Sequence of pET-23(+)

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Sequence of pET-23(+)

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Sequence of pET-23(+) : 3592 BP; 898 A; 917 C; 917 G; 860 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

..... SacI(190),EcoRI(192) ..... .......... .......... ..........

181 cgacggagct cgaattcgga tcctagaggg aaaccgttgt ggtctcccta tagtgagtcg 240

181 gctgcctcga gcttaagcct aggatctccc tttggcaaca ccagagggat atcactcagc 240

.......... .......... .......... .......... .......... T7_pro

.......... BglII(260) .......... .......... MscI(285) ..........

241 tattaatttc gcgggatcga gatctcgggc agcgttgggt cctggccacg ggtgcgcatg 300

241 ataattaaag cgccctagct ctagagcccg tcgcaaccca ggaccggtgc ccacgcgtac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 360

301 tagcacgagg acagcaactc ctgggccgat ccgaccgccc caacggaatg accaatcgtc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 420

361 ttacttagtg gctatgcgct cgcttgcact tcgctgacga cgacgttttg cagacgctgg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 480

421 actcgttgtt gtacttacca gaagccaaag gcacaaagca tttcagacct ttgcgccttc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt 540

481 agtcgcggga cgtggtaata caaggcctag acgtagcgtc ctacgacgac cgatgggaca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg 600

541 ccttgtggat gtagacataa ttgcttcgcg accgtaactg ggactcacta aaaagagacc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt 660

601 agggcggcgt aggtatggcg gtcaacaaat gggagtgttg caaggtcatt ggcccgtaca 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc 720

661 agtagtagtc attgggcata gcactcgtag gagagagcaa agtagccata gtaatggggg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt 780

721 tacttgtctt tagggggaat gtgcctccgt agtcactggt ttgtcctttt ttggcgggaa 780

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

781 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg 840

781 ttgtaccggg cgaaatagtc ttcggtctgt aattgcgaag acctctttga gttgctcgac 840

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac 900

841 ctgcgcctac ttgtccgtct gtagacactt agcgaagtgc tggtgcgact actcgaaatg 900

.......... .......... ROP .......... .......... ..........

PvuII(905) .......... .......... .......... .......... ..........

901 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 960

901 gcgtcgacgg agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc 960

.......... .......... ROP .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

961 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 1020

961 ctctgccagt gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 1080

1021 agtcgcccac aaccgcccac agccccgcgt cggtactggg tcagtgcatc gctatcgcct 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 1140

1081 cacatatgac cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtggtatata 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 1200

1141 cgccacactt tatggcgtgt ctacgcattc ctcttttatg gcgtagtccg cgagaaggcg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1260

1201 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1320

1261 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1380

1321 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 1380

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 1440

1381 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 1500

1441 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 1560

1501 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 1620

1561 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 1680

1621 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 1740

1681 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1800

1741 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1860

1801 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1920

1861 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1980

1921 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 2040

1981 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 2100

2041 taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 2160

2101 gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg 2160

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 2220

2161 atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2280

2221 ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 2340

2281 tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 2400

2341 ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... PstI(2452)

2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 2460

2401 tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgac gtccgtagca 2460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2520

2461 ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc 2520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2580

2521 tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca 2580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2640

2581 acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2700

2641 agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2760

2701 taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2820

2761 atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2880

2821 ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2940

2881 gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 3000

2941 cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3060

3001 ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa 3060

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 3120

3061 acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3180

3121 tggactttaa catttgcaat tataaaacaa ttttaagcgc aatttaaaaa caatttagtc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3240

3181 gagtaaaaaa ttggttatcc ggctttagcc gttttaggga atatttagtt ttcttatctg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3300

3241 gctctatccc aactcacaac aaggtcaaac cttgttctca ggtgataatt tcttgcacct 3300

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3360

3301 gaggttgcag tttcccgctt tttggcagat agtcccgcta ccgggtgatg cacttggtag 3360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3420

3361 tgggattagt tcaaaaaacc ccagctccac ggcatttcgt gatttagcct tgggatttcc 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3480

3421 ctcgggggct aaatctcgaa ctgccccttt cggccgcttg caccgctctt tccttccctt 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3540

3481 ctttcgcttt cctcgcccgc gatcccgcga ccgttcacat cgccagtgcg acgcgcattg 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca........ 3592

3541 gtggtgtggg cggcgcgaat tacgcggcga tgtcccgcgc agggtaagcg gt........ 3592

.......... f1_origin .......... .......... .......... ..........