Sequence of pET-24_c

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Sequence of pET-24_c_(+)

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Sequence of pET-24 c (+) : 5308 BP; 1260 A; 1373 C; 1421 G; 1254 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ NheI(229) ..........

181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 240

181 gctgcctcga gcttaagcct aggctgggta aacgacaggt ggtcagtacg atcggtatac 240

.......... .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... XbaI(274) .......... ..........

241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 300

241 atatagagga agaatttcaa tttgttttaa taaagatctc cccttaacaa taggcgagtg 300

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... BglII(340) .......... ..........

301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 360

301 ttaaggggat atcactcagc ataattaaag cgccctagct ctagagctag gagatgcggc 360

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 420

361 ctgcgtagca ccggccgtag tggccgcggt gtccacgcca acgaccgcgg atatagcggc 420

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 480

421 tgtagtggct accccttcta gcccgagcgg tgaagcccga gtactcgcga acaaagccgc 480

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 540

481 acccatacca ccgtccgggg caccggcccc ctgacaaccc gcggtagagg aacgtacgtg 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 600

541 gtaaggaacg ccgccgccac gagttgccgg agttggatga tgacccgacg aaggattacg 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 660

601 tcctcagcgt attccctctc gcagctctag ggcctgtggt agcttaccgc gttttggaaa 660

tet(564-300) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 720

661 gcgccatacc gtactatcgc gggccttctc tcagttaagt cccaccactt acactttggt 720

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 780

721 cattgcaata tgctacagcg tctcatacgg ccacagagaa tagtctggca aagggcgcac 780

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 840

781 cacttggtcc ggtcggtgca aagacgcttt tgcgcccttt ttcaccttcg ccgctaccgc 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 900

841 ctcgacttaa tgtaagggtt ggcgcaccgt gttgttgacc gcccgtttgt cagcaacgac 900

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 960

901 taaccgcaac ggtggaggtc agaccgggac gtgcgcggca gcgtttaaca gcgccgctaa 960

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1020

961 tttagagcgc ggctagttga cccacggtcg caccaccaca gctaccatct tgcttcgccg 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1076)

1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1080

1021 cagcttcgga catttcgccg ccacgtgtta gaagagcgcg ttgcgcagtc acccgactag 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1140

1081 taattgatag gcgacctact ggtcctacgg taacgacacc ttcgacggac gtgattacaa 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1200

1141 ggccgcaata aagaactaca gagactggtc tgtgggtagt tgtcataata aaagagggta 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1260

1201 cttctgccat gcgctgaccc gcacctcgta gaccagcgta acccagtggt cgtttagcgc 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1273) .......... .......... .......... ..........

1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 1320

1261 gacaatcgcc cgggtaattc aagacagagc cgcgcagacg cagaccgacc gaccgtattt 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 1380

1321 atagagtgag cgttagttta agtcggctat cgccttgccc ttccgctgac ctcacggtac 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 1440

1381 aggccaaaag ttgtttggta cgtttacgac ttactcccgt agcaagggtg acgctacgac 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 1500

1441 caacggttgc tagtctaccg cgacccgcgt tacgcgcggt aatggctcag gcccgacgcg 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 1560

1501 caaccacgcc tatagagcca tcaccctatg ctgctatggc ttctgtcgag tacaatatag 1560

.......... .......... lacI ORF 1 .......... .......... ..........

HpaI(1568) .......... .......... .......... .......... ..........

1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 1620

1561 ggcggcaatt ggtggtagtt tgtcctaaaa gcggacgacc ccgtttggtc gcacctggcg 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 1680

1621 aacgacgttg agagagtccc ggtccgccac ttcccgttag tcgacaacgg gcagagtgac 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 1740

1681 cacttttctt tttggtggga ccgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1800

1741 ctaagtaatt acgtcgaccg tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 1860

1801 gcgttaatta cattcaatcg agtgagtaat ccgtggccct agagctggct acgggaactc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 1920

1861 tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg tactgatagc agcggcgtga 1920

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 1980

1921 atactgacag aagaaatagt acgttgagca tcctgtccac ggccgtcgcg agacccagta 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 2040

1981 aaagccgctc ctggcgaaag cgacctcgcg ctgctactag ccggacagcg aacgccataa 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 2100

2041 gccttagaac gtgcgggagc gagttcggaa gcagtgacca gggcggtggt ttgcaaagcc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2144) ..........

2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 2160

2101 gctcttcgtc cggtaatagc ggccgtaccg ccggggtgcc cacgcgtact agcacgagga 2160

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 2220

2161 cagcaactcc tgggccgatc cgaccgcccc aacggaatga ccaatcgtct tacttagtgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 2280

2221 ctatgcgctc gcttgcactt cgctgacgac gacgttttgc agacgctgga ctcgttgttg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 2340

2281 tacttaccag aagccaaagg cacaaagcat ttcagacctt tgcgccttca gtcgcgggac 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 2400

2341 gtggtaatac aaggcctaga cgtagcgtcc tacgacgacc gatgggacac cttgtggatg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 2460

2401 tagacataat tgcttcgcga ccgtaactgg gactcactaa aaagagacca gggcggcgta 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 2520

2461 ggtatggcgg tcaacaaatg ggagtgttgc aaggtcattg gcccgtacaa gtagtagtca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 2580

2521 ttgggcatag cactcgtagg agagagcaaa gtagccatag taatgggggt acttgtcttt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 2640

2581 agggggaatg tgcctccgta gtcactggtt tgtccttttt tggcgggaat tgtaccgggc 2640

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 2700

2641 gaaatagtct tcggtctgta attgcgaaga cctctttgag ttgctcgacc tgcgcctact 2700

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 2760

2701 tgtccgtctg tagacactta gcgaagtgct ggtgcgacta ctcgaaatgg cgtcgacgga 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2820

2761 gcgcgcaaag ccactactgc cacttttgga gactgtgtac gtcgagggcc tctgccagtg 2820

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2880

2821 tcgaacagac attcgcctac ggccctcgtc tgttcgggca gtcccgcgca gtcgcccaca 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 2940

2881 accgcccaca gccccgcgtc ggtactgggt cagtgcatcg ctatcgcctc acatatgacc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 3000

2941 gaattgatac gccgtagtct cgtctaacat gactctcacg tggtatatac gccacacttt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 3060

3001 atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc gagaaggcga aggagcgagt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3120

3061 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3180

3121 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3240

3181 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 3240

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3300

3241 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3360

3301 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3420

3361 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3480

3421 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3540

3481 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3600

3541 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3660

3601 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3720

3661 cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3780

3721 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3840

3781 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg 3900

3841 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtacttg ttattttgac 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 3960

3901 agacgaatgt atttgtcatt atgttcccca caatactcgg tataagttgc cctttgcaga 3960

.......... .......... .......... ......... ORF 1 KanR2 .........

.......... .......... .......... .......... .......... ..........

3961 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 4020

3961 acgagatccg gcgctaattt aaggttgtac ctacgactaa atatacccat atttacccga 4020

.......... ......... ORF 1 KanR2 ......... .......... ..........

NruI(4022) .......... .......... ClaI(4056) .......... ..........

4021 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 4080

4021 gcgctattac agcccgttag tccacgctgt tagatagcta acataccctt cgggctacgc 4080

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 4140

4081 ggtctcaaca aagactttgt accgtttcca tcgcaacggt tactacaatg tctactctac 4140

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 4200

4141 cagtctgatt tgaccgactg ccttaaatac ggagaaggct ggtagttcgt aaaataggca 4200

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4239) .......... ..........

4201 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 4260

4201 tgaggactac tacgtaccaa tgagtggtga cgctaggggc ccttttgtcg taaggtccat 4260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 4320

4261 aatcttctta taggactaag tccactttta taacaactac gcgaccgtca caaggacgcg 4320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 4380

4321 gccaacgtaa gctaaggaca aacattaaca ggaaaattgt cgctagcgca taaagcagag 4380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 4440

4381 cgagtccgcg ttagtgctta cttattgcca aaccaactac gctcactaaa actactgctc 4440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 4500

4441 gcattaccga ccggacaact tgttcagacc tttctttacg tatttgaaaa cggtaagagt 4500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 4560

4501 ggcctaagtc agcagtgagt accactaaag agtgaactat tggaataaaa actgctcccc 4560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 4620

4561 tttaattatc caacataact acaacctgct cagccttagc gtctggctat ggtcctagaa 4620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 4680

4621 cggtaggata ccttgacgga gccactcaaa agaggaagta atgtctttgc cgaaaaagtt 4680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 4740

4681 tttataccat aactattagg actatactta tttaacgtca aagtaaacta cgagctactc 4740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca 4800

4741 aaaaagattc ttaattaagt actcgcctat gtataaactt acataaatct ttttatttgt 4800

ORF 1 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat 4860

4801 ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctttaacatt tgcaattata 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 4920

4861 aaacaatttt aagcgcaatt taaaaacaat ttagtcgagt aaaaaattgg ttatccggct 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 4980

4921 ttagccgttt tagggaatat ttagttttct tatctggctc tatcccaact cacaacaagg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 5040

4981 tcaaaccttg ttctcaggtg ataatttctt gcacctgagg ttgcagtttc ccgctttttg 5040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 5100

5041 gcagatagtc ccgctaccgg gtgatgcact tggtagtggg attagttcaa aaaaccccag 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 5160

5101 ctccacggca tttcgtgatt tagccttggg atttccctcg ggggctaaat ctcgaactgc 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 5220

5161 ccctttcggc cgcttgcacc gctctttcct tcccttcttt cgctttcctc gcccgcgatc 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 5280

5221 ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg tgtgggcggc gcgaattacg 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gccgctacag ggcgcgtccc attcgcca.. .......... .......... .......... 5308

5281 cggcgatgtc ccgcgcaggg taagcggt.. .......... .......... .......... 5308

.......... .......... .......... .......... .......... ..........