Sequence of pET-24_b

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Sequence of pET-24_b_(+)

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Sequence of pET-24 b (+): 5309 BP; 1260 A; 1374 C; 1421 G; 1254 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ NheI(230) ..........

181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 240

181 gctgcctcga gcttaagcct agggctgggt aaacgacagg tggtcagtac gatcggtata 240

.......... .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... XbaI(275) .......... ..........

241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 300

241 catatagagg aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt 300

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... .......... BglII(341) ..........

301 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 360

301 gttaagggga tatcactcag cataattaaa gcgccctagc tctagagcta ggagatgcgg 360

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 420

361 cctgcgtagc accggccgta gtggccgcgg tgtccacgcc aacgaccgcg gatatagcgg 420

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 480

421 ctgtagtggc taccccttct agcccgagcg gtgaagcccg agtactcgcg aacaaagccg 480

pBRrevBam_pri ..... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 540

481 cacccatacc accgtccggg gcaccggccc cctgacaacc cgcggtagag gaacgtacgt 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 600

541 ggtaaggaac gccgccgcca cgagttgccg gagttggatg atgacccgac gaaggattac 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 660

601 gtcctcagcg tattccctct cgcagctcta gggcctgtgg tagcttaccg cgttttggaa 660

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 720

661 agcgccatac cgtactatcg cgggccttct ctcagttaag tcccaccact tacactttgg 720

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

721 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 780

721 tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc aaagggcgca 780

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 840

781 ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc gccgctaccg 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 900

841 cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg tcagcaacga 900

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 960

901 ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac agcgccgcta 960

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1020

961 atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc ttgcttcgcc 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1077)

1021 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1080

1021 gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt cacccgacta 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1140

1081 gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga cgtgattaca 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1200

1141 aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat aaaagagggt 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1260

1201 acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg tcgtttagcg 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1274) .......... .......... .......... ..........

1261 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1320

1261 cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac cgaccgtatt 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1380

1321 tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga cctcacggta 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1440

1381 caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt gacgctacga 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1500

1441 ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca ggcccgacgc 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1560

1501 gcaaccacgc ctatagagcc atcaccctat gctgctatgg cttctgtcga gtacaatata 1560

.......... .......... lacI ORF 2 .......... .......... ..........

HpaI(1569) .......... .......... .......... .......... ..........

1561 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1620

1561 gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt cgcacctggc 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1680

1621 gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg ggcagagtga 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1740

1681 ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1800

1741 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1860

1801 tgcgttaatt acattcaatc gagtgagtaa tccgtggccc tagagctggc tacgggaact 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 1920

1861 ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 1980

1921 aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagacccagt 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2040

1981 aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc gaacgccata 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2100

2041 agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg tttgcaaagc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2145) ..........

2101 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc 2160

2101 cgctcttcgt ccggtaatag cggccgtacc gccggggtgc ccacgcgtac tagcacgagg 2160

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac 2220

2161 acagcaactc ctgggccgat ccgaccgccc caacggaatg accaatcgtc ttacttagtg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa 2280

2221 gctatgcgct cgcttgcact tcgctgacga cgacgttttg cagacgctgg actcgttgtt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct 2340

2281 gtacttacca gaagccaaag gcacaaagca tttcagacct ttgcgccttc agtcgcggga 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta 2400

2341 cgtggtaata caaggcctag acgtagcgtc ctacgacgac cgatgggaca ccttgtggat 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca 2460

2401 gtagacataa ttgcttcgcg accgtaactg ggactcacta aaaagagacc agggcggcgt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag 2520

2461 aggtatggcg gtcaacaaat gggagtgttg caaggtcatt ggcccgtaca agtagtagtc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa 2580

2521 attgggcata gcactcgtag gagagagcaa agtagccata gtaatggggg tacttgtctt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc 2640

2581 tagggggaat gtgcctccgt agtcactggt ttgtcctttt ttggcgggaa ttgtaccggg 2640

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2641 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg 2700

2641 cgaaatagtc ttcggtctgt aattgcgaag acctctttga gttgctcgac ctgcgcctac 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc 2760

2701 ttgtccgtct gtagacactt agcgaagtgc tggtgcgact actcgaaatg gcgtcgacgg 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 2820

2761 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 2820

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 2880

2821 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 2940

2881 aaccgcccac agccccgcgt cggtactggg tcagtgcatc gctatcgcct cacatatgac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa 3000

2941 cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtggtatata cgccacactt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 3060

3001 tatggcgtgt ctacgcattc ctcttttatg gcgtagtccg cgagaaggcg aaggagcgag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3120

3061 tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3180

3121 cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3240

3181 gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3300

3241 gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3360

3301 gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3420

3361 gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaagagta 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3480

3421 tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga cccgacacac 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3540

3481 gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc agaactcagg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3600

3541 ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3660

3601 cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3720

3661 tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3780

3721 ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3840

3781 gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa caataaaact 3900

3841 agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactt gttattttga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 3960

3901 cagacgaatg tatttgtcat tatgttcccc acaatactcg gtataagttg ccctttgcag 3960

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... ..........

3961 ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 4020

3961 aacgagatcc ggcgctaatt taaggttgta cctacgacta aatataccca tatttacccg 4020

.......... ......... ORF 2 KanR2 ......... .......... ..........

NruI(4023) .......... .......... ClaI(4057) .......... ..........

4021 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc 4080

4021 agcgctatta cagcccgtta gtccacgctg ttagatagct aacataccct tcgggctacg 4080

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 4140

4081 cggtctcaac aaagactttg taccgtttcc atcgcaacgg ttactacaat gtctactcta 4140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 4200

4141 ccagtctgat ttgaccgact gccttaaata cggagaaggc tggtagttcg taaaataggc 4200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4240) .......... ..........

4201 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt 4260

4201 atgaggacta ctacgtacca atgagtggtg acgctagggg cccttttgtc gtaaggtcca 4260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 4320

4261 taatcttctt ataggactaa gtccactttt ataacaacta cgcgaccgtc acaaggacgc 4320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 4380

4321 ggccaacgta agctaaggac aaacattaac aggaaaattg tcgctagcgc ataaagcaga 4380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 4440

4381 gcgagtccgc gttagtgctt acttattgcc aaaccaacta cgctcactaa aactactgct 4440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 4500

4441 cgcattaccg accggacaac ttgttcagac ctttctttac gtatttgaaa acggtaagag 4500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 4560

4501 tggcctaagt cagcagtgag taccactaaa gagtgaacta ttggaataaa aactgctccc 4560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 4620

4561 ctttaattat ccaacataac tacaacctgc tcagccttag cgtctggcta tggtcctaga 4620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 4680

4621 acggtaggat accttgacgg agccactcaa aagaggaagt aatgtctttg ccgaaaaagt 4680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 4740

4681 ttttatacca taactattag gactatactt atttaacgtc aaagtaaact acgagctact 4740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag aaaaataaac 4800

4741 caaaaagatt cttaattaag tactcgccta tgtataaact tacataaatc tttttatttg 4800

ORF 2 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata 4860

4801 tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actttaacat ttgcaattat 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 4920

4861 aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg gttatccggc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 4980

4921 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5040

4981 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 5040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 5100

5041 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca aaaaacccca 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 5160

5101 gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa tctcgaactg 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5220

5161 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5280

5221 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgccgctaca gggcgcgtcc cattcgcca. .......... .......... .......... 5309

5281 gcggcgatgt cccgcgcagg gtaagcggt. .......... .......... .......... 5309

.......... .......... .......... .......... .......... ..........