Sequence of pET-30_Ek

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Sequence of pET-30_Ek_LIC

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Sequence of pET-30 Ek/LIC : 5439 BP; 1289 A; 1401 C; 1464 G; 1285 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... ....... T7_term T7_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg < font color="#000000" style="font-size:10px" face="Courier">aaagcccgaa 120

.... T7_Terminal_pri T7_term T7_term .... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term T7_term .... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgatatcg ccatggttga ggagaagccc gggctcttgt 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccaact cctcttcggg cccgagaaca 240

.......... .......... .......... .......... .......... ..........

..... KpnI(255),BglII(258) ..... .......... BstBI(285) ..........

241 cgtcgtcatc ggtacccaga tctgggctgt ccatgtgctg gcgttcgaat ttagcagcag 300

241 gcagcagtag ccatgggtct agacccgaca ggtacacgac cgcaagctta aatcgtcgtc 300

EK .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cggtttcttt cataccagaa ccgcgtggca ccagaccaga agaatgatga tgatgatggt 360

301 gccaaagaaa gtatggtctt ggcgcaccgt ggtctggtct tcttactact actactacca 360

.......... .......... .......... .......... .......... ORF 2

NdeI(363) .......... .......... .......... XbaI(401) ..........

361 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc 420

361 cgtatacata tagaggaaga atttcaattt gttttaataa agatctcccc ttaacaatag 420

.......... .... ORF 2 T7_transl_en_RBS .... .......... lacO

.......... .......... .......... .......... .......... ..........

421 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat cgatctcgat 480

421 gcgagtgtta aggggatatc actcagcata attaaagcgc cctagctcta gctagagcta 480

lacO ......... ORF 2 T7_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc 540

481 ggagatgcgg cctgcgtagc accggccgta gtggccgcgg tgtccacgcc aacgaccgcg 540

.......... ......... ORF 2 tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

541 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc 600

541 gatatagcgg ctgtagtggc taccccttct agcccgagcg gtgaagcccg agtactcgcg 600

pBRrevBam_pri .. ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc 660

601 aacaaagccg cacccatacc accgtccggg gcaccggccc cctgacaacc cgcggtagag 660

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg 720

661 gaacgtacgt ggtaaggaac gccgccgcca cgagttgccg gagttggatg atgacccgac 720

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc 780

721 gaaggattac gtcctcagcg tattccctct cgcagctcta gggcctgtgg tagcttaccg 780

......... tet(564-300) ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga 840

781 cgttttggaa agcgccatac cgtactatcg cgggccttct ctcagttaag tcccaccact 840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg 900

841 tacactttgg tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc 900

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag 960

901 aaagggcgca ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc 960

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>acaacaactg
961 cggcgatggc ggagctgaat tacattccca accgcgtggc gcgggcaaac 1020

961 gccgctaccg cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg 1020

.......... .......... ORF 2 lacI ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg 1080

1021 tcagcaacga ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac 1080

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag 1140

1081 agcgccgcta atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc 1140

ORF 2 .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca 1200

1141 ttgcttcgcc gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt 1200

.......... .......... lacI ORF 3 .......... .......... ..........

BclI(1207) .......... .......... .......... .......... ..........

1201 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct 1260

1201 cacccgacta gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta 1320

1261 cgtgattaca aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc 1380

1321 aaaagagggt acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... ApaI(1404) .......... .......... ..........

1381 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg 1440

1381 tcgtttagcg cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact 1500

1441 cgaccgtatt tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca 1560

1501 cctcacggta caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt 1620

1561 gacgctacga ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccgggctgcg cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct 1680

1621 ggcccgacgc gcaaccacgc ctgtagagcc atcaccctat gctgctatgg cttctgtcga 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... HpaI(1699) .......... .......... .......... ..........

1681 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca 1740

1681 gtacaatata gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc 1800

1741 cgcacctggc gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc 1860

1801 ggcagagtga ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 1920

1861 cgcgcaaccg gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg 1980

1921 tcactcgcgt tgcgttaatt acattcaatc gagtgagtaa tccgtggccc tagagctggc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 2040

1981 tacgggaact ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag 2040

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

2041 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 2100

2041 cagcggcgtg aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 2160

2101 gagacccagt aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc 2220

2161 gaacgccata agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... FspI(2275)

2221 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg 2280

2221 tttgcaaagc cgctcttcgt ccggtaatag cggccgtacc gccggggtgc ccacgcgtac 2280

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 2340

2281 tagcacgagg acagcaactc ctgggccgat ccgaccgccc caacggaatg accaatcgtc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 2400

2341 ttacttagtg gctatgcgct cgcttgcact tcgctgacga cgacgttttg cagacgctgg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 2460

2401 actcgttgtt gtacttacca gaagccaaag gcacaaagca tttcagacct ttgcgccttc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt 2520

2461 agtcgcggga cgtggtaata caaggcctag acgtagcgtc ctacgacgac cgatgggaca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg 2580

2521 ccttgtggat gtagacataa ttgcttcgcg accgtaactg ggactcacta aaaagagacc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt 2640

2581 agggcggcgt aggtatggcg gtcaacaaat gggagtgttg caaggtcatt ggcccgtaca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc 2700

2641 agtagtagtc attgggcata gcactcgtag gagagagcaa agtagccata gtaatggggg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt 2760

2701 tacttgtctt tagggggaat gtgcctccgt agtcactggt ttgtcctttt ttggcgggaa 2760

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2761 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg 2820

2761 ttgtaccggg cgaaatagtc ttcggtctgt aattgcgaag acctctttga gttgctcgac 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac 2880

2821 ctgcgcctac ttgtccgtct gtagacactt agcgaagtgc tggtgcgact actcgaaatg 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 2940

2881 gcgtcgacgg agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc 2940

.......... .......... ROP .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 3000

2941 ctctgccagt gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 3060

3001 agtcgcccac aaccgcccac agccccgcgt cggtactggg tcagtgcatc gctatcgcct 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 3120

3061 cacatatgac cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtggtatata 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 3180

3121 cgccacactt tatggcgtgt ctacgcattc ctcttttatg gcgtagtccg cgagaaggcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3240

3181 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3300

3241 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3360

3301 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3420

3361 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3480

3421 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3540

3481 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3600

3541 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3660

3601 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3720

3661 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3780

3721 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3840

3781 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3900

3841 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3960

3901 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa 4020

3961 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac 4080

4021 gttattttga cagacgaatg tatttgtcat tatgttcccc acaatactcg gtataagttg 4080

.......... .......... .......... .......... ......... ORF 3 KanR2

.......... .......... .......... .......... .......... ..........

4081 gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 4140

4081 ccctttgcag aacgagatcc ggcgctaatt taaggttgta cctacgacta aatataccca 4140

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... NruI(4153) .......... .......... .......... ..........

4141 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga 4200

4141 tatttacccg agcgctatta cagcccgtta gtccacgctg ttagatagct aacataccct 4200

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 4260

4201 tcgggctacg cggtctcaac aaagactttg taccgtttcc atcgcaacgg ttactacaat 4260

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 4320

4261 gtctactcta ccagtctgat ttgaccgact gccttaaata cggagaaggc tggtagttcg 4320

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag 4380

4321 taaaataggc atgaggacta ctacgtacca atgagtggtg acgctagggg cccttttgtc 4380

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 4440

4381 gtaaggtcca taatcttctt ataggactaa gtccactttt ataacaacta cgcgaccgtc 4440

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 4500

4441 acaaggacgc ggccaacgta agctaaggac aaacattaac aggaaaattg tcgctagcgc 4500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 4560

4501 ataaagcaga gcgagtccgc gttagtgctt acttattgcc aaaccaacta cgctcactaa 4560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 4620

4561 aactactgct cgcattaccg accggacaac ttgttcagac ctttctttac gtatttgaaa 4620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 4680

4621 acggtaagag tggcctaagt cagcagtgag taccactaaa gagtgaacta ttggaataaa 4680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 4740

4681 aactgctccc ctttaattat ccaacataac tacaacctgc tcagccttag cgtctggcta 4740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 4800

4741 tggtcctaga acggtaggat accttgacgg agccactcaa aagaggaagt aatgtctttg 4800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 4860

4801 ccgaaaaagt ttttatacca taactattag gactatactt atttaacgtc aaagtaaact 4860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag 4920

4861 acgagctact caaaaagatt cttaattaag tactcgccta tgtataaact tacataaatc 4920

ORF 3 KanR2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta 4980

4921 tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actttaacat 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 5040

4981 ttgcaattat aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 5100

5041 gttatccggc tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 5160

5101 tcacaacaag gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 5220

5161 cccgcttttt ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 5280

5221 aaaaacccca gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 5340

5281 tctcgaactg cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 5400

5341 cgcccgcgat cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca. .......... .......... 5439

5401 cgcgaattac gcggcgatgt cccgcgcagg gtaagcggt. .......... .......... 5439

.......... .......... .......... .......... .......... ..........