;LOCUS pET-28 c (+) 5367 bp DNA SYN 31-Aug-2007 DEFINITION pET-28 c (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5367 /organism="pET-28 c (+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(205..237) /label="T7_gene10_leader" misc_feature complement(205..237) /label="T7_leader" misc_feature complement(220..238) /label="Xpress_fwd_primer" misc_feature complement(305..321) /label="T7_transl_en_RBS" misc_feature complement(339..366) /label="lacO" promoter complement(366..384) /label="T7_promoter" misc_feature complement(468..487) /label="pBRrevBam_primer" gene complement(416..679) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 762..1853 /label="lacI" CDS 894..1853 /label="ORF frame 3" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(1912..2187) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2662..2853 /label="ROP" misc_feature complement(2847..2869) /label="pGEX_3_primer" rep_origin complement(3268..3887) /label="pBR322_origin" CDS 3993..4808 /label="KanR2" /gene="KanR2" /note="ORF frame 3" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" gene 3993..4808 /label="KanR2" /gene="KanR2" rep_origin complement(5033..5339) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt 301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 361 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg 421 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 481 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 541 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 601 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 661 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc 721 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 781 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 841 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 901 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 961 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 1021 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 1081 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 1141 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 1201 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 1261 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 1321 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 1381 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 1441 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 1501 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 1561 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 1621 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 1681 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 1741 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 1801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 1861 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga 1921 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 1981 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 2041 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 2101 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 2161 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg 2221 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 2281 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 2341 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 2401 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca 2461 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 2521 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 2581 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 2641 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 2701 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 2761 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc 2821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2941 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 3001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat 3061 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 3121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3721 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt 3961 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt 4021 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 4081 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 4141 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 4201 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 4261 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat 4321 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 4381 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 4441 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 4501 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 4561 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 4621 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 4681 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 4741 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 4801 ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt 4921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 4981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 5041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 5101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 5161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 5221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 5281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 5341 ccgctacagg gcgcgtccca ttcgcca // Reverse complement: tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttg ccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctcta aatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgt tccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattgg ttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattct tagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccg tttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactc gtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgact gaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaa atcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggac aattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatat tcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatg cttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctac ctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgaca ttatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccg ttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaa cgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgt aatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttc cgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaag aactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggaga aaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggta tctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctat ggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgtta tcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcag cgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgca tatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactg ggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttaca gacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcgg taaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccag aagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgt aagggggatttctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaa catgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcaggg tcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaaca taatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcag gtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaacc ccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcct gcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagc gacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctac gagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggt tgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactg cccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtat tgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagt tgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatga gctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattg cgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttga aaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagcc agccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagat gctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaat aacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccact gacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgc tggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggca acgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgc ttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccgg catactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgcc ataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagc agcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtccc ccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatc ggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagagga tcgagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataa ttttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcg cggcagccatatggctagcatgactggtggacagcaaatgggtcggatccgaattcgagctccgtcgacaagcttgcggc cgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgcca ccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata tccggat pET-28 c (+) "Times New RomanCT7_term"Times New RomanCEWT7_Terminal_pri"Times New RomanCɆ6xHis"Times New RomanT7_gene10_leader"Times New RomanC T7_leader"Times New RomanCXpress_fwd_pri"Times New Roman*CɆ1AT7_transl_en_RBS"Times New RomanSnlacO"Times New RomanCnT7_pro"Times New RomanCɆ tet(564-300)"Times New RomanC pBRrevBam_pri"Times New Roman=lacI"Times New RomanC~=ORF 3"Times New RomanCx tet(852-576)"Times New RomanCɆf % ROP"Times New Roman 5  pGEX_3_pri"Times New RomanC / pBR322_origin"Times New RomanCORF 3"Times New RomanCɆKanR2"Times New Roman f1_origin"Times New Roman rXhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),NheI(229),NdeI(236),NcoI(294)"Times New Roman cF XbaI(333),BglII(399)"Times New RomanMAA"22o BclI"Times New Romano ApaI"Times New Roman4 EcoRV(1571),HpaI(1627)"Times New Roman# FspI"Times New RomanFKPP NruI(4081),ClaI(4115)"Times New Roman SmaI"Times New Roman~w test"Times New RomanaS J test"Times New Roman#P V[[