\LOCUS pET-23 a,b,c,d(+) 3666 bp DNA SYN 03-Sep-2007 DEFINITION pET-23 a,b,c,d(+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3666 /organism="pET-23 a,b,c,d(+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(207..239) /label="T7_gene10_leader" misc_feature complement(207..239) /label="T7_leader" misc_feature complement(222..240) /label="Xpress_fwd_primer" misc_feature complement(248..264) /label="T7_transl_en_RBS" promoter complement(301..319) /label="T7_promoter" misc_feature 828..1019 /label="ROP" misc_feature complement(1013..1035) /label="pGEX_3_primer" rep_origin complement(1434..2053) /label="pBR322_origin" CDS complement(2208..3068) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2208..3068) /label="Ampicillin" /gene="Ampicillin" promoter complement(3110..3138) /label="AmpR_promoter" rep_origin complement(3332..3638) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata 241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc 301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc 361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct 601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag 661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg 781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg 841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga 901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg 961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 3661 tcgcca //tpET-23 a,b,c,d(+) "Times New RomanCtT7_term"Times New RomanCtEWT7_Terminal_pri"Times New RomanCɆt6xHis"Times New RomantT7_gene10_leader"Times New RomanCt T7_leader"Times New RomanCtXpress_fwd_pri"Times New Roman*CɆtT7_transl_en_RBS"Times New Romant-?T7_pro"Times New RomanCt<ROP"Times New RomanCt  pGEX_3_pri"Times New RomanCɆt pBR322_origin"Times New Romant ORF 3"Times New RomanCt  Ampicillin"Times New Roman(Ct& B AmpR_pro"Times New RomanCɆt 6 f1_origin"Times New Roman rXhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),NheI(231),NdeI(238),XbaI(276)"Times New Roman tQ/ BglII(334),MscI(359)"Times New RomanNt  _ PvuII"Times New Romant PstI"Times New Roman t } test"Times New Romant-t