'LOCUS pET-30 Xa/LIC 5448 bp DNA SYN 18-Sep-2007 DEFINITION pET-30 Xa/LIC ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5448 /organism="pET-30 Xa/LIC" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(382..398) /label="T7_transl_en_RBS" misc_feature complement(416..443) /label="lacO" promoter complement(443..461) /label="T7_promoter" misc_feature complement(549..568) /label="pBRrevBam_primer" gene complement(497..760) /label="tet(564-300)" /gene="tet(564-300)" CDS 353..1102 /label="ORF frame 2" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" misc_feature 843..1934 /label="lacI" CDS 975..1934 /label="ORF frame 3" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(1993..2268) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2743..2934 /label="ROP" misc_feature complement(2928..2950) /label="pGEX_3_primer" rep_origin complement(3349..3968) /label="pBR322_origin" CDS 4074..4889 /label="KanR2" /gene="KanR2" /note="ORF frame 3" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" gene 4074..4889 /label="KanR2" /gene="KanR2" rep_origin complement(5114..5420) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat 361 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga 421 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc 481 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca 781 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca 841 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 901 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 961 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 1021 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 1081 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 1141 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 1201 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 1261 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 1321 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 1381 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 1441 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 1501 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 1561 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 1621 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg 1681 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 1741 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 1801 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 1921 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga 1981 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg 2281 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 2341 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 2401 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 2461 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 2521 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 2581 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 2641 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 2701 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 2761 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 2821 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 2881 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 3121 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4021 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 4081 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt 4141 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat 4201 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 4261 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 4321 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 4381 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 4441 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 4501 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 4561 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 4621 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 4681 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 4741 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 4801 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 4861 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa 4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 4981 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 5041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 5101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 5161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 5221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 5281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 5341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 5401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca //pET-30 Xa/LIC "Times New RomanCT7_term"Times New RomanC f1_origin"Times New RomanCEWT7_Terminal_pri"Times New RomanCɆ6xHis"Times New RomanCaNORF 2"Times New Roman~T7_transl_en_RBS"Times New RomanClacO"Times New RomanCT7_pro"Times New Roman* tet(564-300)"Times New RomanCɆ%8 pBRrevBam_pri"Times New Roman*CKlacI"Times New RomanCɆORF 3"Times New Roman* tet(852-576)"Times New RomanC v ROP"Times New RomanCp  pGEX_3_pri"Times New RomanCɆ  pBR322_origin"Times New RomanORF 3"Times New RomanCKanR2"Times New Roman ͝XhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),EcoRV(206),NcoI(211),MscI(216),AgeI(257),KpnI(264),BglII(267),BstBI(294)"Times New Roman fKF NdeI(372),XbaI(410)"Times New Romant:8 BclI"Times New Roman ApaI"Times New Roman HpaI"Times New Romany #* FspI"Times New Roman9 NruI"Times New RomanBo SmaI"Times New Romanplh{ao test"Times New RomanO,SN test"Times New Roman11#(--