Sequence of pET-25b(+)

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Sequence of pET-25b(+)

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Sequence of pET-25b(+) : 5547 BP; 1307 A; 1472 C; 1479 G; 1289 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... ....... T7_term T7_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg < font color="#000000" style="font-size:10px" face="Courier">aaagcccgaa 120

.... T7_Terminal_pri T7_term T7_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gacatcctcg gggtcttccg 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctgtaggagc cccagaaggc 180

T7_term T7_term .... 6xHis .......... .......... ..........

......... NheI7) XhoI(212) NotI(220),EagI(220) HindIII(227)

181 gggcgagttc tggctggcta gcccgtttga tctcgagtgc ggccgcaagc ttgtcgacgg 240

181 cccgctcaag accgaccgat cgggcaaact agagctcacg ccggcgttcg aacagctgcc 240

.......... .......... .......... .......... .......... ..........

SacI(244),EcoRI(246) BamHI(252) .......... .......... ..........

241 agctcgaatt cggatccgaa ttaattccga tatccatggc catcgccggc tgggcagcga 300

241 tcgagcttaa gcctaggctt aattaaggct ataggtaccg gtagcggccg acccgtcgct 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(342) ..........

301 ggagcagcag accagcagca gcggtcggca gcaggtattt catatgtata tctccttctt 360

301 cctcgtcgtc tggtcgtcgt cgccagccgt cgtccataaa gtatacatat agaggaagaa 360

.......... .......... .......... .......... ....... T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

361 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt 420

361 tttcaatttg ttttaataaa gatctcccct taacaatagg cgagtgttaa ggggatatca 420

.......... .......... .......... lacO .......... ..........

.......... .......... BglII(446) .......... .......... ..........

421 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc 480

421 ctcagcataa ttaaagcgcc ctagctctag agctaggaga tgcggcctgc gtagcaccgg 480

T7_pro .......... .......... .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

481 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg 540

481 ccgtagtggc cgcggtgtcc acgccaacga ccgcggatat agcggctgta gt ggctaccc 540

.......... ......... tet(564-300) ....... pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

541 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca 600

541 cttctagccc gagcggtgaa gcccgagtac tcgcgaacaa agccgcaccc ataccaccgt 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg 660

601 ccggggcacc ggccccctga caacccgcgg tagaggaacg tacgtggtaa ggaacgccgc 660

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag 720

661 cgccacgagt tgccggagtt ggatgatgac ccgacgaagg attacgtcct cagcgtattc 720

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg 780

721 cctctcgcag ctctagggcc tgtggtagct taccgcgttt tggaaagcgc cataccgtac 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga 840

781 tatcgcgggc cttctctcag ttaagtccca ccacttacac tttggtcatt gcaatatgct 840

.......... .......... .......... .......... lacI ..........

.......... .......... .......... .......... .......... ..........

841 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag 900

841 acagcgtctc atacggccac agagaatagt ctggcaaagg gcgcaccact tggtccggtc 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat 960

901 ggtgcaaaga cgcttttgcg ccctttttca ccttcgccgc taccgcctcg acttaatgta 960

.......... .......... lacI .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

961 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac 1020

961 agggttggcg caccgtgttg ttgaccgccc gtttgtcagc aacgactaac cgcaacggtg 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga 1080

1021 gaggtcagac cgggacgtgc gcggcagcgt ttaacagcgc cgctaattta gagcgcggct 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa 1140

1081 agttgaccca cggtcgcacc accacagcta ccatcttgct tcgccgcagc ttcggacatt 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... BclI(1182) ..........

1141 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct 1200

1141 tcgccgccac gtgttagaag agcgcgttgc gcagtcaccc gactagtaat tgataggcga 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct 1260

1201 cctactggtc ctacggtaac gacaccttcg acggacgtga ttacaaggcc gcaataaaga 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg 1320

1261 actacagaga ctggtctgtg ggtagttgtc ataataaaag agggtacttc tgccatgcgc 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(1379)

1321 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc 1380

1321 tgacccgcac ctcgtagacc agcgtaaccc agtggtcgtt tagcgcgaca atcgcccggg 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa 1440

1381 taattcaaga cagagccgcg cagacgcaga ccgaccgacc gtatttatag agtgagcgtt 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca 1500

1441 agtttaagtc ggctatcgcc ttgcccttcc gctgacctca cggtacaggc caaaagttgt 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca 1560

1501 ttggtacgtt tacgacttac tcccgtagca agggtgacgc tacgaccaac ggttgctagt 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat 1620

1561 ctaccgcgac ccgcgttacg cgcggtaatg gctcaggccc gacgcgcaac cacgcctata 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(1674)

1621 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 1680

1621 gagccatcac cctatgctgc tatggcttct gtcgagtaca atatagggcg gcaattggtg 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 1740

1681 gtagtttgtc ctaaaagcgg acgaccccgt ttggtcgcac ctggcgaacg acgttgagag 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac 1800

1741 agtcccggtc cgccacttcc cgttagtcga caacgggcag agtgaccact tttctttttg 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 1860

1801 gtgggaccgc gggttatgcg tttggcggag aggggcgcgc aaccggctaa gtaattacgt 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa 1920

1861 cgaccgtgct gtccaaaggg ctgacctttc gcccgtcact cgcgttgcgt taattacatt 1920

.......... lacI ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag 1980

1921 caatcgagtg agtaatccgt ggccctagag ctggctacgg gaactctcgg aagttgggtc 1980

.......... .......... .......... ......... tet(852-576) .........

.......... .......... .......... .......... .......... ..........

1981 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 2040

1981 agtcgaggaa ggccacccgc gccccgtact gatagcagcg gcgtgaatac tgacagaaga 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 2100

2041 aatagtacgt tgagcatcct gtccacggcc gtcgcgagac ccagtaaaag ccgctcctgg 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 2160

2101 cgaaagcgac ctcgcgctgc tactagccgg acagcgaacg ccataagcct tagaacgtgc 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 2220

2161 gggagcgagt tcggaagcag tgaccagggc ggtggtttgc aaagccgctc ttcgtccggt 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttatcgccgg catggcggcc ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 2280

2221 aatagcggcc gtaccgccgg ggtgcccacg cgtactagca cgaggacagc aactcctggg 2280

tet(852-576) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 2340

2281 ccgatccgac cgccccaacg gaatgaccaa tcgtcttact tagtggctat gcgctcgctt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 2400

2341 gcacttcgct gacgacgacg ttttgcagac gctggactcg ttgttgtact taccagaagc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 2460

2401 caaaggcaca aagcatttca gacctttgcg ccttcagtcg cgggacgtgg taatacaagg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga 2520

2461 cctagacgta gcgtcctacg acgaccgatg ggacaccttg tggatgtaga cataattgct 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt 2580

2521 tcgcgaccgt aactgggact cactaaaaag agaccagggc ggcgtaggta tggcggtcaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga 2640

2581 caaatgggag tgttgcaagg tcattggccc gtacaagtag tagtcattgg gcatagcact 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg 2700

2641 cgtaggagag agcaaagtag ccatagtaat gggggtactt gtctttaggg ggaatgtgcc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc 2760

2701 tccgtagtca ctggtttgtc cttttttggc gggaattgta ccgggcgaaa tagtcttcgg 2760

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct 2820

2761 tctgtaattg cgaagacctc tttgagttgc tcgacctgcg cctacttgtc cgtctgtaga 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg 2880

2821 cacttagcga agtgctggtg cgactactcg aaatggcgtc gacggagcgc gcaaagccac 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 2940

2881 tactgccact tttggagact gtgtacgtcg agggcctctg ccagtgtcga acagacattc 2940

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 3000

2941 gcctacggcc ctcgtctgtt cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 3060

3001 cgcgtcggta ctgggtcagt gcatcgctat cgcctcacat atgaccgaat tgatacgccg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc 3120

3061 tagtctcgtc taacatgact ctcacgtggt atatacgcca cactttatgg cgtgtctacg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 3180

3121 cattcctctt ttatggcgta gtccgcgaga aggcgaagga gcgagtgact gagcgacgcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 3240

3181 agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 3300

3241 tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 3360

3301 ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta 3360

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 3420

3361 gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 3480

3421 cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 3540

3481 atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 3600

3541 atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 3660

3601 gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 3720

3661 ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 3780

3721 ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 3840

3781 ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3900

3841 ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3960

3901 tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc 3960

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 4020

3961 ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 4080

4021 taggaaaatt taatttttac ttcaaaattt agttagattt catatatact catttgaacc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 4140

4081 agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca 4140

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 4200

4141 agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 4260

4201 agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 4320

4261 cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 4380

4321 aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... PstI(4407) .......... .......... ..........

4381 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 4440

4381 aacgcgttgc aacaacggta acgacgtccg tagcaccaca gtgcgagcag caaaccatac 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 4500

4441 cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 4560

4501 ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 4620

4561 aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 4680

4621 acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 4740

4681 ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 4800

4741 tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 4860

4801 aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4920

4861 aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4980

4921 tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa 4980

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 5040

4981 atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt 5040

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt 5100

5041 tatccccaag gcgcgtgtaa aggggctttt cacggtggac tttaacattt gcaattataa 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 5160

5101 aacaatttta agcgcaattt aaaaacaatt tagtcgagta aaaaattggt tatccggctt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 5220

5161 tagccgtttt agggaatatt tagttttctt atctggctct atcccaactc acaacaaggt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 5280

5221 caaaccttgt tctcaggtga taatttcttg cacctgaggt tgcagtttcc cgctttttgg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 5340

5281 cagatagtcc cgctaccggg tgatgcactt ggtagtggga ttagttcaaa aaaccccagc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 5400

5341 tccacggcat ttcgtgattt agccttggga tttccctcgg gggctaaatc tcgaactgcc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 5460

5401 cctttcggcc gcttgcaccg ctctttcctt cccttctttc gctttcctcg cccgcgatcc 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 5520

5461 cgcgaccgtt cacatcgcca gtgcgacgcg cattggtggt gtgggcggcg cgaattacgc 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ccgctacagg gcgcgtccca ttcgcca... .......... .......... .......... 5547

5521 ggcgatgtcc cgcgcagggt aagcggt... .......... .......... .......... 5547

.......... .......... .......... .......... .......... ..........