Sequence of pET-28_b

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Sequence of pET-28_b_(+)

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Sequence of pET-28 b (+) : 5368 BP; 1268 A; 1391 C; 1443 G; 1266 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ NheI(230) ..........

181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 240

181 gctgcctcga gcttaagcct agggctgggt aaacgacagg tggtcagtac gatcggtata 240

.......... .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... NcoI(295)

241 ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg 300

241 ccgacggcgc gccgtggtcc ggcgacgaca ctactactac tactaccgac gacgggtacc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 360

301 atatagagga agaatttcaa tttgttttaa taaagatctc cccttaacaa taggcgagtg 360

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... BglII(400) .......... ..........

361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 420

361 ttaaggggat atcactcagc ataattaaag cgccctagct ctagagctag gagatgcggc 420

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 480

421 ctgcgtagca ccggccgtag tggccgcggt gtccacgcca acgaccgcgg atatagcggc 480

.......... ......... tet(564-300) ......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

481 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 540

481 tgtagtggct accccttcta gcccgagcgg tgaagcccga gtactcgcga acaaagccgc 540

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 600

541 acccatacca ccgtccgggg caccggcccc ctgacaaccc gcggtagagg aacgtacgtg 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 660

601 gtaaggaacg ccgccgccac gagttgccgg agttggatga tgacccgacg aaggattacg 660

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 720

661 tcctcagcgt attccctctc gcagctctag ggcctgtggt agcttaccgc gttttggaaa 720

tet(564-300) ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 780

721 gcgccatacc gtactatcgc gggccttctc tcagttaagt cccaccactt acactttggt 780

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 840

781 cattgcaata tgctacagcg tctcatacgg ccacagagaa tagtctggca aagggcgcac 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 900

841 cacttggtcc ggtcggtgca aagacgcttt tgcgcccttt ttcaccttcg ccgctaccgc 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 960

901 ctcgacttaa tgtaagggtt ggcgcaccgt gttgttgacc gcccgtttgt cagcaacgac 960

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 1020

961 taaccgcaac ggtggaggtc agaccgggac gtgcgcggca gcgtttaaca gcgccgctaa 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1080

1021 tttagagcgc ggctagttga cccacggtcg caccaccaca gctaccatct tgcttcgccg 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1136)

1081 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1140

1081 cagcttcgga catttcgccg ccacgtgtta gaagagcgcg ttgcgcagtc acccgactag 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1200

1141 taattgatag gcgacctact ggtcctacgg taacgacacc ttcgacggac gtgattacaa 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1260

1201 ggccgcaata aagaactaca gagactggtc tgtgggtagt tgtcataata aaagagggta 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1320

1261 cttctgccat gcgctgaccc gcacctcgta gaccagcgta acccagtggt cgtttagcgc 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1333) .......... .......... .......... ..........

1321 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 1380

1321 gacaatcgcc cgggtaattc aagacagagc cgcgcagacg cagaccgacc gaccgtattt 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 1440

1381 atagagtgag cgttagttta agtcggctat cgccttgccc ttccgctgac ctcacggtac 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 1500

1441 aggccaaaag ttgtttggta cgtttacgac ttactcccgt agcaagggtg acgctacgac 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 1560

1501 caacggttgc tagtctaccg cgacccgcgt tacgcgcggt aatggctcag gcccgacgcg 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 1620

1561 caaccacgcc tatagagcca tcaccctatg ctgctatggc ttctgtcgag tacaatatag 1620

.......... .......... lacI ORF 1 .......... .......... ..........

HpaI(1628) .......... .......... .......... .......... ..........

1621 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 1680

1621 ggcggcaatt ggtggtagtt tgtcctaaaa gcggacgacc ccgtttggtc gcacctggcg 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 1740

1681 aacgacgttg agagagtccc ggtccgccac ttcccgttag tcgacaacgg gcagagtgac 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 1800

1741 cacttttctt tttggtggga ccgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1860

1801 ctaagtaatt acgtcgaccg tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 1920

1861 gcgttaatta cattcaatcg agtgagtaat ccgtggccct agagctggct acgggaactc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 1980

1921 tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg tactgatagc agcggcgtga 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 2040

1981 atactgacag aagaaatagt acgttgagca tcctgtccac ggccgtcgcg agacccagta 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 2100

2041 aaagccgctc ctggcgaaag cgacctcgcg ctgctactag ccggacagcg aacgccataa 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 2160

2101 gccttagaac gtgcgggagc gagttcggaa gcagtgacca gggcggtggt ttgcaaagcc 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2204) ..........

2161 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 2220

2161 gctcttcgtc cggtaatagc ggccgtaccg ccggggtgcc cacgcgtact agcacgagga 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 2280

2221 cagcaactcc tgggccgatc cgaccgcccc aacggaatga ccaatcgtct tacttagtgg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 2340

2281 ctatgcgctc gcttgcactt cgctgacgac gacgttttgc agacgctgga ctcgttgttg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 2400

2341 tacttaccag aagccaaagg cacaaagcat ttcagacctt tgcgccttca gtcgcgggac 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 2460

2401 gtggtaatac aaggcctaga cgtagcgtcc tacgacgacc gatgggacac cttgtggatg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 2520

2461 tagacataat tgcttcgcga ccgtaactgg gactcactaa aaagagacca gggcggcgta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 2580

2521 ggtatggcgg tcaacaaatg ggagtgttgc aaggtcattg gcccgtacaa gtagtagtca 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 2640

2581 ttgggcatag cactcgtagg agagagcaaa gtagccatag taatgggggt acttgtcttt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 2700

2641 agggggaatg tgcctccgta gtcactggtt tgtccttttt tggcgggaat tgtaccgggc 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 2760

2701 gaaatagtct tcggtctgta attgcgaaga cctctttgag ttgctcgacc tgcgcctact 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 2820

2761 tgtccgtctg tagacactta gcgaagtgct ggtgcgacta ctcgaaatgg cgtcgacgga 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2880

2821 gcgcgcaaag ccactactgc cacttttgga gactgtgtac gtcgagggcc tctgccagtg 2880

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2940

2881 tcgaacagac attcgcctac ggccctcgtc tgttcgggca gtcccgcgca gtcgcccaca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 3000

2941 accgcccaca gccccgcgtc ggtactgggt cagtgcatcg ctatcgcctc acatatgacc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 3060

3001 gaattgatac gccgtagtct cgtctaacat gactctcacg tggtatatac gccacacttt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 3120

3061 atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc gagaaggcga aggagcgagt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3180

3121 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3240

3181 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3300

3241 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3360

3301 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3420

3361 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3480

3421 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3540

3481 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3600

3541 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3660

3601 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3720

3661 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3780

3721 cttcctgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3840

3781 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3900

3841 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg 3960

3901 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtacttg ttattttgac 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 4020

3961 agacgaatgt atttgtcatt atgttcccca caatactcgg tataagttgc cctttgcaga 4020

.......... .......... .......... ......... ORF 1 KanR2 .........

.......... .......... .......... .......... .......... ..........

4021 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 4080

4021 acgagatccg gcgctaattt aaggttgtac ctacgactaa atatacccat atttacccga 4080

.......... ......... ORF 1 KanR2 ......... .......... ..........

NruI(4082) .......... .......... ClaI(4116) .......... ..........

4081 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 4140

4081 gcgctattac agcccgttag tccacgctgt tagatagcta acataccctt cgggctacgc 4140

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 4200

4141 ggtctcaaca aagactttgt accgtttcca tcgcaacggt tactacaatg tctactctac 4200

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 4260

4201 cagtctgatt tgaccgactg ccttaaatac ggagaaggct ggtagttcgt aaaataggca 4260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4299) .......... ..........

4261 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 4320

4261 tgaggactac tacgtaccaa tgagtggtga cgctaggggc ccttttgtcg taaggtccat 4320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 4380

4321 aatcttctta taggactaag tccactttta taacaactac gcgaccgtca caaggacgcg 4380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 4440

4381 gccaacgtaa gctaaggaca aacattaaca ggaaaattgt cgctagcgca taaagcagag 4440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 4500

4441 cgagtccgcg ttagtgctta cttattgcca aaccaactac gctcactaaa actactgctc 4500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 4560

4501 gcattaccga ccggacaact tgttcagacc tttctttacg tatttgaaaa cggtaagagt 4560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 4620

4561 ggcctaagtc agcagtgagt accactaaag agtgaactat tggaataaaa actgctcccc 4620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 4680

4621 tttaattatc caacataact acaacctgct cagccttagc gtctggctat ggtcctagaa 4680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 4740

4681 cggtaggata ccttgacgga gccactcaaa agaggaagta atgtctttgc cgaaaaagtt 4740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 4800

4741 tttataccat aactattagg actatactta tttaacgtca aagtaaacta cgagctactc 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca 4860

4801 aaaaagattc ttaattaagt actcgcctat gtataaactt acataaatct ttttatttgt 4860

ORF 1 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat 4920

4861 ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctttaacatt tgcaattata 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 4980

4921 aaacaatttt aagcgcaatt taaaaacaat ttagtcgagt aaaaaattgg ttatccggct 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 5040

4981 ttagccgttt tagggaatat ttagttttct tatctggctc tatcccaact cacaacaagg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 5100

5041 tcaaaccttg ttctcaggtg ataatttctt gcacctgagg ttgcagtttc ccgctttttg 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 5160

5101 gcagatagtc ccgctaccgg gtgatgcact tggtagtggg attagttcaa aaaaccccag 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 5220

5161 ctccacggca tttcgtgatt tagccttggg atttccctcg ggggctaaat ctcgaactgc 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 5280

5221 ccctttcggc cgcttgcacc gctctttcct tcccttcttt cgctttcctc gcccgcgatc 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 5340

5281 ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg tgtgggcggc gcgaattacg 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gccgctacag ggcgcgtccc attcgcca.. .......... .......... .......... 5368

5341 cggcgatgtc ccgcgcaggg taagcggt.. .......... .......... .......... 5368

.......... .......... .......... .......... .......... ..........