Sequence of pET-29_c

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Sequence of pET-29_c_(+)

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Sequence of pET-29 c (+) : 5372 BP; 1273 A; 1388 C; 1442 G; 1269 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(209),NcoI(213) ........

181 cgacggagct cgaattcgga tccacagata tcccatggaa ccgcgtggca ccagggtacc 240

181 gctgcctcga gcttaagcct aggtgtctat agggtacctt ggcgcaccgt ggtcccatgg 240

.......... .......... .......... .......... .......... ..........

BglII(242) .......... BstBI(269) .......... .......... NdeI(296)

241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatatg 300

241 gtctagaccc gacaggtaca cgaccgcaag cttaaatcgt cgtcgccaaa gaaagtatac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(334) .......... ..........

301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 360

301 atatagagga agaatttcaa tttgttttaa taaagatctc cccttaacaa taggcgagtg 360

....... T7_transl_en_RBS ORF 1 ....... lacO ..........

.......... .......... .......... .......... .......... ..........

361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac 420

361 ttaaggggat atcactcagc ataattaaag cgccctagct ctagctagag ctaggagatg 420

.......... T7_pro ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 480

421 cggcctgcgt agcaccggcc gtagtggccg cggtgtccac gccaacgacc gcggatatag 480

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 540

481 cggctgtagt ggctacccct tctagcccga gcggtgaagc ccgagtactc gcgaacaaag 540

pBRrevBam_pri .. ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat 600

541 ccgcacccat accaccgtcc ggggcaccgg ccccctgaca acccgcggta gaggaacgta 600

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 660

601 cgtggtaagg aacgccgccg ccacgagttg ccggagttgg atgatgaccc gacgaaggat 660

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac 720

661 tacgtcctca gcgtattccc tctcgcagct ctagggcctg tggtagctta ccgcgttttg 720

......... tet(564-300) ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa 780

721 gaaagcgcca taccgtacta tcgcgggcct tctctcagtt aagtcccacc acttacactt 780

.......... .......... ORF 1 .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 840

781 tggtcattgc aatatgctac agcgtctcat acggccacag agaatagtct ggcaaagggc 840

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 900

841 gcaccacttg gtccggtcgg tgcaaagacg cttttgcgcc ctttttcacc ttcgccgcta 900

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>ctggcgggca
901 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa aacagtcgtt 960

901 ccgcctcgac ttaatgtaag ggttggcgca ccgtgttgtt gaccgcccgt ttgtcagcaa 960

.......... ....... ORF 1 lacI ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>ccgtcgcaaa
961 gctgattggc gttgccacct ccagtctggc cctgcacgcg ttgtcgcggc 1020

961 cgactaaccg caacggtgga ggtcagaccg ggacgtgcgc ggcagcgttt aacagcgccg 1020

.......... ....... ORF 1 lacI ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 1080

1021 ctaatttaga gcgcggctag ttgacccacg gtcgcaccac cacagctacc atcttgcttc 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1140)

1081 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 1140

1081 gccgcagctt cggacatttc gccgccacgt gttagaagag cgcgttgcgc agtcacccga 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 1200

1141 ctagtaattg ataggcgacc tactggtcct acggtaacga caccttcgac ggacgtgatt 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 1260

1201 acaaggccgc aataaagaac tacagagact ggtctgtggg tagttgtcat aataaaagag 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 1320

1261 ggtacttctg ccatgcgctg acccgcacct cgtagaccag cgtaacccag tggtcgttta 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1337) .......... .......... .......... ..........

1321 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 1380

1321 gcgcgacaat cgcccgggta attcaagaca gagccgcgca gacgcagacc gaccgaccgt 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 1440

1381 atttatagag tgagcgttag tttaagtcgg ctatcgcctt gcccttccgc tgacctcacg 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 1500

1441 gtacaggcca aaagttgttt ggtacgttta cgacttactc ccgtagcaag ggtgacgcta 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 1560

1501 cgaccaacgg ttgctagtct accgcgaccc gcgttacgcg cggtaatggc tcaggcccga 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgcgttggt gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta 1620

1561 cgcgcaacca cgcctgtaga gccatcaccc tatgctgcta tggcttctgt cgagtacaat 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... HpaI(1632) .......... .......... .......... ..........

1621 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 1680

1621 atagggcggc aattggtggt agtttgtcct aaaagcggac gaccccgttt ggtcgcacct 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 1740

1681 ggcgaacgac gttgagagag tcccggtccg ccacttcccg ttagtcgaca acgggcagag 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 1800

1741 tgaccacttt tctttttggt gggaccgcgg gttatgcgtt tggcggagag gggcgcgcaa 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 1860

1801 ccggctaagt aattacgtcg accgtgctgt ccaaagggct gacctttcgc ccgtcactcg 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct 1920

1861 cgttgcgtta attacattca atcgagtgag taatccgtgg ccctagagct ggctacggga 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg 1980

1921 actctcggaa gttgggtcag tcgaggaagg ccacccgcgc cccgtactga tagcagcggc 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg 2040

1981 gtgaatactg acagaagaaa tagtacgttg agcatcctgt ccacggccgt cgcgagaccc 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg 2100

2041 agtaaaagcc gctcctggcg aaagcgacct cgcgctgcta ctagccggac agcgaacgcc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt 2160

2101 ataagcctta gaacgtgcgg gagcgagttc ggaagcagtg accagggcgg tggtttgcaa 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2208) ..........

2161 tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc 2220

2161 agccgctctt cgtccggtaa tagcggccgt accgccgggg tgcccacgcg tactagcacg 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 2280

2221 aggacagcaa ctcctgggcc gatccgaccg ccccaacgga atgaccaatc gtcttactta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 2340

2281 gtggctatgc gctcgcttgc acttcgctga cgacgacgtt ttgcagacgc tggactcgtt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 2400

2341 gttgtactta ccagaagcca aaggcacaaa gcatttcaga cctttgcgcc ttcagtcgcg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac 2460

2401 ggacgtggta atacaaggcc tagacgtagc gtcctacgac gaccgatggg acaccttgtg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc 2520

2461 gatgtagaca taattgcttc gcgaccgtaa ctgggactca ctaaaaagag accagggcgg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat 2580

2521 cgtaggtatg gcggtcaaca aatgggagtg ttgcaaggtc attggcccgt acaagtagta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca 2640

2581 gtcattgggc atagcactcg taggagagag caaagtagcc atagtaatgg gggtacttgt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg 2700

2641 ctttaggggg aatgtgcctc cgtagtcact ggtttgtcct tttttggcgg gaattgtacc 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 2760

2701 gggcgaaata gtcttcggtc tgtaattgcg aagacctctt tgagttgctc gacctgcgcc 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 2820

2761 tacttgtccg tctgtagaca cttagcgaag tgctggtgcg actactcgaa atggcgtcga 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 2880

2821 cggagcgcgc aaagccacta ctgccacttt tggagactgt gtacgtcgag ggcctctgcc 2880

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2881 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 2940

2881 agtgtcgaac agacattcgc ctacggccct cgtctgttcg ggcagtcccg cgcagtcgcc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 3000

2941 cacaaccgcc cacagccccg cgtcggtact gggtcagtgc atcgctatcg cctcacatat 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt 3060

3001 gaccgaattg atacgccgta gtctcgtcta acatgactct cacgtggtat atacgccaca 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 3120

3061 ctttatggcg tgtctacgca ttcctctttt atggcgtagt ccgcgagaag gcgaaggagc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 3180

3121 gagtgactga gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 3240

3181 cgccattatg ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 3300

3241 ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 3360

3301 gcggggggac tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 3420

3361 cctgatattt ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 3480

3421 tgggacggcg aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 3540

3481 gtatcgagtg cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 3600

3541 cacgtgcttg gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 3660

3601 aggttgggcc attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 3720

3661 tctcgctcca tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 3780

3721 tgatcttcct gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 3840

3781 caaccatcga gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 3900

3841 ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa 3960

3901 cccagactgc gagtcacctt gcttttgagt gcaattccct aaaaccagta cttgttattt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 4020

3961 tgacagacga atgtatttgt cattatgttc cccacaatac tcggtataag ttgccctttg 4020

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... ..........

4021 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg 4080

4021 cagaacgaga tccggcgcta atttaaggtt gtacctacga ctaaatatac ccatatttac 4080

.......... ......... ORF 2 KanR2 ......... .......... ..........

NruI(4086) .......... .......... .......... .......... ..........

4081 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga 4140

4081 ccgagcgcta ttacagcccg ttagtccacg ctgttagata gctaacatac ccttcgggct 4140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga 4200

4141 acgcggtctc aacaaagact ttgtaccgtt tccatcgcaa cggttactac aatgtctact 4200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat 4260

4201 ctaccagtct gatttgaccg actgccttaa atacggagaa ggctggtagt tcgtaaaata 4260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... SmaI(4303) ..........

4261 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca 4320

4261 ggcatgagga ctactacgta ccaatgagtg gtgacgctag gggccctttt gtcgtaaggt 4320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct 4380

4321 ccataatctt cttataggac taagtccact tttataacaa ctacgcgacc gtcacaagga 4380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg 4440

4381 cgcggccaac gtaagctaag gacaaacatt aacaggaaaa ttgtcgctag cgcataaagc 4440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga 4500

4441 agagcgagtc cgcgttagtg cttacttatt gccaaaccaa ctacgctcac taaaactact 4500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt 4560

4501 gctcgcatta ccgaccggac aacttgttca gacctttctt tacgtatttg aaaacggtaa 4560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga 4620

4561 gagtggccta agtcagcagt gagtaccact aaagagtgaa ctattggaat aaaaactgct 4620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga 4680

4621 cccctttaat tatccaacat aactacaacc tgctcagcct tagcgtctgg ctatggtcct 4680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt 4740

4681 agaacggtag gataccttga cggagccact caaaagagga agtaatgtct ttgccgaaaa 4740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga 4800

4741 agtttttata ccataactat taggactata cttatttaac gtcaaagtaa actacgagct 4800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata 4860

4801 actcaaaaag attcttaatt aagtactcgc ctatgtataa acttacataa atctttttat 4860

ORF 2 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta 4920

4861 ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg tggactttaa catttgcaat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 4980

4921 tataaaacaa ttttaagcgc aatttaaaaa caatttagtc gagtaaaaaa ttggttatcc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 5040

4981 ggctttagcc gttttaggga atatttagtt ttcttatctg gctctatccc aactcacaac 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 5100

5041 aaggtcaaac cttgttctca ggtgataatt tcttgcacct gaggttgcag tttcccgctt 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 5160

5101 tttggcagat agtcccgcta ccgggtgatg cacttggtag tgggattagt tcaaaaaacc 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 5220

5161 ccagctccac ggcatttcgt gatttagcct tgggatttcc ctcgggggct aaatctcgaa 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 5280

5221 ctgccccttt cggccgcttg caccgctctt tccttccctt ctttcgcttt cctcgcccgc 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 5340

5281 gatcccgcga ccgttcacat cgccagtgcg acgcgcattg gtggtgtggg cggcgcgaat 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atgcgccgct acagggcgcg tcccattcgc ca........ .......... .......... 5372

5341 tacgcggcga tgtcccgcgc agggtaagcg gt........ .......... .......... 5372

.......... .......... .......... .......... .......... ..........