Sequence of pET-28_a

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Sequence of pET-28_a_(+)

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Sequence of pET-28 a (+) : 6781 BP; 1386 A; 1439 C; 1500 G; 1355 T; 1101 other;

.......... .......... .......... .......... .......... ..........

1 genbankseq uencelocus petabpdnas ynaugdefin itionpetaa ccessionke 60

1 cenvtnmseq aengelogas peatvphnts rntachefin iaionpeatt ggessionme 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ywordssour ceorganism otherseque ncesartifi cialsequen cesvectors 120

61 rwoyhssoay geoyctnisk oadeyseqae ngestyaifi gitlseqaen gesbegaoys 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 featureslo cationqual ifierssour ceorganism petamoltyp eotherdnat 180

121 fetaayeslo gtaionqatl ifieyssoay geoyctnisk peatkolarp eoadeyhnta 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... NheI(231)

181 erminatorc omplementl abelttermi natormiscf eaturelabe ltterminal 240

181 eykintaoyg okplekenal tvelaaeyki ntaoykisgf etaayeltve laaeykintl 240

.......... .......... .......... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... NcoI(296)

241 primermisc featurecom plementlab elxhismisc featurecom plementlab 300

241 pyikeykisg fetaayegok plekenaltv elxdiskisg fetaayegok plekenaltv 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 eltgenelea dermiscfea turecomple mentlabelt leadermisc featurecom 360

301 elacenelet heykisgfet aayegokple kenaltvela letheykisg fetaayegok 360

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... .......... BglII(401) ..........

361 plementlab elxpressfw dprimermis cfeatureco mplementla belttransl 420

361 plekenaltv elxpyessfw hpyikeykis gfetaayego kplekenalt velaaytnsl 420

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 enrbsmiscf eaturecomp lementlabe llacopromo tercomplem entlabeltp 480

421 enyvskisgf etaayegokp lekenaltve lltgopyoko aeygokplek enaltvelap 480

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

481 romotermis cfeatureco mplementla belpbrrevb amprimerge necompleme 540

481 yokoaeykis gfetaayego kplekenalt velpvyyebv tkpyikeyce negokpleke 540

pBRrevBam_pri ..... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ntlabeltet genetetmis cfeaturela bellacicds labelorffr ametransla 600

541 naltvelaea ceneaeakis gfetaayelt velltgighs ltveloyffy tkeaytnslt 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tionmaelny ipnrvaqqla gkqslligva tsslalhaps qivaaiksra dqlgasvvvs 660

601 aionktelnr ipnybtqqlt cmqsllicbt assltldtps qibttimsyt hqlctsbbbs 660

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 mversgveac kaavhnllaq rvsgliinyp lddqdaiave aactnvpalf ldvsdqtpin 720

661 kbeyscbetg mttbdnlltq ybscliinrp lhhqhtitbe ttganbptlf lhbshqapin 720

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 siifshedgt rlgvehlval ghqqiallag plssvsarlr lagwhkyltr nqiqpiaere 780

721 siifsdehca ylcbedlbtl cdqqitlltc plssbstyly ltcwdmrlay nqiqpiteye 780

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 gdwsamsgfq qtmqmlnegi vptamlvand qmalgamrai tesglrvgad isvvgyddte 840

781 chwstkscfq qakqklneci bpatklbtnh qktlctkyti aesclybcth isbbcrhhae 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 dsscyipplt tikqdfrllg qtsvdrllql sqgqavkgnq llpvslvkrk ttlapntqta 900

841 hssgrippla aimqhfyllc qasbhyllql sqcqtbmcnq llpbslbmym aaltpnaqat 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 spraladslm qlarqvsrle sgqgenecom plementlab eltetgenet etmiscfeat 960

901 spytlthslk qltyqbsyle scqcenegok plekenaltv elaeacenea eakisgfeta 960

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 urelabelro pmiscfeatu recompleme ntlabelpge xprimerrep origincomp 1020

961 ayeltvelyo pkisgfetaa yegokpleke naltvelpce xpyikeyyep oyicingokp 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 lementlabe lpbrorigin cdslabelka nrgenekanr noteorffra metranslat 1080

1021 lekenaltve lpvyoyicin ghsltvelmt nycenemtny noaeoyffyt keaytnslta 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1137)

1081 ionmshiqre tscsrprlns nmdadlygyk wardnvgqsg atiyrlygkp dapelflkhg 1140

1081 ionksdiqye asgsypylns nkhthlrcrm wtyhnbcqsc tairylrcmp htpelflmdc 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 kgsvandvtd emvrlnwlte fmplptikhf irtpddawll ttaipgktaf qvleeypdsg 1200

1141 mcsbtnhbah ekbylnwlae fkplpaimdf iyaphhtwll aatipcmatf qbleerphsc 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 enivdalavf lrrlhsipvc ncpfnsdrvf rlaqaqsrmn nglvdasdfd derngwpveq 1260

1201 enibhtltbf lyyldsipbg ngpfnshybf yltqtqsykn nclbhtshfh heyncwpbeq 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 vwkemhkllp fspdsvvthg dfsldnlifd egkligcidv grvgiadryq dlailwnclg 1320

1261 bwmekdmllp fsphsbbadc hfslhnlifh ecmlicgihb cybcithyrq hltilwnglc 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1334) .......... .......... .......... ..........

1321 efspslqkrl fqkygidnpd mnklqfhlml deffgenela belkanrgen ekanrrepor 1380

1321 efspslqmyl fqmrcihnph knmlqfdlkl heffcenelt velmtnycen emtnyyepoy 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 igincomple mentlabelf originorig inatccggat atagttcctc ctttcagcaa 1440

1381 icingokple kenaltvelf oyicinoyic intaggccta tatcaaggag gaaagtcgtt 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt 1500

1441 ttttggggag ttctgggcaa atctccgggg ttccccaata cgatcaataa cgagtcgcca 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggcagcagcc aactcagctt cctttcgggc tttgttagca gccggatctc agtggtggtg 1560

1501 ccgtcgtcgg ttgagtcgaa ggaaagcccg aaacaatcgt cggcctagag tcaccaccac 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtggtggtgc tcgagtgcgg ccgcaagctt gtcgacggag ctcgaattcg gatccgcgac 1620

1561 caccaccacg agctcacgcc ggcgttcgaa cagctgcctc gagcttaagc ctaggcgctg 1620

.......... .......... lacI ORF 2 .......... .......... ..........

HpaI(1629) .......... .......... .......... .......... ..........

1621 ccatttgctg tccaccagtc atgctagcca tatggctgcc gcgcggcacc aggccgctgc 1680

1621 ggtaaacgac aggtggtcag tacgatcggt ataccgacgg cgcgccgtgg tccggcgacg 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtgatgatg atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa 1740

1681 acactactac tactactacc gacgacgggt accatataga ggaagaattt caatttgttt 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat 1800

1741 taataaagat ctccccttaa caataggcga gtgttaaggg gatatcactc agcataatta 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg 1860

1801 aagcgcccta gctctagagc taggagatgc ggcctgcgta gcaccggccg tagtggccgc 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc 1920

1861 ggtgtccacg ccaacgaccg cggatatagc ggctgtagtg gctacccctt ctagcccgag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 1980

1921 cggtgaagcc cgagtactcg cgaacaaagc cgcacccata ccaccgtccg gggcaccggc 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg 2040

1981 cccctgacaa cccgcggtag aggaacgtac gtggtaagga acgccgccgc cacgagttgc 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag 2100

2041 cggagttgga tgatgacccg acgaaggatt acgtcctcag cgtattccct ctcgcagctc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa 2160

2101 tagggcctgt ggtagcttac cgcgttttgg aaagcgccat accgtactat cgcgggcctt 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2205) ..........

2161 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 2220

2161 ctctcagtta agtcccacca cttacacttt ggtcattgca atatgctaca gcgtctcata 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 2280

2221 cggccacaga gaatagtctg gcaaagggcg caccacttgg tccggtcggt gcaaagacgc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 2340

2281 ttttgcgccc tttttcacct tcgccgctac cgcctcgact taatgtaagg gttggcgcac 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 2400

2341 cgtgttgttg accgcccgtt tgtcagcaac gactaaccgc aacggtggag gtcagaccgg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 2460

2401 gacgtgcgcg gcagcgttta acagcgccgc taatttagag cgcggctagt tgacccacgg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 2520

2461 tcgcaccacc acagctacca tcttgcttcg ccgcagcttc ggacatttcg ccgccacgtg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 2580

2521 ttagaagagc gcgttgcgca gtcacccgac tagtaattga taggcgacct actggtccta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 2640

2581 cggtaacgac accttcgacg gacgtgatta caaggccgca ataaagaact acagagactg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 2700

2641 gtctgtgggt agttgtcata ataaaagagg gtacttctgc catgcgctga cccgcacctc 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 2760

2701 gtagaccagc gtaacccagt ggtcgtttag cgcgacaatc gcccgggtaa ttcaagacag 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 2820

2761 agccgcgcag acgcagaccg accgaccgta tttatagagt gagcgttagt ttaagtcggc 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 2880

2821 tatcgccttg cccttccgct gacctcacgg tacaggccaa aagttgtttg gtacgtttac 2880

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 2940

2881 gacttactcc cgtagcaagg gtgacgctac gaccaacggt tgctagtcta ccgcgacccg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 3000

2941 cgttacgcgc ggtaatggct caggcccgac gcgcaaccac gcctatagag ccatcaccct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 3060

3001 atgctgctat ggcttctgtc gagtacaata tagggcggca attggtggta gtttgtccta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 3120

3061 aaagcggacg accccgtttg gtcgcacctg gcgaacgacg ttgagagagt cccggtccgc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 3180

3121 cacttcccgt tagtcgacaa cgggcagagt gaccactttt ctttttggtg ggaccgcggg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 3240

3181 ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta attacgtcga ccgtgctgtc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca 3300

3241 caaagggctg acctttcgcc cgtcactcgc gttgcgttaa ttacattcaa tcgagtgagt 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg 3360

3301 aatccgtggc cctagagctg gctacgggaa ctctcggaag ttgggtcagt cgaggaaggc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact 3420

3361 cacccgcgcc ccgtactgat agcagcggcg tgaatactga cagaagaaat agtacgttga 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag 3480

3421 gcatcctgtc cacggccgtc gcgagaccca gtaaaagccg ctcctggcga aagcgacctc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc 3540

3481 gcgctgctac tagccggaca gcgaacgcca taagccttag aacgtgcggg agcgagttcg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat 3600

3541 gaagcagtga ccagggcggt ggtttgcaaa gccgctcttc gtccggtaat agcggccgta 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg 3660

3601 ccgccggggt gcccacgcgt actagcacga ggacagcaac tcctgggccg atccgaccgc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg 3720

3661 cccaacggaa tgaccaatcg tcttacttag tggctatgcg ctcgcttgca cttcgctgac 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc 3780

3721 gacgacgttt tgcagacgct ggactcgttg ttgtacttac cagaagccaa aggcacaaag 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc 3840

3781 catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa tacaaggcct agacgtagcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg 3900

3841 tcctacgacg accgatggga caccttgtgg atgtagacat aattgcttcg cgaccgtaac 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca 3960

3901 tgggactcac taaaaagaga ccagggcggc gtaggtatgg cggtcaacaa atgggagtgt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg 4020

3961 tgcaaggtca ttggcccgta caagtagtag tcattgggca tagcactcgt aggagagagc 4020

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... ..........

4021 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac 4080

4021 aaagtagcca tagtaatggg ggtacttgtc tttaggggga atgtgcctcc gtagtcactg 4080

.......... ......... ORF 2 KanR2 ......... .......... ..........

NruI(4083) .......... .......... ClaI(4117) .......... ..........

4081 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct 4140

4081 gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag tcttcggtct gtaattgcga 4140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca 4200

4141 agacctcttt gagttgctcg acctgcgcct acttgtccgt ctgtagacac ttagcgaagt 4200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa 4260

4201 gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca aagccactac tgccactttt 4260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4300) .......... ..........

4261 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 4320

4261 ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc tacggccctc 4320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 4380

4321 gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc acagccccgc gtcggtactg 4380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt 4440

4381 ggtcagtgca tcgctatcgc ctcacatatg accgaattga tacgccgtag tctcgtctaa 4440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 4500

4441 catgactctc acgtggtata tacgccacac tttatggcgt gtctacgcat tcctctttta 4500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4560

4501 tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg 4560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4620

4561 acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc 4620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4680

4621 tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc 4680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4740

4681 ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg 4740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4800

4741 cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac 4800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4860

4801 cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga 4860

ORF 2 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4920

4861 aagagggaag cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4980

4921 acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5040

4981 cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5100

5041 accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5160

5101 agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5220

5161 acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5280

5221 ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5340

5281 gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gttaagggat tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg 5400

5341 caattcccta aaaccagtac ttgttatttt gacagacgaa tgtatttgtc attatgttcc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggtgttatga gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac 5460

5401 ccacaatact cggtataagt tgccctttgc agaacgagat ccggcgctaa tttaaggttg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 5520

5461 tacctacgac taaatatacc catatttacc cgagcgctat tacagcccgt tagtccacgc 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 5580

5521 tgttagatag ctaacatacc cttcgggcta cgcggtctca acaaagactt tgtaccgttt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 5640

5581 ccatcgcaac ggttactaca atgtctactc taccagtctg atttgaccga ctgccttaaa 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 5700

5641 tacggagaag gctggtagtt cgtaaaatag gcatgaggac tactacgtac caatgagtgg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 5760

5701 tgacgctagg ggcccttttg tcgtaaggtc cataatcttc ttataggact aagtccactt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 5820

5761 ttataacaac tacgcgaccg tcacaaggac gcggccaacg taagctaagg acaaacatta 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 5880

5821 acaggaaaat tgtcgctagc gcataaagca gagcgagtcc gcgttagtgc ttacttattg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 5940

5881 ccaaaccaac tacgctcact aaaactactg ctcgcattac cgaccggaca acttgttcag 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat 6000

5941 acctttcttt acgtatttga aaacggtaag agtggcctaa gtcagcagtg agtaccacta 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 6060

6001 aagagtgaac tattggaata aaaactgctc ccctttaatt atccaacata actacaacct 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 6120

6061 gctcagcctt agcgtctggc tatggtccta gaacggtagg ataccttgac ggagccactc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 6180

6121 aaaagaggaa gtaatgtctt tgccgaaaaa gtttttatac cataactatt aggactatac 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aataaattgc agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg 6240

6181 ttatttaacg tcaaagtaaa ctacgagcta ctcaaaaaga ttcttaatta agtactcgcc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6300

6241 tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt gtaaaggggc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aaaagtgcca cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg 6360

6301 ttttcacggt ggactttaac atttgcaatt ataaaacaat tttaagcgca atttaaaaac 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 6420

6361 aatttagtcg agtaaaaaat tggttatccg gctttagccg ttttagggaa tatttagttt 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 6480

6421 tcttatctgg ctctatccca actcacaaca aggtcaaacc ttgttctcag gtgataattt 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 6540

6481 cttgcacctg aggttgcagt ttcccgcttt ttggcagata gtcccgctac cgggtgatgc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 6600

6541 acttggtagt gggattagtt caaaaaaccc cagctccacg gcatttcgtg atttagcctt 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 6660

6601 gggatttccc tcgggggcta aatctcgaac tgcccctttc ggccgcttgc accgctcttt 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 6720

6661 ccttcccttc tttcgctttc ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc 6780

6721 cgcgcattgg tggtgtgggc ggcgcgaatt acgcggcgat gtcccgcgca gggtaagcgg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 a......... .......... .......... .......... .......... .......... 6781

6781 t......... .......... .......... .......... .......... .......... 6781

.......... .......... .......... .......... .......... ..........