Sequence of pET-30_a

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Sequence of pET-30_a_(+)

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Sequence of pET-30 a (+) : 5422 BP; 1285 A; 1398 C; 1457 G; 1282 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206) NcoI(212) ........

181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc 240

181 gctgcctcga gcttaagcct aggctatagt cggtaccgga acagcagcag cagccatggg 240

.......... .......... .......... .......... EK ..........

BglII(241) .......... BstBI(268) .......... .......... ..........

241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca 300

241 tctagacccg acaggtacac gaccgcaagc ttaaatcgtc gtcgccaaag aaagtatggt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg tatatctcct 360

301 cttggcgcac cgtggtctgg tcttcttact actactacta ccacgtatac atatagagga 360

.......... .......... .......... .......... ORF 3 ..........

.......... .......... XbaI(384) .......... .......... ..........

361 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta 420

361 agaatttcaa tttgttttaa taaagatctc cccttaacaa taggcgagtg ttaaggggat 420

T7_transl_en_RBS .... ORF 3 .......... lacO ..........

.......... .......... .......... .......... .......... ..........

421 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca 480

421 atcactcagc ataattaaag cgccctagct ctagctagag ctaggagatg cggcctgcgt 480

T7_pro .......... ORF 3 .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

481 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 540

481 agcaccggcc gtagtggccg cggtgtccac gccaacgacc gcggatatag cggctgtagt 540

.......... ...... ORF 3 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 600

541 ggctacccct tctagcccga gcggtgaagc ccgagtactc gcgaacaaag ccgcacccat 600

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 660

601 accaccgtcc ggggcaccgg ccccctgaca acccgcggta gaggaacgta cgtggtaagg 660

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 720

661 aacgccgccg ccacgagttg ccggagttgg atgatgaccc gacgaaggat tacgtcctca 720

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt 780

721 gcgtattccc tctcgcagct ctagggcctg tggtagctta ccgcgttttg gaaagcgcca 780

tet(564-300) ........ ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa ac cagtaacg 840

781 taccgtacta tcgcgggcct tctctcagtt aagtcccacc acttacactt tggtcattgc 840

.......... .......... ORF 3 .......... lacI ..........

.......... .......... .......... .......... .......... ..........

841 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 900

841 aatatgctac agcgtctcat acggccacag agaatagtct ggcaaagggc gcaccacttg 900

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 960

901 gtccggtcgg tgcaaagacg cttttgcgcc ctttttcacc ttcgccgcta ccgcctcgac 960

.......... .......... ORF 3 lacI .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........
< td>aacagtcgtt
961 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca gctgattggc 1020

961 ttaatgtaag ggttggcgca ccgtgttgtt gaccgcccgt ttgtcagcaa cgactaaccg 1020

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1080

1021 caacggtgga ggtcagaccg ggacgtgcgc ggcagcgttt aacagcgccg ctaatttaga 1080

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1140

1081 gcgcggctag ttgacccacg gtcgcaccac cacagctacc atcttgcttc gccgcagctt 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... BclI(1190) ..........

1141 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1200

1141 cggacatttc gccgccacgt gttagaagag cgcgttgcgc agtcacccga ctagtaattg 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1260

1201 ataggcgacc tactggtcct acggtaacga caccttcgac ggacgtgatt acaaggccgc 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1320

1261 aataaagaac tacagagact ggtctgtggg tagttgtcat aataaaagag ggtacttctg 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1380

1321 ccatgcgctg acccgcacct cgtagaccag cgtaacccag tggtcgttta gcgcgacaat 1380

.......... .......... lacI ORF 1 .......... .......... ..........

ApaI(1387) .......... .......... .......... .......... ..........

1381 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1440

1381 cgcccgggta attcaagaca gagccgcgca gacgcagacc gaccgaccgt atttatagag 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1500

1441 tgagcgttag tttaagtcgg ctatcgcctt gcccttccgc tgacctcacg gtacaggcca 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1560

1501 aaagttgttt ggtacgttta cgacttactc ccgtagcaag ggtgacgcta cgaccaacgg 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 1620

1561 ttgctagtct accgcgaccc gcgttacgcg cggtaatggc tcaggcccga cgcgcaacca 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 1680

1621 cgcctgtaga gccatcaccc tatgctgcta tggcttctgt cgagtacaat atagggcggc 1680

.......... .......... lacI ORF 1 .......... .......... ..........

HpaI(1682) .......... .......... .......... .......... ..........

1681 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 1740

1681 aattggtggt agtttgtcct aaaagcggac gaccccgttt ggtcgcacct ggcgaacgac 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 1800

1741 gttgagagag tcccggtccg ccacttcccg ttagtcgaca acgggcagag tgaccacttt 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 1860

1801 tctttttggt gggaccgcgg gttatgcgtt tggcggagag gggcgcgcaa ccggctaagt 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 1920

1861 aattacgtcg accgtgctgt ccaaagggct gacctttcgc ccgtcactcg cgttgcgtta 1920

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt 1980

1921 attacattca atcgagtgag taatccgtgg ccctagagct ggctacggga actctcggaa 1980

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

1981 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 2040

1981 gttgggtcag tcgaggaagg ccacccgcgc cccgtactga tagcagcggc gtgaatactg 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg 2100

2041 acagaagaaa tagtacgttg agcatcctgt ccacggccgt cgcgagaccc agtaaaagcc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat 2160

2101 gctcctggcg aaagcgacct cgcgctgcta ctagccggac agcgaacgcc ataagcctta 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa 2220

2161 gaacgtgcgg gagcgagttc ggaagcagtg accagggcgg tggtttgcaa agccgctctt 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... FspI(2258) .......... ..........

2221 gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc tcctgtcgtt 2280

2221 cgtccggtaa tagcggccgt accgccgggg tgcccacgcg tactagcacg aggacagcaa 2280

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 2340

2281 ctcctgggcc gatccgaccg ccccaacgga atgaccaatc gtcttactta gtggctatgc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 2400

2341 gctcgcttgc acttcgctga cgacgacgtt ttgcagacgc tggactcgtt gttgtactta 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat 2460

2401 ccagaagcca aaggcacaaa gcatttcaga cctttgcgcc ttcagtcgcg ggacgtggta 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt 2520

2461 atacaaggcc tagacgtagc gtcctacgac gaccgatggg acaccttgtg gatgtagaca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac 2580

2521 taattgcttc gcgaccgtaa ctgggactca ctaaaaagag accagggcgg cgtaggtatg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg 2640

2581 gcggtcaaca aatgggagtg ttgcaaggtc attggcccgt acaagtagta gtcattgggc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc 2700

2641 atagcactcg taggagagag caaagtagcc atagtaatgg gggtacttgt ctttaggggg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat 2760

2701 aatgtgcctc cgtagtcact ggtttgtcct tttttggcgg gaattgtacc gggcgaaata 2760

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc 2820

2761 gtcttcggtc tgtaattgcg aagacctctt tgagttgctc gacctgcgcc tacttgtccg 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg 2880

2821 tctgtagaca cttagcgaag tgctggtgcg actactcgaa atggcgtcga cggagcgcgc 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 2940

2881 aaagccacta ctgccacttt tggagactgt gtacgtcgag ggcctctgcc agtgtcgaac 2940

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 3000

2941 agacattcgc ctacggccct cgtctgttcg ggcagtcccg cgcagtcgcc cacaaccgcc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 3060

3001 cacagccccg cgtcggtact gggtcagtgc atcgctatcg cctcacatat gaccgaattg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc 3120

3061 atacgccgta gtctcgtcta acatgactct cacgtggtat atacgccaca ctttatggcg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 3180

3121 tgtctacgca ttcctctttt atggcgtagt ccgcgagaag gcgaaggagc gagtgactga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3240

3181 gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3300

3241 ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3360

3301 tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3420

3361 tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3480

3421 ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3540

3481 aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3600

3541 cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3660

3601 gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3720

3661 attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 3780

3721 tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3840

3781 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3900

3841 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3960

3901 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct 4020

3961 gagtcacctt gcttttgagt gcaattccct aaaaccagta cttgttattt tgacagacga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctct 4080

4021 atgtatttgt cattatgttc cccacaatac tcggtataag ttgccctttg cagaacgaga 4080

.......... .......... .......... ......... ORF 1 KanR2 .........

.......... .......... .......... .......... .......... NruI(4136)

4081 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 4140

4081 tccggcgcta atttaaggtt gtacctacga ctaaatatac ccatatttac ccgagcgcta 4140

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag 4200

4141 ttacagcccg ttagtccacg ctgttagata gctaacatac ccttcgggct acgcggtctc 4200

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 4260

4201 aacaaagact ttgtaccgtt tccatcgcaa cggttactac aatgtctact ctaccagtct 4260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 4320

4261 gatttgaccg actgccttaa atacggagaa ggctggtagt tcgtaaaata ggcatgagga 4320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4353) .......... ..........

4321 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa 4380

4321 ctactacgta ccaatgagtg gtgacgctag gggccctttt gtcgtaaggt ccataatctt 4380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 4440

4381 cttataggac taagtccact tttataacaa ctacgcgacc gtcacaagga cgcggccaac 4440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 4500

4441 gtaagctaag gacaaacatt aacaggaaaa ttgtcgctag cgcataaagc agagcgagtc 4500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 4560

4501 cgcgttagtg cttacttatt gccaaaccaa ctacgctcac taaaactact gctcgcatta 4560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 4620

4561 ccgaccggac aacttgttca gacctttctt tacgtatttg aaaacggtaa gagtggccta 4620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 4680

4621 agtcagcagt gagtaccact aaagagtgaa ctattggaat aaaaactgct cccctttaat 4680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 4740

4681 tatccaacat aactacaacc tgctcagcct tagcgtctgg ctatggtcct agaacggtag 4740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 4800

4741 gataccttga cggagccact caaaagagga agtaatgtct ttgccgaaaa agtttttata 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 4860

4801 ccataactat taggactata cttatttaac gtcaaagtaa actacgagct actcaaaaag 4860

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4920

4861 attcttaatt aagtactcgc ctatgtataa acttacataa atctttttat ttgtttatcc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt 4980

4921 ccaaggcgcg tgtaaagggg cttttcacgg tggactttaa catttgcaat tataaaacaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 5040

4981 ttttaagcgc aatttaaaaa caatttagtc gagtaaaaaa ttggttatcc ggctttagcc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 5100

5041 gttttaggga atatttagtt ttcttatctg gctctatccc aactcacaac aaggtcaaac 5100

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 5160

5101 cttgttctca ggtgataatt tcttgcacct gaggttgcag tttcccgctt tttggcagat 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 5220

5161 agtcccgcta ccgggtgatg cacttggtag tgggattagt tcaaaaaacc ccagctccac 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 5280

5221 ggcatttcgt gatttagcct tgggatttcc ctcgggggct aaatctcgaa ctgccccttt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 5340

5281 cggccgcttg caccgctctt tccttccctt ctttcgcttt cctcgcccgc gatcccgcga 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 5400

5341 ccgttcacat cgccagtgcg acgcgcattg gtggtgtggg cggcgcgaat tacgcggcga 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 acagggcgcg tcccattcgc ca........ .......... .......... .......... 5422

5401 tgtcccgcgc agggtaagcg gt........ .......... .......... .......... 5422

.......... .......... .......... .......... .......... ..........