ppS'LOCUS pET-31b(+) 5742 bp DNA SYN 02-Sep-2007 DEFINITION pET-31b(+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5742 /organism="pET-31b(+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(553..569) /label="T7_transl_en_RBS" misc_feature complement(587..614) /label="lacO" promoter complement(614..632) /label="T7_promoter" misc_feature complement(716..735) /label="pBRrevBam_primer" gene complement(664..927) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 1010..2101 /label="lacI" CDS 1142..2101 /label="ORF frame 2" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(2160..2435) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2910..3101 /label="ROP" misc_feature complement(3095..3117) /label="pGEX_3_primer" rep_origin complement(3516..4129) /label="pBR322_origin" CDS complement(4284..5144) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4284..5144) /label="Ampicillin" /gene="Ampicillin" promoter complement(5186..5214) /label="AmpR_promoter" rep_origin complement(5408..5714) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcagcatc tggcatgcgt 181 gaatattctt ctcgccaaac aaggcgcgga tgctcaccac cttgccggcg ccattgaagc 241 gaaagtgatc gatgggcgca actacggtct tgcggccctg atactcgaag ctgacggtga 301 aagcgaaggc cgcttcgttg gcgaccgcgc gtacctcctg cgtcagctcc accgccaaag 361 gcagtttgag cgagttggcg taaaactcac gaatcgcagc cgtaccggac ctgggctcgg 421 aacccacggg gtcttccacc gtggcgtcat cggcaaacag cgcgacgatg ccgtccagat 481 cgccggcatt gagcgcagcc acaaagcgct gtaccacggc ggtgatgtgt tctggggtat 541 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc 601 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc 661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat 721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt 781 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg 841 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc 901 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa 961 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt 1021 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc 1081 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc 1141 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc 1201 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc 1261 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg 1321 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg 1381 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac 1441 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt 1501 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca 1561 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg 1621 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag 1681 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc 1741 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg 1801 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg 1861 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 1921 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 1981 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 2041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 2101 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc 2161 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 2221 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 2281 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg 2341 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac 2401 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg 2461 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 2521 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 2581 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 2641 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 2701 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 2761 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 2821 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 2881 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca 2941 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 3001 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 3061 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 3121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 3181 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 3241 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg 3301 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag ctggtaacag gattagcaga 3901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3961 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 4021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 4081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 4141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 4201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 4261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4321 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4381 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4441 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4501 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4561 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 4621 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4681 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4741 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4801 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4861 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4921 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4981 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 5041 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5101 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5161 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5221 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 5281 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 5341 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 5401 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 5461 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 5521 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 5581 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 5641 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 5701 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca //D pET-31b(+) "Times New RomanpCDT7_term"Times New RomanpCD  f1_origin"Times New RomanpCDEWT7_Terminal_pri"Times New RomanpCɆD6xHis"Times New RomanpD)9T7_transl_en_RBS"Times New RomanpCDKflacO"Times New RomanpCDfxT7_pro"Times New RomanpD tet(564-300)"Times New RomanpCɆD pBRrevBam_pri"Times New RomanpCD5lacI"Times New RomanpCDv5ORF 2"Times New RomanpCɆDp  tet(852-576)"Times New RomanpD^  ROP"Times New RomanpCD -  pGEX_3_pri"Times New RomanpCD ! pBR322_origin"Times New RomanpCɆDORF 3"Times New RomanpD Ampicillin"Times New RomanpCDB^AmpR_pro"Times New Roman pXhoI(158),ClaI(249)"Times New RomanD pNdeI(543),XbaI(581),BglII(647)"Times New RomanD Z pBclI"Times New RomangDoy pApaI"Times New Roman, Dmy pEcoRV(1819),HpaI(1875)"Times New RomanD\enn pPstI"Times New RomanDSOKcDR ptest"Times New RomanOD)0N ptest"Times New RomanPD)n)S' ptest"Times New RomanD:AHH