Sequence of pET-22bPlus

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Sequence of pET-22bPlus

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Sequence of pET-22b(+)-HpENR : 6238 BP; 1521 A; 1652 C; 1567 G; 1498 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166) HindIII(173) SalI(179)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccttattgt tctttatgca aatcccacag tagcgtagct 240

181 gctgcctcga gcttaagcct aggaataaca agaaatacgt ttagggtgtc atcgcatcga 240

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

241 ttattatctt tttcttccac agcccccatg cccataacat gatagccagc atccacaaaa 300

241 aataatagaa aaagaaggtg tcgggggtac gggtattgta ctatcggtcg taggtgtttt 300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tgcacttccc cactcacccc gctagacaaa ctagagagca aatacatccc ggcattgccc 360

301 acgtgaaggg gtgagtgggg cgatctgttt gatctctcgt ttatgtaggg ccgtaacggg 360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acttcttcta aactcacatt ttttcttaaa ggggcgttga tttcattcca ttttaaaatc 420

361 tgaagaagat ttgagtgtaa aaaagaattt ccccgcaact aaagtaaggt aaaattttag 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attctaaaat cagcgatccc gctagaagcg agcgtcctaa taggcccggc cgatagggca 480

421 taagatttta gtcgctaggg cgatcttcgc tcgcaggatt atccgggccg gctatcccgt 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttcactctta tatggtgttt gcctaaatcc accgctaaat aacgcaccgc actctctagg 540

481 aagtgagaat ataccacaaa cggatttagg tggcgattta ttgcgtggcg tgagagatcc 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gccgctttag ccaaccccat cacattgtaa tgcgccatgt atttggtgct acccaaatag 600

541 cggcgaaatc ggttggggta gtgtaacatt acgcggtaca taaaccacga tgggtttatc 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cttagagtca aaacagacgc tccgttattc aataaaggtt ttagggtgtt tgtcagctct 660

601 gaatctcagt tttgtctgcg aggcaataag ttatttccaa aatcccacaa acagtcgaga 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 attaaagaat aaacagaaat ttccatagcg gtgttaaacg cgcttttaga agtttccaac 720

661 taatttctta tttgtcttta aaggtatcgc cacaatttgc gcgaaaatct tcaaaggttg 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aagctcccct ctaaagcctc tttaggggca aaggccacgc tatgaacaat aaaatccaat 780

721 ttcgagggga gatttcggag aaatccccgt ttccggtgcg atacttgtta ttttaggtta 780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagcctaaat cctttttaac gctattgtat agcgacttga aatgctcttc tttgctcaca 840

781 ctcggattta ggaaaaattg cgataacata tcgctgaact ttacgagaag aaacgagtgt 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... StuI(884) ..........

841 tccaattcat acacataggg gctattcaat tcctgcgcga taggccttac gcgcttttct 900

841 aggttaagta tgtgtatccc cgataagtta aggacgcgct atccggaatg cgcgaaaaga 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aaactctcat tcaaataagt gaaagccaaa gtagcccctt gattgaaaca agattgagcg 960

901 tttgagagta agtttattca ctttcggttt catcggggaa ctaactttgt tctaactcgc 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atcccataag cgatggattt attgttcgcc acccctacaa taagcccttt tttacctttt 1020

961 tagggtattc gctacctaaa taacaagcgg tggggatgtt attcgggaaa aaatggaaaa 1020

.......... .......... ORF 3 .......... .......... ..........

.......... NdeI(1033) .......... .......... .......... ..........

1021 aaaaatccca tcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg 1080

1021 tttttagggt agtatacata tagaggaaga atttcaattt gttttaataa agatctcccc 1080

ORF 3 .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... .......... ......... BglII(1137)

1081 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat 1140

1081 ttaacaatag gcgagtgtta aggggatatc actcagcata attaaagcgc cctagctcta 1140

.......... lacO .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc 1200

1141 gagctaggag atgcggcctg cgtagcaccg gccgtagtgg ccgcggtgtc cacgccaacg 1200

.......... .......... ......... tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

1201 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat 1260

1201 accgcggata tagcggctgt agtggctacc ccttctagcc cgagcggtga agcccgagta 1260

....... pBRrevBam_pri tet(564-300) ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 1320

1261 ctcgcgaaca aagccgcacc cataccaccg tccggggcac cggccccctg acaacccgcg 1320

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact 1380

1321 gtagaggaac gtacgtggta aggaacgccg ccgccacgag ttgccggagt tggatgatga 1380

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg 1440

1381 cccgacgaag gattacgtcc tcagcgtatt ccctctcgca gctctagggc ctgtggtagc 1440

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg 1500

1441 ttaccgcgtt ttggaaagcg ccataccgta ctatcgcggg ccttctctca gttaagtccc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc 1560

1501 accacttaca ctttggtcat tgcaatatgc tacagcgtct catacggcca cagagaatag 1560

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag 1620

1561 tctggcaaag ggcgcaccac ttggtccggt cggtgcaaag acgcttttgc gccctttttc 1620

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg 1680

1621 accttcgccg ctaccgcctc gacttaatgt aagggttggc gcaccgtgtt gttgaccgcc 1680

.......... .......... lacI ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc 1740

1681 cgtttgtcag caacgactaa ccgcaacggt ggaggtcaga ccgggacgtg cgcggcagcg 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga 1800

1741 tttaacagcg ccgctaattt agagcgcggc tagttgaccc acggtcgcac caccacagct 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac 1860

1801 accatcttgc ttcgccgcag cttcggacat ttcgccgcca cgtgttagaa gagcgcgttg 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... BclI(1873) .......... .......... .......... ..........

1861 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag 1920

1861 cgcagtcacc cgactagtaa ttgataggcg acctactggt cctacggtaa cgacaccttc 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca 1980

1921 gacggacgtg attacaaggc cgcaataaag aactacagag actggtctgt gggtagttgt 1980

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg 2040

1981 cataataaaa gagggtactt ctgccatgcg ctgacccgca cctcgtagac cagcgtaacc 2040

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... ApaI(2070) .......... .......... ..........

2041 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc 2100

2041 cagtggtcgt ttagcgcgac aatcgcccgg gtaattcaag acagagccgc gcagacgcag 2100

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag 2160

2101 accgaccgac cgtatttata gagtgagcgt tagtttaagt cggctatcgc cttgcccttc 2160

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg 2220

2161 cgctgacctc acggtacagg ccaaaagttg tttggtacgt ttacgactta ctcccgtagc 2220

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta 2280

2221 aagggtgacg ctacgaccaa cggttgctag tctaccgcga cccgcgttac gcgcggtaat 2280

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag 2340

2281 ggctcaggcc cgacgcgcaa ccacgcctat agagccatca ccctatgctg ctatggcttc 2340

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... HpaI(2365) .......... .......... ..........

2341 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc 2400

2341 tgtcgagtac aatatagggc ggcaattggt ggtagtttgt cctaaaagcg gacgaccccg 2400

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc 2460

2401 tttggtcgca cctggcgaac gacgttgaga gagtcccggt ccgccacttc ccgttagtcg 2460

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct 2520

2461 acaacgggca gagtgaccac ttttcttttt ggtgggaccg cgggttatgc gtttggcgga 2520

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 2580

2521 gaggggcgcg caaccggcta agtaattacg tcgaccgtgc tgtccaaagg gctgaccttt 2580

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct 2640

2581 cgcccgtcac tcgcgttgcg ttaattacat tcaatcgagt gagtaatccg tggccctaga 2640

lacI ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 2700

2641 gctggctacg ggaactctcg gaagttgggt cagtcgagga aggccacccg cgccccgtac 2700

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

2701 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 2760

2701 tgatagcagc ggcgtgaata ctgacagaag aaatagtacg ttgagcatcc tgtccacggc 2760

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 2820

2761 cgtcgcgaga cccagtaaaa gccgctcctg gcgaaagcga cctcgcgctg ctactagccg 2820

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 2880

2821 gacagcgaac gccataagcc ttagaacgtg cgggagcgag ttcggaagca gtgaccaggg 2880

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg 2940

2881 cggtggtttg caaagccgct cttcgtccgg taatagcggc cgtaccgccg gggtgcccac 2940

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt 3000

2941 gcgtactagc acgaggacag caactcctgg gccgatccga ccgccccaac ggaatgacca 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct 3060

3001 atcgtcttac ttagtggcta tgcgctcgct tgcacttcgc tgacgacgac gttttgcaga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg 3120

3061 cgctggactc gttgttgtac ttaccagaag ccaaaggcac aaagcatttc agacctttgc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta 3180

3121 gccttcagtc gcgggacgtg gtaatacaag gcctagacgt agcgtcctac gacgaccgat 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt 3240

3181 gggacacctt gtggatgtag acataattgc ttcgcgaccg taactgggac tcactaaaaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg 3300

3241 gagaccaggg cggcgtaggt atggcggtca acaaatggga gtgttgcaag gtcattggcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt 3360

3301 cgtacaagta gtagtcattg ggcatagcac tcgtaggaga gagcaaagta gccatagtaa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc 3420

3361 tgggggtact tgtctttagg gggaatgtgc ctccgtagtc actggtttgt ccttttttgg 3420

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

3421 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac 3480

3421 cgggaattgt accgggcgaa atagtcttcg gtctgtaatt gcgaagacct ctttgagttg 3480

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag 3540

3481 ctcgacctgc gcctacttgt ccgtctgtag acacttagcg aagtgctggt gcgactactc 3540

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 3600

3541 gaaatggcgt cgacggagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc 3600

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3601 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 3660

3601 gagggcctct gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat 3720

3661 ccgcgcagtc gcccacaacc gcccacagcc ccgcgtcggt actgggtcag tgcatcgcta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc 3780

3721 tcgcctcaca tatgaccgaa ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 atatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 3840

3781 tatatacgcc acactttatg gcgtgtctac gcattcctct tttatggcgt agtccgcgag 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3900

3841 aaggcgaagg agcgagtgac tgagcgacgc gagccagcaa gccgacgccg ctcgccatag 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3960

3901 tcgagtgagt ttccgccatt atgccaatag gtgtcttagt cccctattgc gtcctttctt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4020

3961 gtacactcgt tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa 4020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4080

4021 aaaggtatcc gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4140

4081 cgctttgggc tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4200

4141 gagaggacaa ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4260

4201 gcaccgcgaa agagtatcga gtgcgacatc catagagtca agccacatcc agcaagcgag 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4320

4261 gttcgacccg acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4380

4321 gatagcagaa ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4440

4381 attgtcctaa tcgtctcgct ccatacatcc gccacgatgt ctcaagaact tcaccaccgg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 4500

4441 attgatgccg atgtgatctt cctgtcataa accatagacg cgagacgact tcggtcaatg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4560

4501 gaagcctttt tctcaaccat cgagaactag gccgtttgtt tggtggcgac catcgccacc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4620

4561 aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4680

4621 ctagaaaaga tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4740

4681 gtactctaat agtttttcct agaagtggat ctaggaaaat ttaattttta cttcaaaatt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4800

4741 tagttagatt tcatatatac tcatttgaac cagactgtca atggttacga attagtcact 4800

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4860

4801 ccgtggatag agtcgctaga cagataaagc aagtaggtat caacggactg aggggcagca 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4920

4861 catctattga tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt actatggcgc 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4980

4921 tctgggtgcg agtggccgag gtctaaatag tcgttatttg gtcggtcggc cttcccggct 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5040

4981 cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc agataattaa caacggccct 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... PstI(5098)

5041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg 5100

5041 tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg caacaacggt aacgacgtcc 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5160

5101 gtagcaccac agtgcgagca gcaaaccata ccgaagtaag tcgaggccaa gggttgctag 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5220

5161 ttccgctcaa tgtactaggg ggtacaacac gttttttcgc caatcgagga agccaggagg 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5280

5221 ctagcaacag tcttcattca accggcgtca caatagtgag taccaatacc gtcgtgacgt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 5340

5281 attaagagaa tgacagtacg gtaggcattc tacgaaaaga cactgaccac tcatgagttg 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 5400

5341 gttcagtaag actcttatca catacgccgc tggctcaacg agaacgggcc gcagttatgc 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 5460

5401 cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 5520

5461 ccccgctttt gagagttcct agaatggcga caactctagg tcaagctaca ttgggtgagc 5520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 5580

5521 acgtgggttg actagaagtc gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg 5580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 5640

5581 tccttccgtt ttacggcgtt ttttccctta ttcccgctgt gcctttacaa cttatgagta 5640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 5700

5641 tgagaaggaa aaagttataa taacttcgta aatagtccca ataacagagt actcgcctat 5700

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 5760

5701 gtataaactt acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt 5760

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agtgccacct gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta 5820

5761 tcacggtgga ctttaacatt tgcaattata aaacaatttt aagcgcaatt taaaaacaat 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 5880

5821 ttagtcgagt aaaaaattgg ttatccggct ttagccgttt tagggaatat ttagttttct 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 5940

5881 tatctggctc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt 5940

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

5941 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 6000

5941 gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg gtgatgcact 6000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 6060

6001 tggtagtggg attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg 6060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 6120

6061 atttccctcg ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc gctctttcct 6120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 6180

6121 tcccttcttt cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc 6180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca.. 6238

6181 gcattggtgg tgtgggcggc gcgaattacg cggcgatgtc ccgcgcaggg taagcggt.. 6238

.......... f1_origin .......... .......... .......... ..........