Sequence of pET-29_b

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Sequence of pET-29_b_(+)

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Sequence of pET-29 b (+) : 5370 BP; 1271 A; 1387 C; 1443 G; 1269 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206),NcoI(211) ........

181 cgacggagct cgaattcgga tccgatatcg ccatggaacc gcgtggcacc agggtaccca 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccttgg cgcaccgtgg tcccatgggt 240

.......... .......... .......... .......... .......... ..........

.......... .......... BstBI(267) .......... .......... NdeI(294)

241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcatatgta 300

241 ctagacccga caggtacacg accgcaagct taaatcgtcg tcgccaaaga aagtatacat 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(332) .......... ..........

301 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa 360

301 atagaggaag aatttcaatt tgttttaata aagatctccc cttaacaata ggcgagtgtt 360

....... T7_transl_en_RBS ORF 2 ....... lacO ..........

.......... .......... .......... .......... .......... ..........

361 ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tcgatctcga tcctctacgc 420

361 aaggggatat cactcagcat aattaaagcg ccctagctct agctagagct aggagatgcg 420

.......... T7_pro ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 480

421 gcctgcgtag caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg 480

.......... ...... ORF 2 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

481 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 540

481 gctgtagtgg ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc 540

pBRrevBam_pri .. ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc 600

541 gcacccatac caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaacgtacg 600

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 660

601 tggtaaggaa cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta 660

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct 720

661 cgtcctcagc gtattccctc tcgcagctct agggcctgtg gtagcttacc gcgttttgga 720

......... tet(564-300) ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac 780

721 aagcgccata ccgtactatc gcgggccttc tctcagttaa gtcccaccac ttacactttg 780

.......... .......... ORF 2 .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 840

781 gtcattgcaa tatgctacag cgtctcatac ggccacagag aatagtctgg caaagggcgc 840

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 900

841 accacttggt ccggtcggtg caaagacgct tttgcgccct ttttcacctt cgccgctacc 900

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>ggcgggcaaa
901 cggagctgaa ttacattccc aaccgcgtgg cacaacaact cagtcgttgc 960

901 gcctcgactt aatgtaaggg ttggcgcacc gtgttgttga ccgcccgttt gtcagcaacg 960

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>gtcgcaaatt
961 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcggcga 1020

961 actaaccgca acggtggagg tcagaccggg acgtgcgcgg cagcgtttaa cagcgccgct 1020

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 1080

1021 aatttagagc gcggctagtt gacccacggt cgcaccacca cagctaccat cttgcttcgc 1080

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1138)

1081 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 1140

1081 cgcagcttcg gacatttcgc cgccacgtgt tagaagagcg cgttgcgcag tcacccgact 1140

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 1200

1141 agtaattgat aggcgaccta ctggtcctac ggtaacgaca ccttcgacgg acgtgattac 1200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 1260

1201 aaggccgcaa taaagaacta cagagactgg tctgtgggta gttgtcataa taaaagaggg 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 1320

1261 tacttctgcc atgcgctgac ccgcacctcg tagaccagcg taacccagtg gtcgtttagc 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... ApaI(1335) .......... .......... .......... ..........

1321 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 1380

1321 gcgacaatcg cccgggtaat tcaagacaga gccgcgcaga cgcagaccga ccgaccgtat 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 1440

1381 ttatagagtg agcgttagtt taagtcggct atcgccttgc ccttccgctg acctcacggt 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 1500

1441 acaggccaaa agttgtttgg tacgtttacg acttactccc gtagcaaggg tgacgctacg 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 1560

1501 accaacggtt gctagtctac cgcgacccgc gttacgcgcg gtaatggctc aggcccgacg 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgttggtgc ggacatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 1620

1561 cgcaaccacg cctgtagagc catcacccta tgctgctatg gcttctgtcg agtacaatat 1620

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(1630) .......... .......... .......... .......... ..........

1621 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 1680

1621 agggcggcaa ttggtggtag tttgtcctaa aagcggacga ccccgtttgg tcgcacctgg 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 1740

1681 cgaacgacgt tgagagagtc ccggtccgcc acttcccgtt agtcgacaac gggcagagtg 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 1800

1741 accacttttc tttttggtgg gaccgcgggt tatgcgtttg gcggagaggg gcgcgcaacc 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 1860

1801 ggctaagtaa ttacgtcgac cgtgctgtcc aaagggctga cctttcgccc gtcactcgcg 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg 1920

1861 ttgcgttaat tacattcaat cgagtgagta atccgtggcc ctagagctgg ctacgggaac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 1980

1921 tctcggaagt tgggtcagtc gaggaaggcc acccgcgccc cgtactgata gcagcggcgt 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc 2040

1981 gaatactgac agaagaaata gtacgttgag catcctgtcc acggccgtcg cgagacccag 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta 2100

2041 taaaagccgc tcctggcgaa agcgacctcg cgctgctact agccggacag cgaacgccat 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc 2160

2101 aagccttaga acgtgcggga gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2206) ..........

2161 ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc 2220

2161 ccgctcttcg tccggtaata gcggccgtac cgccggggtg cccacgcgta ctagcacgag 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca 2280

2221 gacagcaact cctgggccga tccgaccgcc ccaacggaat gaccaatcgt cttacttagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca 2340

2281 ggctatgcgc tcgcttgcac ttcgctgacg acgacgtttt gcagacgctg gactcgttgt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc 2400

2341 tgtacttacc agaagccaaa ggcacaaagc atttcagacc tttgcgcctt cagtcgcggg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct 2460

2401 acgtggtaat acaaggccta gacgtagcgt cctacgacga ccgatgggac accttgtgga 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc 2520

2461 tgtagacata attgcttcgc gaccgtaact gggactcact aaaaagagac cagggcggcg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca 2580

2521 taggtatggc ggtcaacaaa tgggagtgtt gcaaggtcat tggcccgtac aagtagtagt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga 2640

2581 cattgggcat agcactcgta ggagagagca aagtagccat agtaatgggg gtacttgtct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc 2700

2641 ttagggggaa tgtgcctccg tagtcactgg tttgtccttt tttggcggga attgtaccgg 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat 2760

2701 gcgaaatagt cttcggtctg taattgcgaa gacctctttg agttgctcga cctgcgccta 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc 2820

2761 cttgtccgtc tgtagacact tagcgaagtg ctggtgcgac tactcgaaat ggcgtcgacg 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 2880

2821 gagcgcgcaa agccactact gccacttttg gagactgtgt acgtcgaggg cctctgccag 2880

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2881 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 2940

2881 tgtcgaacag acattcgcct acggccctcg tctgttcggg cagtcccgcg cagtcgccca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact 3000

2941 caaccgccca cagccccgcg tcggtactgg gtcagtgcat cgctatcgcc tcacatatga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga 3060

3001 ccgaattgat acgccgtagt ctcgtctaac atgactctca cgtggtatat acgccacact 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 3120

3061 ttatggcgtg tctacgcatt cctcttttat ggcgtagtcc gcgagaaggc gaaggagcga 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3180

3121 gtgactgagc gacgcgagcc agcaagccga cgccgctcgc catagtcgag tgagtttccg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3240

3181 ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3300

3241 ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3360

3301 ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3420

3361 tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3480

3421 ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3540

3481 atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3600

3541 cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3660

3601 gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3720

3661 tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3780

3721 atcttcctgt cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 3840

3781 accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 3900

3841 cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac 3960

3901 cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact tgttattttg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 4020

3961 acagacgaat gtatttgtca ttatgttccc cacaatactc ggtataagtt gccctttgca 4020

.......... .......... .......... ......... ORF 3 KanR2 .........

.......... .......... .......... .......... .......... ..........

4021 cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg 4080

4021 gaacgagatc cggcgctaat ttaaggttgt acctacgact aaatataccc atatttaccc 4080

.......... ......... ORF 3 KanR2 ......... .......... ..........

NruI(4084) .......... .......... .......... .......... ..........

4081 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg 4140

4081 gagcgctatt acagcccgtt agtccacgct gttagatagc taacataccc ttcgggctac 4140

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga 4200

4141 gcggtctcaa caaagacttt gtaccgtttc catcgcaacg gttactacaa tgtctactct 4200

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc 4260

4201 accagtctga tttgaccgac tgccttaaat acggagaagg ctggtagttc gtaaaatagg 4260

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... SmaI(4301) ..........

4261 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg 4320

4261 catgaggact actacgtacc aatgagtggt gacgctaggg gcccttttgt cgtaaggtcc 4320

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc 4380

4321 ataatcttct tataggacta agtccacttt tataacaact acgcgaccgt cacaaggacg 4380

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc 4440

4381 cggccaacgt aagctaagga caaacattaa caggaaaatt gtcgctagcg cataaagcag 4440

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg 4500

4441 agcgagtccg cgttagtgct tacttattgc caaaccaact acgctcacta aaactactgc 4500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct 4560

4501 tcgcattacc gaccggacaa cttgttcaga cctttcttta cgtatttgaa aacggtaaga 4560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg 4620

4561 gtggcctaag tcagcagtga gtaccactaa agagtgaact attggaataa aaactgctcc 4620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc 4680

4621 cctttaatta tccaacataa ctacaacctg ctcagcctta gcgtctggct atggtcctag 4680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc 4740

4681 aacggtagga taccttgacg gagccactca aaagaggaag taatgtcttt gccgaaaaag 4740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg 4800

4741 tttttatacc ataactatta ggactatact tatttaacgt caaagtaaac tacgagctac 4800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa 4860

4801 tcaaaaagat tcttaattaa gtactcgcct atgtataaac ttacataaat ctttttattt 4860

ORF 3 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat 4920

4861 gtttatcccc aaggcgcgtg taaaggggct tttcacggtg gactttaaca tttgcaatta 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 4980

4921 taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 5040

4981 ctttagccgt tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 5100

5041 ggtcaaacct tgttctcagg tgataatttc ttgcacctga ggttgcagtt tcccgctttt 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 5160

5101 tggcagatag tcccgctacc gggtgatgca cttggtagtg ggattagttc aaaaaacccc 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 5220

5161 agctccacgg catttcgtga tttagccttg ggatttccct cgggggctaa atctcgaact 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 5280

5221 gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc tcgcccgcga 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 5340

5281 tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgccgctac agggcgcgtc ccattcgcca .......... .......... .......... 5370

5341 cgcggcgatg tcccgcgcag ggtaagcggt .......... .......... .......... 5370

.......... .......... .......... .......... .......... ..........