91 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA 201 TCCGATATCA GCCATGGCCT TGTCGTCGTC GTCGGTACCC AGATCTGGGC 251 TGTCCATGTG CTGGCGTTCG AATTTAGCAG CAGCGGTTTC TTTCATACCA 301 GAACCGCGTG GCACCAGACC AGAAGAATGA TGATGATGAT GGTGCATATG 351 TATATCTCCT TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAATTGTT 401 ATCCGCTCAC AATTCCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA 451 GATCGATCTC GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC 501 GCCACAGGTG CGGTTGCTGG CGCCTATATC GCCGACATCA CCGATGGGGA 551 AGATCGGGCT CGCCACTTCG GGCTCATGAG CGCTTGTTTC GGCGTGGGTA 601 TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT CTCCTTGCAT 651 GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG 701 CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GATCCCGGAC 751 ACCATCGAAT GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA 801 AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG TTATACGATG 851 TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC 901 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT 951 GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA 1001 AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG 1051 CCGTCGCAAA TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC 1101 CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA GCCTGTAAAG 1151 CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC 1201 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA 1251 TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA 1301 TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC 1351 GCATTGGGTC ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT 1401 CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC ACTCGCAATC 1451 AAATTCAGCC GATAGCGGAA CGGGAAGGCG ACTGGAGTGC CATGTCCGGT 1501 TTTCAACAAA CCATGCAAAT GCTGAATGAG GGCATCGTTC CCACTGCGAT 1551 GCTGGTTGCC AACGATCAGA TGGCGCTGGG CGCAATGCGC GCCATTACCG 1601 AGTCCGGGCT GCGCGTTGGT GCGGACATCT CGGTAGTGGG ATACGACGAT 1651 ACCGAAGACA GCTCATGTTA TATCCCGCCG TTAACCACCA TCAAACAGGA 1701 TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC 1751 AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA 1801 AGAAAAACCA CCCTGGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 1851 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG 1901 GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC ATTAGGCACC 1951 GGGATCTCGA CCGATGCCCT TGAGAGCCTT CAACCCAGTC AGCTCCTTCC 2001 GGTGGGCGCG GGGCATGACT ATCGTCGCCG CACTTATGAC TGTCTTCTTT 2051 ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTGGG TCATTTTCGG 2101 CGAGGACCGC TTTCGCTGGA GCGCGACGAT GATCGGCCTG TCGCTTGCGG 2151 TATTCGGAAT CTTGCACGCC CTCGCTCAAG CCTTCGTCAC TGGTCCCGCC 2201 ACCAAACGTT TCGGCGAGAA GCAGGCCATT ATCGCCGGCA TGGCGGCCCC 2251 ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC 2301 GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG 2351 TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT 2401 GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC 2451 CCTGCACCAT TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC 2501 TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT GACCCTGAGT 2551 GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC 2601 AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC 2651 ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC 2701 TTACACGGAG GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG 2751 CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA ACTCAACGAG 2801 CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC 2851 TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA 2901 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG 2951 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 3001 GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA 3051 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 3101 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 3151 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 3201 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3251 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3301 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 3351 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 3401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 3451 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 3501 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3551 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 3601 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 3651 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 3701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 3751 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 3801 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 3851 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 3901 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 3951 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 4001 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG 4051 AGCCATATTC AACGGGAAAC GTCTTGCTCT AGGCCGCGAT TAAATTCCAA 4101 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC 4151 AATCAGGTGC GACAATCTAT CGATTGTATG GGAAGCCCGA TGCGCCAGAG 4201 TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 4251 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA 4301 AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC 4351 CCCGGGAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 4401 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC 4451 CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG 4501 GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 4551 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC 4601 TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT 4651 GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 4701 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT 4751 GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT 4801 GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 4851 TGAGTTTTTC TAAGAATTAA TTCATGAGCG GATACATATT TGAATGTATT 4901 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 4951 ACCTGAAATT GTAAACGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT 5001 GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT 5051 TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG 5101 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA 5151 AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA 5201 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG 5251 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA 5301 AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA 5351 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT 5401 ACAGGGCGCG TCCCATTCGC CA pET-30 a (+) "Times New RomanCT7_term"Times New RomanCEWT7_Terminal_pri"Times New RomanCɆ6xHis"Times New RomanEK"Times New RomanCG4ORF 3"Times New RomanCdtT7_transl_en_RBS"Times New RomanClacO"Times New RomanCɆT7_pro"Times New Roman*C tet(564-300)"Times New Roman  pBRrevBam_pri"Times New Roman*CɆ1tlacI"Times New RomantORF 1"Times New Roman*C tet(852-576)"Times New RomanC \ ROP"Times New RomanCɆV l  pGEX_3_pri"Times New Roman f pBR322_origin"Times New RomanCORF 1"Times New RomanCKanR2"Times New RomanCɆ f1_origin"Times New Roman ͉XhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),EcoRV(206),NcoI(212),KpnI(238),BglII(241),BstBI(268)"Times New Roman ^h`* NdeI(346),XbaI(384)"Times New RomanZ'9 BclI"Times New Roman ApaI"Times New Romank HpaI"Times New Romant$}'*#1 FspI"Times New RomanZ NruI"Times New Roman(g SmaI"Times New Roman{wsp~ test"Times New RomanO"6"LN test"Times New Roman22 66