaacg gttgctagtc taccgcgacc cgcgttacgc 1980

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga 2040

1981 gcggtaatgg ctcaggcccg acgcgcaacc acgcctatag agccatcacc ctatgctgct 2040

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... HpaI(2073) .......... ..........

2041 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct 2100

2041 atggcttctg tcgagtacaa tatagggcgg caattggtgg tagtttgtcc taaaagcgga 2100

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg 2160

2101 cgaccccgtt tggtcgcacc tggcgaacga cgttgagaga gtcccggtcc gccacttccc 2160

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca 2220

2161 gttagtcgac aacgggcaga gtgaccactt ttctttttgg tgggaccgcg ggttatgcgt 2220

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 2280

2221 ttggcggaga ggggcgcgca accggctaag taattacgtc gaccgtgctg tccaaagggc 2280

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac 2340

2281 tgacctttcg cccgtcactc gcgttgcgtt aattacattc aatcgagtga gtaatccgtg 2340

lacI ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 2400

2341 gccctagagc tggctacggg aactctcgga agttgggtca gtcgaggaag gccacccgcg 2400

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 2460

2401 ccccgtactg atagcagcgg cgtgaatact gacagaagaa atagtacgtt gagcatcctg 2460

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga 2520

2461 tccacggccg tcgcgagacc cagtaaaagc cgctcctggc gaaagcgacc tcgcgctgct 2520

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 2580

2521 actagccgga cagcgaacgc cataagcctt agaacgtgcg ggagcgagtt cggaagcagt 2580

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc 2640

2581 gaccagggcg gtggtttgca aagccgctct tcgtccggta atagcggccg taccgccggg 2640

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 2700

2641 gtgcccacgc gtactagcac gaggacagca actcctgggc cgatccgacc gccccaacgg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 2760

2701 aatgaccaat cgtcttactt agtggctatg cgctcgcttg cacttcgctg acgacgacgt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 2820

2761 tttgcagacg ctggactcgt tgttgtactt accagaagcc aaaggcacaa agcatttcag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct 2880

2821 acctttgcgc cttcagtcgc gggacgtggt aatacaaggc ctagacgtag cgtcctacga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag 2940

2881 cgaccgatgg gacaccttgt ggatgtagac ataattgctt cgcgaccgta actgggactc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 3000

2941 actaaaaaga gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg 3060

3001 cattggcccg tacaagtagt agtcattggg catagcactc gtaggagaga gcaaagtagc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg 3120

3061 catagtaatg ggggtacttg tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc 3120

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

3121 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga 3180

3121 ttttttggcg ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc gaagacctct 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg 3240

3181 ttgagttgct cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc 3240

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 3300

3241 gactactcga aatggcgtcg acggagcgcg caaagccact actgccactt ttggagactg 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 3360

3301 tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc 3360

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 3420

3361 gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 3480

3421 catcgctatc gcctcacata tgaccgaatt gatacgccgt agtctcgtct aacatgactc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 3540

3481 tcacgtggta tatacgccac actttatggc gtgtctacgc attcctcttt tatggcgtag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3600

3541 tccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3660

3601 cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3720

3661 cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3780

3721 gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3840

3781 gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3900

3841 gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 3960

3901 agcccttcgc accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4020

3961 caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4080

4021 aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4140

4081 cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 4200

4141 accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4260

4201 ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4320

4261 tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4380

4321 ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc 4380

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4440

4381 taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt aatttttact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4500

4441 tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat 4500

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

4501 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4560

4501 tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4620

4561 gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4680

4621 tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4740

4681 tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4800

4741 acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

PstI(4806) .......... .......... .......... .......... ..........

4801 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4860

4801 cgacgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4920

4861 gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4980

4921 ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 5040

4981 cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 5100

5041 atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5160

5101 agttatgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5220

5161 gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5280

5221 gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5340

5281 cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact 5340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5400

5341 tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac 5400

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5401 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5460

5401 tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa 5460

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 5520

5461 ggggcttttc acggtggact ttaacatttg caattataaa acaattttaa gcgcaattta 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 5580

5521 aaaacaattt agtcgagtaa aaaattggtt atccggcttt agccgtttta gggaatattt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 5640

5581 agttttctta tctggctcta tcccaactca caacaaggtc aaaccttgtt ctcaggtgat 5640

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

5641 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 5700

5641 aatttcttgc acctgaggtt gcagtttccc gctttttggc agatagtccc gctaccgggt 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 5760

5701 gatgcacttg gtagtgggat tagttcaaaa aaccccagct ccacggcatt tcgtgattta 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 5820

5761 gccttgggat ttccctcggg ggctaaatct cgaactgccc ctttcggccg cttgcaccgc 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 5880

5821 tctttccttc ccttctttcg ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 5940

5881 tgcgacgcgc attggtggtg tgggcggcgc gaattacgcg gcgatgtccc gcgcagggta 5940

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tcgcca.... .......... .......... .......... .......... .......... 5946

5941 agcggt.... .......... .......... .......... .......... .......... 5946

.......... .......... .......... .......... .......... ..........