Sequence of pET-30_b

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Sequence of pET-30_b_(+)

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Sequence of pET-30 b (+) : 5421 BP; 1284 A; 1398 C; 1457 G; 1282 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206),NcoI(211) ........

181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccggaa cagcagcagc agccatgggt 240

.......... .......... .......... .......... EK ..........

.......... .......... BstBI(267) .......... .......... ..........

241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag 300

241 ctagacccga caggtacacg accgcaagct taaatcgtcg tcgccaaaga aagtatggtc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt 360

301 ttggcgcacc gtggtctggt cttcttacta ctactactac cacgtataca tatagaggaa 360

.......... .......... .......... .......... ORF 2 ..........

.......... .......... XbaI(383) .......... .......... ..........

361 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat 420

361 gaatttcaat ttgttttaat aaagatctcc ccttaacaat aggcgagtgt taaggggata 420

T7_transl_en_RBS .... ORF 2 .......... lacO ..........

.......... .......... .......... .......... .......... ..........

421 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat 480

421 tcactcagca taattaaagc gccctagctc tagctagagc taggagatgc ggcctgcgta 480

T7_pro .......... ORF 2 .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac 540

481 gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc ggctgtagtg 540

.......... ...... ORF 2 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat 600

541 gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc cgcacccata 600

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct 660

601 ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac gtggtaagga 660

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc 720

661 acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt acgtcctcag 720

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta 780

721 cgtattccct ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg aaagcgccat 780

tet(564-300) ........ ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa cc agtaacgt 840

781 accgtactat cgcgggcctt ctctcagtta agtcccacca cttacacttt ggtcattgca 840

.......... .......... ORF 2 .......... lacI ..........

.......... .......... .......... .......... .......... ..........

841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 900

841 atatgctaca gcgtctcata cggccacaga gaatagtctg gcaaagggcg caccacttgg 900

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 960

901 tccggtcggt gcaaagacgc ttttgcgccc tttttcacct tcgccgctac cgcctcgact 960

.......... .......... ORF 2 lacI .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........
< td>acagtcgttg
961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa ctgattggcg 1020

961 taatgtaagg gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac gactaaccgc 1020

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 1080

1021 aacggtggag gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc taatttagag 1080

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 1140

1081 cgcggctagt tgacccacgg tcgcaccacc acagctacca tcttgcttcg ccgcagcttc 1140

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... BclI(1189) ..........

1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 1200

1141 ggacatttcg ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac tagtaattga 1200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 1260

1201 taggcgacct actggtccta cggtaacgac accttcgacg gacgtgatta caaggccgca 1260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 1320

1261 ataaagaact acagagactg gtctgtgggt agttgtcata ataaaagagg gtacttctgc 1320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 1380

1321 catgcgctga cccgcacctc gtagaccagc gtaacccagt ggtcgtttag cgcgacaatc 1380

.......... .......... lacI ORF 3 .......... .......... ..........

ApaI(1386) .......... .......... .......... .......... ..........

1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 1440

1381 gcccgggtaa ttcaagacag agccgcgcag acgcagaccg accgaccgta tttatagagt 1440

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 1500

1441 gagcgttagt ttaagtcggc tatcgccttg cccttccgct gacctcacgg tacaggccaa 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 1560

1501 aagttgtttg gtacgtttac gacttactcc cgtagcaagg gtgacgctac gaccaacggt 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 1620

1561 tgctagtcta ccgcgacccg cgttacgcgc ggtaatggct caggcccgac gcgcaaccac 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 1680

1621 gcctgtagag ccatcaccct atgctgctat ggcttctgtc gagtacaata tagggcggca 1680

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(1681) .......... .......... .......... .......... ..........

1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 1740

1681 attggtggta gtttgtccta aaagcggacg accccgtttg gtcgcacctg gcgaacgacg 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 1800

1741 ttgagagagt cccggtccgc cacttcccgt tagtcgacaa cgggcagagt gaccactttt 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 1860

1801 ctttttggtg ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac cggctaagta 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 1920

1861 attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc gttgcgttaa 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc 1980

1921 ttacattcaa tcgagtgagt aatccgtggc cctagagctg gctacgggaa ctctcggaag 1980

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

1981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 2040

1981 ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg tgaatactga 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 2100

2041 cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca gtaaaagccg 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 2160

2101 ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca taagccttag 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 2220

2161 aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa gccgctcttc 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... FspI(2257) .......... ..........

2221 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg 2280

2221 gtccggtaat agcggccgta ccgccggggt gcccacgcgt actagcacga ggacagcaac 2280

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 2340

2281 tcctgggccg atccgaccgc cccaacggaa tgaccaatcg tcttacttag tggctatgcg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 2400

2341 ctcgcttgca cttcgctgac gacgacgttt tgcagacgct ggactcgttg ttgtacttac 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 2460

2401 cagaagccaa aggcacaaag catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 2520

2461 tacaaggcct agacgtagcg tcctacgacg accgatggga caccttgtgg atgtagacat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 2580

2521 aattgcttcg cgaccgtaac tgggactcac taaaaagaga ccagggcggc gtaggtatgg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 2640

2581 cggtcaacaa atgggagtgt tgcaaggtca ttggcccgta caagtagtag tcattgggca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 2700

2641 tagcactcgt aggagagagc aaagtagcca tagtaatggg ggtacttgtc tttaggggga 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 2760

2701 atgtgcctcc gtagtcactg gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag 2760

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 2820

2761 tcttcggtct gtaattgcga agacctcttt gagttgctcg acctgcgcct acttgtccgt 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 2880

2821 ctgtagacac ttagcgaagt gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 2940

2881 aagccactac tgccactttt ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca 2940

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 3000

2941 gacattcgcc tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 3060

3001 acagccccgc gtcggtactg ggtcagtgca tcgctatcgc ctcacatatg accgaattga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca 3120

3061 tacgccgtag tctcgtctaa catgactctc acgtggtata tacgccacac tttatggcgt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 3180

3121 gtctacgcat tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3240

3181 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3300

3241 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 3360

3301 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 3420

3361 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 3480

3421 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 3540

3481 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 3600

3541 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 3660

3601 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 3720

3661 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 3780

3721 acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 3840

3781 tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 3900

3841 aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 3960

3901 atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt 4020

3961 agtcaccttg cttttgagtg caattcccta aaaccagtac ttgttatttt gacagacgaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta 4080

4021 tgtatttgtc attatgttcc ccacaatact cggtataagt tgccctttgc agaacgagat 4080

.......... .......... .......... ......... ORF 3 KanR2 .........

.......... .......... .......... .......... .......... NruI(4135)

4081 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 4140

4081 ccggcgctaa tttaaggttg tacctacgac taaatatacc catatttacc cgagcgctat 4140

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 4200

4141 tacagcccgt tagtccacgc tgttagatag ctaacatacc cttcgggcta cgcggtctca 4200

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 4260

4201 acaaagactt tgtaccgttt ccatcgcaac ggttactaca atgtctactc taccagtctg 4260

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 4320

4261 atttgaccga ctgccttaaa tacggagaag gctggtagtt cgtaaaatag gcatgaggac 4320

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4352) .......... ..........

4321 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag 4380

4321 tactacgtac caatgagtgg tgacgctagg ggcccttttg tcgtaaggtc cataatcttc 4380

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 4440

4381 ttataggact aagtccactt ttataacaac tacgcgaccg tcacaaggac gcggccaacg 4440

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 4500

4441 taagctaagg acaaacatta acaggaaaat tgtcgctagc gcataaagca gagcgagtcc 4500

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 4560

4501 gcgttagtgc ttacttattg ccaaaccaac tacgctcact aaaactactg ctcgcattac 4560

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 4620

4561 cgaccggaca acttgttcag acctttcttt acgtatttga aaacggtaag agtggcctaa 4620

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 4680

4621 gtcagcagtg agtaccacta aagagtgaac tattggaata aaaactgctc ccctttaatt 4680

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 4740

4681 atccaacata actacaacct gctcagcctt agcgtctggc tatggtccta gaacggtagg 4740

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 4800

4741 ataccttgac ggagccactc aaaagaggaa gtaatgtctt tgccgaaaaa gtttttatac 4800

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 4860

4801 cataactatt aggactatac ttatttaacg tcaaagtaaa ctacgagcta ctcaaaaaga 4860

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 4920

4861 ttcttaatta agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta 4980

4921 caaggcgcgt gtaaaggggc ttttcacggt ggactttaac atttgcaatt ataaaacaat 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 5040

4981 tttaagcgca atttaaaaac aatttagtcg agtaaaaaat tggttatccg gctttagccg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 5100

5041 ttttagggaa tatttagttt tcttatctgg ctctatccca actcacaaca aggtcaaacc 5100

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 5160

5101 ttgttctcag gtgataattt cttgcacctg aggttgcagt ttcccgcttt ttggcagata 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 5220

5161 gtcccgctac cgggtgatgc acttggtagt gggattagtt caaaaaaccc cagctccacg 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 5280

5221 gcatttcgtg atttagcctt gggatttccc tcgggggcta aatctcgaac tgcccctttc 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 5340

5281 ggccgcttgc accgctcttt ccttcccttc tttcgctttc ctcgcccgcg atcccgcgac 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 5400

5341 cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc ggcgcgaatt acgcggcgat 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cagggcgcgt cccattcgcc a......... .......... .......... .......... 5421

5401 gtcccgcgca gggtaagcgg t......... .......... .......... .......... 5421

.......... .......... .......... .......... .......... ..........