Sequence of pET-30_Xa

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Sequence of pET-30_Xa_LIC

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Sequence of pET-30 Xa/LIC : 5448 BP; 1297 A; 1407 C; 1464 G; 1280 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccggtc tcctctcaat ctcgctggga 240

.......... .......... .......... .......... .......... ..........

..... AgeI(257) KpnI(264),BglII(267) ..... .......... BstBI(294)

241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt 300

241 gttatggcct cggtggtggc catgggtcta gacccgacag gtacacgacc gcaagcttaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat 360

301 atcgtcgtcg ccaaagaaag tatggtcttg gcgcaccgtg gtctggtctt cttactacta 360

.......... .......... .......... .......... .......... ..........

.......... NdeI(372) .......... .......... XbaI(410) ..........

361 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga 420

361 ctactaccac gtatacatat agaggaagaa tttcaatttg ttttaataaa gatctcccct 420

.......... .... ORF 2 T7_transl_en_RBS .... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc 480

421 taacaatagg cgagtgttaa ggggatatca ctcagcataa ttaaagcgcc ctagctctag 480

.......... lacO ORF 2 T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

481 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 540

481 ctagagctag gagatgcggc ctgcgtagca ccggccgtag tggccgcggt gtccacgcca 540

.......... ......... ORF 2 tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 600

541 acgaccgcgg atatagcggc tgtagtggct accccttcta gcccgagcgg tgaagcccga 600

.... pBRrevBam_pri ORF 2 tet(564-300) .... .......... ..........

.......... .......... .......... .......... .......... ..........

601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 660

601 gtactcgcga acaaagccgc acccatacca ccgtccgggg caccggcccc ctgacaaccc 660

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 720

661 gcggtagagg aacgtacgtg gtaaggaacg ccgccgccac gagttgccgg agttggatga 720

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca 780

721 tgacccgacg aaggattacg tcctcagcgt attccctctc gcagctctag ggcctgtggt 780

......... tet(564-300) ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca 840

781 agcttaccgc gttttggaaa gcgccatacc gtactatcgc gggccttctc tcagttaagt 840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 900

841 cccaccactt acactttggt cattgcaata tgctacagcg tctcatacgg ccacagagaa 900

.......... .......... ORF 2 lacI .......... ..........

.......... .......... .......... .......... .......... ..........

901 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 960

901 tagtctggca aagggcgcac cacttggtcc ggtcggtgca aagacgcttt tgcgcccttt 960

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 1020

961 ttcaccttcg ccgctaccgc ctcgacttaa tgtaagggtt ggcgcaccgt gttgttgacc 1020

.......... .......... ORF 2 lacI ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 1080

1021 gcccgtttgt cagcaacgac taaccgcaac ggtggaggtc agaccgggac gtgcgcggca 1080

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 1140

1081 gcgtttaaca gcgccgctaa tttagagcgc ggctagttga cccacggtcg caccaccaca 1140

.......... ORF 2 lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 1200

1141 gctaccatct tgcttcgccg cagcttcgga catttcgccg ccacgtgtta gaagagcgcg 1200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... BclI(1216) .......... .......... .......... ..........

1201 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 1260

1201 ttgcgcagtc acccgactag taattgatag gcgacctact ggtcctacgg taacgacacc 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 1320

1261 ttcgacggac gtgattacaa ggccgcaata aagaactaca gagactggtc tgtgggtagt 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 1380

1321 tgtcataata aaagagggta cttctgccat gcgctgaccc gcacctcgta gaccagcgta 1380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... ApaI(1413) .......... ..........

1381 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 1440

1381 acccagtggt cgtttagcgc gacaatcgcc cgggtaattc aagacagagc cgcgcagacg 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 1500

1441 cagaccgacc gaccgtattt atagagtgag cgttagttta agtcggctat cgccttgccc 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 1560

1501 ttccgctgac ctcacggtac aggccaaaag ttgtttggta cgtttacgac ttactcccgt 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 1620

1561 agcaagggtg acgctacgac caacggttgc tagtctaccg cgacccgcgt tacgcgcggt 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg 1680

1621 aatggctcag gcccgacgcg caaccacgcc tgtagagcca tcaccctatg ctgctatggc 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... HpaI(1708) .......... .......... ..........

1681 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 1740

1681 ttctgtcgag tacaatatag ggcggcaatt ggtggtagtt tgtcctaaaa gcggacgacc 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 1800

1741 ccgtttggtc gcacctggcg aacgacgttg agagagtccc ggtccgccac ttcccgttag 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 1860

1801 tcgacaacgg gcagagtgac cacttttctt tttggtggga ccgcgggtta tgcgtttggc 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 1920

1861 ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg tgctgtccaa agggctgacc 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga 1980

1921 tttcgcccgt cactcgcgtt gcgttaatta cattcaatcg agtgagtaat ccgtggccct 1980

lacI ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 2040

1981 agagctggct acgggaactc tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg 2040

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 2100

2041 tactgatagc agcggcgtga atactgacag aagaaatagt acgttgagca tcctgtccac 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 2160

2101 ggccgtcgcg agacccagta aaagccgctc ctggcgaaag cgacctcgcg ctgctactag 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 2220

2161 ccggacagcg aacgccataa gccttagaac gtgcgggagc gagttcggaa gcagtgacca 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg 2280

2221 gggcggtggt ttgcaaagcc gctcttcgtc cggtaatagc ggccgtaccg ccggggtgcc 2280

.......... ......... tet(852-576) ......... .......... ..........

FspI(2284) .......... .......... .......... .......... ..........

2281 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 2340

2281 cacgcgtact agcacgagga cagcaactcc tgggccgatc cgaccgcccc aacggaatga 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 2400

2341 ccaatcgtct tacttagtgg ctatgcgctc gcttgcactt cgctgacgac gacgttttgc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 2460

2401 agacgctgga ctcgttgttg tacttaccag aagccaaagg cacaaagcat ttcagacctt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 2520

2461 tgcgccttca gtcgcgggac gtggtaatac aaggcctaga cgtagcgtcc tacgacgacc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 2580

2521 gatgggacac cttgtggatg tagacataat tgcttcgcga ccgtaactgg gactcactaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 2640

2581 aaagagacca gggcggcgta ggtatggcgg tcaacaaatg ggagtgttgc aaggtcattg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 2700

2641 gcccgtacaa gtagtagtca ttgggcatag cactcgtagg agagagcaaa gtagccatag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 2760

2701 taatgggggt acttgtcttt agggggaatg tgcctccgta gtcactggtt tgtccttttt 2760

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

2761 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 2820

2761 tggcgggaat tgtaccgggc gaaatagtct tcggtctgta attgcgaaga cctctttgag 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 2880

2821 ttgctcgacc tgcgcctact tgtccgtctg tagacactta gcgaagtgct ggtgcgacta 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 2940

2881 ctcgaaatgg cgtcgacgga gcgcgcaaag ccactactgc cacttttgga gactgtgtac 2940

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 3000

2941 gtcgagggcc tctgccagtg tcgaacagac attcgcctac ggccctcgtc tgttcgggca 3000

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 3060

3001 gtcccgcgca gtcgcccaca accgcccaca gccccgcgtc ggtactgggt cagtgcatcg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 3120

3061 ctatcgcctc acatatgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 3180

3121 tggtatatac gccacacttt atggcgtgtc tacgcattcc tcttttatgg cgtagtccgc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3240

3181 gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3300

3241 tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3360

3301 cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg 3360

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3420

3361 caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3480

3421 caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3540

3481 cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3600

3541 ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca tccagcaagc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3660

3601 gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3720

3661 attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3780

3721 accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3840

3781 cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg acttcggtca 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3900

3841 atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3960

3901 accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4020

3961 aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 4080

4021 ccagtacttg ttattttgac agacgaatgt atttgtcatt atgttcccca caatactcgg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt 4140

4081 tataagttgc cctttgcaga acgagatccg gcgctaattt aaggttgtac ctacgactaa 4140

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... NruI(4162) .......... .......... ..........

4141 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat 4200

4141 atatacccat atttacccga gcgctattac agcccgttag tccacgctgt tagatagcta 4200

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 4260

4201 acataccctt cgggctacgc ggtctcaaca aagactttgt accgtttcca tcgcaacggt 4260

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 4320

4261 tactacaatg tctactctac cagtctgatt tgaccgactg ccttaaatac ggagaaggct 4320

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... SmaI(4379)

4321 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 4380

4321 ggtagttcgt aaaataggca tgaggactac tacgtaccaa tgagtggtga cgctaggggc 4380

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 4440

4381 ccttttgtcg taaggtccat aatcttctta taggactaag tccactttta taacaactac 4440

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 4500

4441 gcgaccgtca caaggacgcg gccaacgtaa gctaaggaca aacattaaca ggaaaattgt 4500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 4560

4501 cgctagcgca taaagcagag cgagtccgcg ttagtgctta cttattgcca aaccaactac 4560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 4620

4561 gctcactaaa actactgctc gcattaccga ccggacaact tgttcagacc tttctttacg 4620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 4680

4621 tatttgaaaa cggtaagagt ggcctaagtc agcagtgagt accactaaag agtgaactat 4680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 4740

4681 tggaataaaa actgctcccc tttaattatc caacataact acaacctgct cagccttagc 4740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 4800

4741 gtctggctat ggtcctagaa cggtaggata ccttgacgga gccactcaaa agaggaagta 4800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 4860

4801 atgtctttgc cgaaaaagtt tttataccat aactattagg actatactta tttaacgtca 4860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa 4920

4861 aagtaaacta cgagctactc aaaaagattc ttaattaagt actcgcctat gtataaactt 4920

......... ORF 3 KanR2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 4980

4921 acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 5040

4981 ctttaacatt tgcaattata aaacaatttt aagcgcaatt taaaaacaat ttagtcgagt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 5100

5041 aaaaaattgg ttatccggct ttagccgttt tagggaatat ttagttttct tatctggctc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 5160

5101 tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt gcacctgagg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 5220

5161 ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg gtgatgcact tggtagtggg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 5280

5221 attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg atttccctcg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 5340

5281 ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc gctctttcct tcccttcttt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 5400

5341 cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca.. .......... 5448

5401 tgtgggcggc gcgaattacg cggcgatgtc ccgcgcaggg taagcggt.. .......... 5448

.......... .......... .......... .......... .......... ..........