Sequence of pET-29_a

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Sequence of pET-29_a_(+)

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Sequence of pET-29 a (+) : 5371 BP; 1272 A; 1387 C; 1443 G; 1269 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206) NcoI(212) ........

181 cgacggagct cgaattcgga tccgatatca gccatggaac cgcgtggcac cagggtaccc 240

181 gctgcctcga gcttaagcct aggctatagt cggtaccttg gcgcaccgtg gtcccatggg 240

.......... .......... .......... .......... .......... ..........

BglII(241) .......... BstBI(268) .......... .......... NdeI(295)

241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatatgt 300

241 tctagacccg acaggtacac gaccgcaagc ttaaatcgtc gtcgccaaag aaagtataca 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(333) .......... ..........

301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 360

301 tatagaggaa gaatttcaat ttgttttaat aaagatctcc ccttaacaat aggcgagtgt 360

....... T7_transl_en_RBS ORF 3 ....... lacO ..........

.......... .......... .......... .......... .......... ..........

361 attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg 420

361 taaggggata tcactcagca taattaaagc gccctagctc tagctagagc taggagatgc 420

.......... T7_pro ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 480

421 ggcctgcgta gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc 480

.......... ...... ORF 3 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

481 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 540

481 ggctgtagtg gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc 540

pBRrevBam_pri .. ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 600

541 cgcacccata ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac 600

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 660

601 gtggtaagga acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt 660

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc 720

661 acgtcctcag cgtattccct ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg 720

......... tet(564-300) ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 780

721 aaagcgccat accgtactat cgcgggcctt ctctcagtta agtcccacca cttacacttt 780

.......... .......... ORF 3 .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 840

781 ggtcattgca atatgctaca gcgtctcata cggccacaga gaatagtctg gcaaagggcg 840

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 900

841 caccacttgg tccggtcggt gcaaagacgc ttttgcgccc tttttcacct tcgccgctac 900

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>tggcgggcaa
901 gcggagctga attacattcc caaccgcgtg gcacaacaac acagtcgttg 960

901 cgcctcgact taatgtaagg gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac 960

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>cgtcgcaaat
961 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc tgtcgcggcg 1020

961 gactaaccgc aacggtggag gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc 1020

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 1080

1021 taatttagag cgcggctagt tgacccacgg tcgcaccacc acagctacca tcttgcttcg 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1139)

1081 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 1140

1081 ccgcagcttc ggacatttcg ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 1200

1141 tagtaattga taggcgacct actggtccta cggtaacgac accttcgacg gacgtgatta 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 1260

1201 caaggccgca ataaagaact acagagactg gtctgtgggt agttgtcata ataaaagagg 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 1320

1261 gtacttctgc catgcgctga cccgcacctc gtagaccagc gtaacccagt ggtcgtttag 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1336) .......... .......... .......... ..........

1321 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 1380

1321 cgcgacaatc gcccgggtaa ttcaagacag agccgcgcag acgcagaccg accgaccgta 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 1440

1381 tttatagagt gagcgttagt ttaagtcggc tatcgccttg cccttccgct gacctcacgg 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 1500

1441 tacaggccaa aagttgtttg gtacgtttac gacttactcc cgtagcaagg gtgacgctac 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 1560

1501 gaccaacggt tgctagtcta ccgcgacccg cgttacgcgc ggtaatggct caggcccgac 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 1620

1561 gcgcaaccac gcctgtagag ccatcaccct atgctgctat ggcttctgtc gagtacaata 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... HpaI(1631) .......... .......... .......... ..........

1621 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 1680

1621 tagggcggca attggtggta gtttgtccta aaagcggacg accccgtttg gtcgcacctg 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 1740

1681 gcgaacgacg ttgagagagt cccggtccgc cacttcccgt tagtcgacaa cgggcagagt 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 1800

1741 gaccactttt ctttttggtg ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1860

1801 cggctaagta attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt 1920

1861 gttgcgttaa ttacattcaa tcgagtgagt aatccgtggc cctagagctg gctacgggaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 1980

1921 ctctcggaag ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 2040

1981 tgaatactga cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 2100

2041 gtaaaagccg ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 2160

2101 taagccttag aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2207) ..........

2161 cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct 2220

2161 gccgctcttc gtccggtaat agcggccgta ccgccggggt gcccacgcgt actagcacga 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc 2280

2221 ggacagcaac tcctgggccg atccgaccgc cccaacggaa tgaccaatcg tcttacttag 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac 2340

2281 tggctatgcg ctcgcttgca cttcgctgac gacgacgttt tgcagacgct ggactcgttg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc 2400

2341 ttgtacttac cagaagccaa aggcacaaag catttcagac ctttgcgcct tcagtcgcgg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc 2460

2401 gacgtggtaa tacaaggcct agacgtagcg tcctacgacg accgatggga caccttgtgg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg 2520

2461 atgtagacat aattgcttcg cgaccgtaac tgggactcac taaaaagaga ccagggcggc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc 2580

2521 gtaggtatgg cggtcaacaa atgggagtgt tgcaaggtca ttggcccgta caagtagtag 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag 2640

2581 tcattgggca tagcactcgt aggagagagc aaagtagcca tagtaatggg ggtacttgtc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc 2700

2641 tttaggggga atgtgcctcc gtagtcactg gtttgtcctt ttttggcggg aattgtaccg 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga 2760

2701 ggcgaaatag tcttcggtct gtaattgcga agacctcttt gagttgctcg acctgcgcct 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg 2820

2761 acttgtccgt ctgtagacac ttagcgaagt gctggtgcga ctactcgaaa tggcgtcgac 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 2880

2821 ggagcgcgca aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca 2880

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2881 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 2940

2881 gtgtcgaaca gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac 3000

2941 acaaccgccc acagccccgc gtcggtactg ggtcagtgca tcgctatcgc ctcacatatg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg 3060

3001 accgaattga tacgccgtag tctcgtctaa catgactctc acgtggtata tacgccacac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 3120

3061 tttatggcgt gtctacgcat tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3180

3121 agtgactgag cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3240

3181 gccattatgc caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3300

3241 cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3360

3301 cggggggact gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3420

3361 ctgatatttc tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3480

3421 gggacggcga atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3540

3481 tatcgagtgc gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3600

3541 acgtgcttgg ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3660

3601 ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3720

3661 ctcgctccat acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3780

3721 gatcttcctg tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3840

3781 aaccatcgag aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3900

3841 tcgtcgtcta atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa 3960

3901 ccagactgcg agtcaccttg cttttgagtg caattcccta aaaccagtac ttgttatttt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg 4020

3961 gacagacgaa tgtatttgtc attatgttcc ccacaatact cggtataagt tgccctttgc 4020

.......... .......... .......... ......... ORF 1 KanR2 .........

.......... .......... .......... .......... .......... ..........

4021 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 4080

4021 agaacgagat ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc 4080

.......... ......... ORF 1 KanR2 ......... .......... ..........

NruI(4085) .......... .......... .......... .......... ..........

4081 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat 4140

4081 cgagcgctat tacagcccgt tagtccacgc tgttagatag ctaacatacc cttcgggcta 4140

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 4200

4141 cgcggtctca acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc 4200

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 4260

4201 taccagtctg atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag 4260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... SmaI(4302) ..........

4261 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag 4320

4261 gcatgaggac tactacgtac caatgagtgg tgacgctagg ggcccttttg tcgtaaggtc 4320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 4380

4321 cataatcttc ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac 4380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 4440

4381 gcggccaacg taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca 4440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 4500

4441 gagcgagtcc gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg 4500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 4560

4501 ctcgcattac cgaccggaca acttgttcag acctttcttt acgtatttga aaacggtaag 4560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 4620

4561 agtggcctaa gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc 4620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 4680

4621 ccctttaatt atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta 4680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 4740

4681 gaacggtagg ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa 4740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 4800

4741 gtttttatac cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa 4860

4801 ctcaaaaaga ttcttaatta agtactcgcc tatgtataaa cttacataaa tctttttatt 4860

ORF 1 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa 4920

4861 tgtttatccc caaggcgcgt gtaaaggggc ttttcacggt ggactttaac atttgcaatt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 4980

4921 ataaaacaat tttaagcgca atttaaaaac aatttagtcg agtaaaaaat tggttatccg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 5040

4981 gctttagccg ttttagggaa tatttagttt tcttatctgg ctctatccca actcacaaca 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 5100

5041 aggtcaaacc ttgttctcag gtgataattt cttgcacctg aggttgcagt ttcccgcttt 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 5160

5101 ttggcagata gtcccgctac cgggtgatgc acttggtagt gggattagtt caaaaaaccc 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 5220

5161 cagctccacg gcatttcgtg atttagcctt gggatttccc tcgggggcta aatctcgaac 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 5280

5221 tgcccctttc ggccgcttgc accgctcttt ccttcccttc tttcgctttc ctcgcccgcg 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 5340

5281 atcccgcgac cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc ggcgcgaatt 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgcgccgcta cagggcgcgt cccattcgcc a......... .......... .......... 5371

5341 acgcggcgat gtcccgcgca gggtaagcgg t......... .......... .......... 5371

.......... .......... .......... .......... .......... ..........