Sequence of pET-22b(+)

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Sequence of pET-22b(+)

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Sequence of pET-22b(+) : 5493 BP; 1302 A; 1456 C; 1461 G; 1274 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 240

181 gctgcctcga gcttaagcct aggcttaatt aaggctatag gtaccggtag cggccgaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(288) ..........

241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 300

241 gtcgctcctc gtcgtctggt cgtcgtcgcc agccgtcgtc cataaagtat acatatagag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 360

301 gaagaatttc aatttgtttt aataaagatc tccccttaac aataggcgag tgttaagggg 360

T7_transl_en_RBS .... .......... .......... lacO ..........

.......... .......... .......... BglII(392) .......... ..........

361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 420

361 atatcactca gcataattaa agcgccctag ctctagagct aggagatgcg gcctgcgtag 420

T7_pro .......... .......... .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 480

421 caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg gctgtagtgg 480

.......... ......... tet(564-300) ....... pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 540

481 ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc gcacccatac 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 600

541 caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaacgtacg tggtaaggaa 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 660

601 cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta cgtcctcagc 660

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 720

661 gtattccctc tcgcagctct agggcctgtg gtagcttacc gcgttttgga aagcgccata 720

tet(564-300) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 780

721 ccgtactatc gcgggccttc tctcagttaa gtcccaccac ttacactttg gtcattgcaa 780

.......... .......... .......... .......... lacI ..........

.......... .......... .......... .......... .......... ..........

781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 840

781 tatgctacag cgtctcatac ggccacagag aatagtctgg caaagggcgc accacttggt 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 900

841 ccggtcggtg caaagacgct tttgcgccct ttttcacctt cgccgctacc gcctcgactt 900

.......... .......... lacI .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 960

901 aatgtaaggg ttggcgcacc gtgttgttga ccgcccgttt gtcagcaacg actaaccgca 960

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 1020

961 acggtggagg tcagaccggg acgtgcgcgg cagcgtttaa cagcgccgct aatttagagc 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 1080

1021 gcggctagtt gacccacggt cgcaccacca cagctaccat cttgcttcgc cgcagcttcg 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... BclI(1128) ..........

1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 1140

1081 gacatttcgc cgccacgtgt tagaagagcg cgttgcgcag tcacccgact agtaattgat 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 1200

1141 aggcgaccta ctggtcctac ggtaacgaca ccttcgacgg acgtgattac aaggccgcaa 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 1260

1201 taaagaacta cagagactgg tctgtgggta gttgtcataa taaaagaggg tacttctgcc 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 1320

1261 atgcgctgac ccgcacctcg tagaccagcg taacccagtg gtcgtttagc gcgacaatcg 1320

.......... .......... lacI ORF 2 .......... .......... ..........

ApaI(1325) .......... .......... .......... .......... ..........

1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 1380

1321 cccgggtaat tcaagacaga gccgcgcaga cgcagaccga ccgaccgtat ttatagagtg 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 1440

1381 agcgttagtt taagtcggct atcgccttgc ccttccgctg acctcacggt acaggccaaa 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 1500

1441 agttgtttgg tacgtttacg acttactccc gtagcaaggg tgacgctacg accaacggtt 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 1560

1501 gctagtctac cgcgacccgc gttacgcgcg gtaatggctc aggcccgacg cgcaaccacg 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(1620)

1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 1620

1561 cctatagagc catcacccta tgctgctatg gcttctgtcg agtacaatat agggcggcaa 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 1680

1621 ttggtggtag tttgtcctaa aagcggacga ccccgtttgg tcgcacctgg cgaacgacgt 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 1740

1681 tgagagagtc ccggtccgcc acttcccgtt agtcgacaac gggcagagtg accacttttc 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1800

1741 tttttggtgg gaccgcgggt tatgcgtttg gcggagaggg gcgcgcaacc ggctaagtaa 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1860

1801 ttacgtcgac cgtgctgtcc aaagggctga cctttcgccc gtcactcgcg ttgcgttaat 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 1920

1861 tacattcaat cgagtgagta atccgtggcc ctagagctgg ctacgggaac tctcggaagt 1920

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 1980

1921 tgggtcagtc gaggaaggcc acccgcgccc cgtactgata gcagcggcgt gaatactgac 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 2040

1981 agaagaaata gtacgttgag catcctgtcc acggccgtcg cgagacccag taaaagccgc 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 2100

2041 tcctggcgaa agcgacctcg cgctgctact agccggacag cgaacgccat aagccttaga 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 2160

2101 acgtgcggga gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag ccgctcttcg 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 2220

2161 tccggtaata gcggccgtac cgccggggtg cccacgcgta ctagcacgag gacagcaact 2220

tet(852-576) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 2280

2221 cctgggccga tccgaccgcc ccaacggaat gaccaatcgt cttacttagt ggctatgcgc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 2340

2281 tcgcttgcac ttcgctgacg acgacgtttt gcagacgctg gactcgttgt tgtacttacc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 2400

2341 agaagccaaa ggcacaaagc atttcagacc tttgcgcctt cagtcgcggg acgtggtaat 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 2460

2401 acaaggccta gacgtagcgt cctacgacga ccgatgggac accttgtgga tgtagacata 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 2520

2461 attgcttcgc gaccgtaact gggactcact aaaaagagac cagggcggcg taggtatggc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 2580

2521 ggtcaacaaa tgggagtgtt gcaaggtcat tggcccgtac aagtagtagt cattgggcat 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 2640

2581 agcactcgta ggagagagca aagtagccat agtaatgggg gtacttgtct ttagggggaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 2700

2641 tgtgcctccg tagtcactgg tttgtccttt tttggcggga attgtaccgg gcgaaatagt 2700

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 2760

2701 cttcggtctg taattgcgaa gacctctttg agttgctcga cctgcgccta cttgtccgtc 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 2820

2761 tgtagacact tagcgaagtg ctggtgcgac tactcgaaat ggcgtcgacg gagcgcgcaa 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2880

2821 agccactact gccacttttg gagactgtgt acgtcgaggg cctctgccag tgtcgaacag 2880

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2940

2881 acattcgcct acggccctcg tctgttcggg cagtcccgcg cagtcgccca caaccgccca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 3000

2941 cagccccgcg tcggtactgg gtcagtgcat cgctatcgcc tcacatatga ccgaattgat 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 3060

3001 acgccgtagt ctcgtctaac atgactctca cgtggtatat acgccacact ttatggcgtg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 3120

3061 tctacgcatt cctcttttat ggcgtagtcc gcgagaaggc gaaggagcga gtgactgagc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3180

3121 gacgcgagcc agcaagccga cgccgctcgc catagtcgag tgagtttccg ccattatgcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3240

3181 aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3300

3241 cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg 3300

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3360

3301 ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc tgatatttct 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3420

3361 atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg ggacggcgaa 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3480

3421 tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3540

3481 acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca cgtgcttggg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3600

3541 gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag gttgggccat 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3660

3601 tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 3720

3661 catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg atcttcctgt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3780

3721 cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca accatcgaga 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3840

3781 actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3900

3841 tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc cagactgcga 3900

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 3960

3901 gtcaccttgc ttttgagtgc aattccctaa aaccagtact ctaatagttt ttcctagaag 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4020

3961 tggatctagg aaaatttaat ttttacttca aaatttagtt agatttcata tatactcatt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 4080

4021 tgaaccagac tgtcaatggt tacgaattag tcactccgtg gatagagtcg ctagacagat 4080

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 4140

4081 aaagcaagta ggtatcaacg gactgagggg cagcacatct attgatgcta tgccctcccg 4140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 4200

4141 aatggtagac cggggtcacg acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 4260

4201 aatagtcgtt atttggtcgg tcggccttcc cggctcgcgt cttcaccagg acgttgaaat 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 4320

4261 aggcggaggt aggtcagata attaacaacg gcccttcgat ctcattcatc aagcggtcaa 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... PstI(4353) .......... ..........

4321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 4380

4321 ttatcaaacg cgttgcaaca acggtaacga cgtccgtagc accacagtgc gagcagcaaa 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 4440

4381 ccataccgaa gtaagtcgag gccaagggtt gctagttccg ctcaatgtac tagggggtac 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 4500

4441 aacacgtttt ttcgccaatc gaggaagcca ggaggctagc aacagtcttc attcaaccgg 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 4560

4501 cgtcacaata gtgagtacca ataccgtcgt gacgtattaa gagaatgaca gtacggtagg 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 4620

4561 cattctacga aaagacactg accactcatg agttggttca gtaagactct tatcacatac 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4680

4621 gccgctggct caacgagaac gggccgcagt tatgccctat tatggcgcgg tgtatcgtct 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 4740

4681 tgaaattttc acgagtagta accttttgca agaagccccg cttttgagag ttcctagaat 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 4800

4741 ggcgacaact ctaggtcaag ctacattggg tgagcacgtg ggttgactag aagtcgtaga 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 4860

4801 aaatgaaagt ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg gcgttttttc 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 4920

4861 ccttattccc gctgtgcctt tacaacttat gagtatgaga aggaaaaagt tataataact 4920

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 4980

4921 tcgtaaatag tcccaataac agagtactcg cctatgtata aacttacata aatcttttta 4980

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt 5040

4981 tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg gtggacttta acatttgcaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 5100

5041 ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa attggttatc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 5160

5101 cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc caactcacaa 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 5220

5161 caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca gtttcccgct 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 5280

5221 ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag ttcaaaaaac 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 5340

5281 cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc taaatctcga 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 5400

5341 actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt tcctcgcccg 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 5460

5401 cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aatgcgccgc tacagggcgc gtcccattcg cca....... .......... .......... 5493

5461 ttacgcggcg atgtcccgcg cagggtaagc ggt....... .......... .......... 5493

.......... .......... .......... .......... .......... ..........