Sequence of pET-30_c

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Sequence of pET-30_c_(+)

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Sequence of pET-30 c (+) : 5423 BP; 1286 A; 1399 C; 1456 G; 1282 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(209),NcoI(213) ........

181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc 240

181 gctgcctcga gcttaagcct aggtgtctat agggtaccgg aacagcagca gcagccatgg 240

.......... .......... .......... .......... EK ..........

BglII(242) .......... BstBI(269) .......... .......... ..........

241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc 300

241 gtctagaccc gacaggtaca cgaccgcaag cttaaatcgt cgtcgccaaa gaaagtatgg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat gtatatctcc 360

301 tcttggcgca ccgtggtctg gtcttcttac tactactact accacgtata catatagagg 360

.......... .......... .......... .......... ORF 1 ..........

.......... .......... XbaI(385) .......... .......... ..........

361 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct 420

361 aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt gttaagggga 420

T7_transl_en_RBS .... ORF 1 .......... lacO ..........

.......... .......... .......... .......... .......... ..........

421 atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta cgccggacgc 480

421 tatcactcag cataattaaa gcgccctagc tctagctaga gctaggagat gcggcctgcg 480

T7_pro .......... ORF 1 .......... ......... tet(564-300)

.......... .......... .......... .......... .......... ..........

481 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc 540

481 tagcaccggc cgtagtggcc gcggtgtcca cgccaacgac cgcggatata gcggctgtag 540

.......... ...... ORF 1 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt 600

541 tggctacccc ttctagcccg agcggtgaag cccgagtact cgcgaacaaa gccgcaccca 600

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc 660

601 taccaccgtc cggggcaccg gccccctgac aacccgcggt agaggaacgt acgtggtaag 660

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag 720

661 gaacgccgcc gccacgagtt gccggagttg gatgatgacc cgacgaagga ttacgtcctc 720

.......... ...... ORF 1 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg 780

721 agcgtattcc ctctcgcagc tctagggcct gtggtagctt accgcgtttt ggaaagcgcc 780

tet(564-300) ........ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aa ccagtaac 840

781 ataccgtact atcgcgggcc ttctctcagt taagtcccac cacttacact ttggtcattg 840

.......... .......... ORF 1 .......... lacI ..........

.......... .......... .......... .......... .......... ..........

841 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 900

841 caatatgcta cagcgtctca tacggccaca gagaatagtc tggcaaaggg cgcaccactt 900

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 960

901 ggtccggtcg gtgcaaagac gcttttgcgc cctttttcac cttcgccgct accgcctcga 960

.......... .......... ORF 1 lacI .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........
< td>aaacagtcgt
961 gaattacatt cccaaccgcg tggcacaaca actggcgggc tgctgattgg 1020

961 cttaatgtaa gggttggcgc accgtgttgt tgaccgcccg tttgtcagca acgactaacc 1020

.......... ....... ORF 1 lacI ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 1080

1021 gcaacggtgg aggtcagacc gggacgtgcg cggcagcgtt taacagcgcc gctaatttag 1080

.......... ....... ORF 1 lacI ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 1140

1081 agcgcggcta gttgacccac ggtcgcacca ccacagctac catcttgctt cgccgcagct 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1191)

1141 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 1200

1141 tcggacattt cgccgccacg tgttagaaga gcgcgttgcg cagtcacccg actagtaatt 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 1260

1201 gataggcgac ctactggtcc tacggtaacg acaccttcga cggacgtgat tacaaggccg 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 1320

1261 caataaagaa ctacagagac tggtctgtgg gtagttgtca taataaaaga gggtacttct 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 1380

1321 gccatgcgct gacccgcacc tcgtagacca gcgtaaccca gtggtcgttt agcgcgacaa 1380

.......... .......... lacI ORF 2 .......... .......... ..........

ApaI(1388) .......... .......... .......... .......... ..........

1381 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 1440

1381 tcgcccgggt aattcaagac agagccgcgc agacgcagac cgaccgaccg tatttataga 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 1500

1441 gtgagcgtta gtttaagtcg gctatcgcct tgcccttccg ctgacctcac ggtacaggcc 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 1560

1501 aaaagttgtt tggtacgttt acgacttact cccgtagcaa gggtgacgct acgaccaacg 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 1620

1561 gttgctagtc taccgcgacc cgcgttacgc gcggtaatgg ctcaggcccg acgcgcaacc 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 1680

1621 acgcctgtag agccatcacc ctatgctgct atggcttctg tcgagtacaa tatagggcgg 1680

.......... .......... lacI ORF 2 .......... .......... ..........

HpaI(1683) .......... .......... .......... .......... ..........

1681 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 1740

1681 caattggtgg tagtttgtcc taaaagcgga cgaccccgtt tggtcgcacc tggcgaacga 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 1800

1741 cgttgagaga gtcccggtcc gccacttccc gttagtcgac aacgggcaga gtgaccactt 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 1860

1801 ttctttttgg tgggaccgcg ggttatgcgt ttggcggaga ggggcgcgca accggctaag 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1920

1861 taattacgtc gaccgtgctg tccaaagggc tgacctttcg cccgtcactc gcgttgcgtt 1920

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct 1980

1921 aattacattc aatcgagtga gtaatccgtg gccctagagc tggctacggg aactctcgga 1980

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

1981 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 2040

1981 agttgggtca gtcgaggaag gccacccgcg ccccgtactg atagcagcgg cgtgaatact 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 2100

2041 gacagaagaa atagtacgtt gagcatcctg tccacggccg tcgcgagacc cagtaaaagc 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 2160

2101 cgctcctggc gaaagcgacc tcgcgctgct actagccgga cagcgaacgc cataagcctt 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 2220

2161 agaacgtgcg ggagcgagtt cggaagcagt gaccagggcg gtggtttgca aagccgctct 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... FspI(2259) .......... ..........

2221 agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt 2280

2221 tcgtccggta atagcggccg taccgccggg gtgcccacgc gtactagcac gaggacagca 2280

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 2340

2281 actcctgggc cgatccgacc gccccaacgg aatgaccaat cgtcttactt agtggctatg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 2400

2341 cgctcgcttg cacttcgctg acgacgacgt tttgcagacg ctggactcgt tgttgtactt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca 2460

2401 accagaagcc aaaggcacaa agcatttcag acctttgcgc cttcagtcgc gggacgtggt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg 2520

2461 aatacaaggc ctagacgtag cgtcctacga cgaccgatgg gacaccttgt ggatgtagac 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata 2580

2521 ataattgctt cgcgaccgta actgggactc actaaaaaga gaccagggcg gcgtaggtat 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc 2640

2581 ggcggtcaac aaatgggagt gttgcaaggt cattggcccg tacaagtagt agtcattggg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc 2700

2641 catagcactc gtaggagaga gcaaagtagc catagtaatg ggggtacttg tctttagggg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 2760

2701 gaatgtgcct ccgtagtcac tggtttgtcc ttttttggcg ggaattgtac cgggcgaaat 2760

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 2820

2761 agtcttcggt ctgtaattgc gaagacctct ttgagttgct cgacctgcgc ctacttgtcc 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 2880

2821 gtctgtagac acttagcgaa gtgctggtgc gactactcga aatggcgtcg acggagcgcg 2880

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 2940

2881 caaagccact actgccactt ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa 2940

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 3000

2941 cagacattcg cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 3060

3001 ccacagcccc gcgtcggtac tgggtcagtg catcgctatc gcctcacata tgaccgaatt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg 3120

3061 gatacgccgt agtctcgtct aacatgactc tcacgtggta tatacgccac actttatggc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 3180

3121 gtgtctacgc attcctcttt tatggcgtag tccgcgagaa ggcgaaggag cgagtgactg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3240

3181 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3300

3241 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3360

3301 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3420

3361 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3480

3421 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3540

3481 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3600

3541 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3660

3601 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3720

3661 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3780

3721 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3840

3781 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3900

3841 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3960

3901 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc 4020

3961 cgagtcacct tgcttttgag tgcaattccc taaaaccagt acttgttatt ttgacagacg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 4080

4021 aatgtatttg tcattatgtt ccccacaata ctcggtataa gttgcccttt gcagaacgag 4080

.......... .......... .......... ......... ORF 2 KanR2 .........

.......... .......... .......... .......... .......... NruI(4137)

4081 taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 4140

4081 atccggcgct aatttaaggt tgtacctacg actaaatata cccatattta cccgagcgct 4140

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 4200

4141 attacagccc gttagtccac gctgttagat agctaacata cccttcgggc tacgcggtct 4200

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 4260

4201 caacaaagac tttgtaccgt ttccatcgca acggttacta caatgtctac tctaccagtc 4260

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 4320

4261 tgatttgacc gactgcctta aatacggaga aggctggtag ttcgtaaaat aggcatgagg 4320

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... SmaI(4354) .......... ..........

4321 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 4380

4321 actactacgt accaatgagt ggtgacgcta ggggcccttt tgtcgtaagg tccataatct 4380

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 4440

4381 tcttatagga ctaagtccac ttttataaca actacgcgac cgtcacaagg acgcggccaa 4440

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 4500

4441 cgtaagctaa ggacaaacat taacaggaaa attgtcgcta gcgcataaag cagagcgagt 4500

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 4560

4501 ccgcgttagt gcttacttat tgccaaacca actacgctca ctaaaactac tgctcgcatt 4560

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 4620

4561 accgaccgga caacttgttc agacctttct ttacgtattt gaaaacggta agagtggcct 4620

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 4680

4621 aagtcagcag tgagtaccac taaagagtga actattggaa taaaaactgc tcccctttaa 4680

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 4740

4681 ttatccaaca taactacaac ctgctcagcc ttagcgtctg gctatggtcc tagaacggta 4740

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 4800

4741 ggataccttg acggagccac tcaaaagagg aagtaatgtc tttgccgaaa aagtttttat 4800

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 4860

4801 accataacta ttaggactat acttatttaa cgtcaaagta aactacgagc tactcaaaaa 4860

.......... ......... ORF 2 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4920

4861 gattcttaat taagtactcg cctatgtata aacttacata aatcttttta tttgtttatc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt 4980

4921 cccaaggcgc gtgtaaaggg gcttttcacg gtggacttta acatttgcaa ttataaaaca 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 5040

4981 attttaagcg caatttaaaa acaatttagt cgagtaaaaa attggttatc cggctttagc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 5100

5041 cgttttaggg aatatttagt tttcttatct ggctctatcc caactcacaa caaggtcaaa 5100

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 5160

5101 ccttgttctc aggtgataat ttcttgcacc tgaggttgca gtttcccgct ttttggcaga 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 5220

5161 tagtcccgct accgggtgat gcacttggta gtgggattag ttcaaaaaac cccagctcca 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 5280

5221 cggcatttcg tgatttagcc ttgggatttc cctcgggggc taaatctcga actgcccctt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 5340

5281 tcggccgctt gcaccgctct ttccttccct tctttcgctt tcctcgcccg cgatcccgcg 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 5400

5341 accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa ttacgcggcg 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tacagggcgc gtcccattcg cca....... .......... .......... .......... 5423

5401 atgtcccgcg cagggtaagc ggt....... .......... .......... .......... 5423

.......... .......... .......... .......... .......... ..........