Sequence of pET-23_a_b_c

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Sequence of pET-23_a_b_c_d(+)

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Sequence of pET-23 a,b,c,d(+) : 3666 BP; 919 A; 936 C; 926 G; 885 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... NheI(231)

181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata 240

181 gctgcctcga gcttaagcct aggcgctggg taaacgacag gtggtcagta cgatcggtat 240

.......... .......... .......... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... XbaI(276) .......... ..........

241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc 300

241 acatatagag gaagaatttc aatttgtttt aataaagatc tccctttggc aacaccagag 300

....... T7_transl_en_RBS ....... .......... .......... ..........

.......... .......... .......... BglII(334) .......... MscI(359)

301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc 360

301 ggatatcact cagcataatt aaagcgccct agctctagag cccgtcgcaa cccaggaccg 360

T7_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 420

361 gtgcccacgc gtactagcac gaggacagca actcctgggc cgatccgacc gccccaacgg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 480

421 aatgaccaat cgtcttactt agtggctatg cgctcgcttg cacttcgctg acgacgacgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 540

481 tttgcagacg ctggactcgt tgttgtactt accagaagcc aaaggcacaa agcatttcag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct 600

541 acctttgcgc cttcagtcgc gggacgtggt aatacaaggc ctagacgtag cgtcctacga 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag 660

601 cgaccgatgg gacaccttgt ggatgtagac ataattgctt cgcgaccgta actgggactc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 720

661 actaaaaaga gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg 780

721 cattggcccg tacaagtagt agtcattggg catagcactc gtaggagaga gcaaagtagc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg 840

781 catagtaatg ggggtacttg tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc 840

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga 900

841 ttttttggcg ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc gaagacctct 900

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg 960

901 ttgagttgct cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc 960

.......... .......... ROP .......... .......... ..........

.......... PvuII(979) .......... .......... .......... ..........

961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 1020

961 gactactcga aatggcgtcg acggagcgcg caaagccact actgccactt ttggagactg 1020

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 1080

1021 tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc 1080

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 1140

1081 gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 1200

1141 catcgctatc gcctcacata tgaccgaatt gatacgccgt agtctcgtct aacatgactc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 1260

1201 tcacgtggta tatacgccac actttatggc gtgtctacgc attcctcttt tatggcgtag 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 1320

1261 tccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 1380

1321 cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1440

1381 cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1500

1441 gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1560

1501 gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1620

1561 gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1680

1621 agcccttcgc accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1740

1681 caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 1800

1741 aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 1860

1801 cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 1920

1861 accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1980

1921 ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 2040

1981 tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2100

2041 ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc 2100

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2160

2101 taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt aatttttact 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2220

2161 tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat 2220

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2280

2221 tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag 2280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2340

2281 gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac 2340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2400

2341 tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct 2400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2460

2401 tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca 2460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2520

2461 acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa 2520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

PstI(2526) .......... .......... .......... .......... ..........

2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2580

2521 cgacgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2640

2581 gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2700

2641 ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 2760

2701 cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 2820

2761 atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 2880

2821 agttatgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 2940

2881 gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3000

2941 gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3060

3001 cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3120

3061 tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac 3120

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3180

3121 tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa 3180

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat 3240

3181 ggggcttttc acggtggact ttaacatttg caattataaa acaattttaa gcgcaattta 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 3300

3241 aaaacaattt agtcgagtaa aaaattggtt atccggcttt agccgtttta gggaatattt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 3360

3301 agttttctta tctggctcta tcccaactca caacaaggtc aaaccttgtt ctcaggtgat 3360

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 3420

3361 aatttcttgc acctgaggtt gcagtttccc gctttttggc agatagtccc gctaccgggt 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 3480

3421 gatgcacttg gtagtgggat tagttcaaaa aaccccagct ccacggcatt tcgtgattta 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 3540

3481 gccttgggat ttccctcggg ggctaaatct cgaactgccc ctttcggccg cttgcaccgc 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 3600

3541 tctttccttc ccttctttcg ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat 3660

3601 tgcgacgcgc attggtggtg tgggcggcgc gaattacgcg gcgatgtccc gcgcagggta 3660

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgcca.... .......... .......... .......... .......... .......... 3666

3661 agcggt.... .......... .......... .......... .......... .......... 3666

.......... .......... .......... .......... .......... ..........