Sequence of pET-31b(+)

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Sequence of pET-31b(+)

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Sequence of pET-31b(+) : 5742 BP; 1352 A; 1534 C; 1535 G; 1321 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcagcatc tggcatgcgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcgtcgtag accgtacgca 180

T7_term .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaatattctt ctcgccaaac aaggcgcgga tgctcaccac cttgccggcg ccattgaagc 240

181 cttataagaa gagcggtttg ttccgcgcct acgagtggtg gaacggccgc ggtaacttcg 240

.......... .......... .......... .......... .......... ..........

ClaI(249) .......... .......... .......... .......... ..........

241 gaaagtgatc gatgggcgca actacggtct tgcggccctg atactcgaag ctgacggtga 300

241 ctttcactag ctacccgcgt tgatgccaga acgccgggac tatgagcttc gactgccact 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aagcgaaggc cgcttcgttg gcgaccgcgc gtacctcctg cgtcagctcc accgccaaag 360

301 ttcgcttccg gcgaagcaac cgctggcgcg catggaggac gcagtcgagg tggcggtttc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcagtttgag cgagttggcg taaaactcac gaatcgcagc cgtaccggac ctgggctcgg 420

361 cgtcaaactc gctcaaccgc attttgagtg cttagcgtcg gcatggcctg gacccgagcc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacccacggg gtcttccacc gtggcgtcat cggcaaacag cgcgacgatg ccgtccagat 480

421 ttgggtgccc cagaaggtgg caccgcagta gccgtttgtc gcgctgctac ggcaggtcta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgccggcatt gagcgcagcc acaaagcgct gtaccacggc ggtgatgtgt tctggggtat 540

481 gcggccgtaa ctcgcgtcgg tgtttcgcga catggtgccg ccactacaca agaccccata 540

.......... .......... .......... .......... .......... ..........

NdeI(543) .......... .......... .......... .......... ..........

541 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc 600

541 cgtatacata tagaggaaga atttcaattt gttttaataa agatctcccc ttaacaatag 600

.......... ....... T7_transl_en_RBS ....... .......... lacO

.......... .......... .......... .......... BglII(647) ..........

601 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc 660

601 gcgagtgtta aggggatatc actcagcata attaaagcgc cctagctcta gagctaggag 660

lacO .......... T7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat 720

661 atgcggcctg cgtagcaccg gccgtagtgg ccgcggtgtc cacgccaacg accgcggata 720

.......... .......... ......... tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt 780

721 tagcggctgt agtggctacc ccttctagcc cgagcggtga agcccgagta ctcgcgaaca 780

pBRrevBam_pri ..... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg 840

781 aagccgcacc cataccaccg tccggggcac cggccccctg acaacccgcg gtagaggaac 840

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc 900

841 gtacgtggta aggaacgccg ccgccacgag ttgccggagt tggatgatga cccgacgaag 900

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa 960

901 gattacgtcc tcagcgtatt ccctctcgca gctctagggc ctgtggtagc ttaccgcgtt 960

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt 1020

961 ttggaaagcg ccataccgta ctatcgcggg ccttctctca gttaagtccc accacttaca 1020

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

1021 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc 1080

1021 ctttggtcat tgcaatatgc tacagcgtct catacggcca cagagaatag tctggcaaag 1080

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc 1140

1081 ggcgcaccac ttggtccggt cggtgcaaag acgcttttgc gccctttttc accttcgccg 1140

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc 1200

1141 ctaccgcctc gacttaatgt aagggttggc gcaccgtgtt gttgaccgcc cgtttgtcag 1200

.......... .......... lacI ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc 1260

1201 caacgactaa ccgcaacggt ggaggtcaga ccgggacgtg cgcggcagcg tttaacagcg 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg 1320

1261 ccgctaattt agagcgcggc tagttgaccc acggtcgcac caccacagct accatcttgc 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg 1380

1321 ttcgccgcag cttcggacat ttcgccgcca cgtgttagaa gagcgcgttg cgcagtcacc 1380

.......... .......... lacI ORF 2 .......... .......... ..........

BclI(1383) .......... .......... .......... .......... ..........

1381 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac 1440

1381 cgactagtaa ttgataggcg acctactggt cctacggtaa cgacaccttc gacggacgtg 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt 1500

1441 attacaaggc cgcaataaag aactacagag actggtctgt gggtagttgt cataataaaa 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca 1560

1501 gagggtactt ctgccatgcg ctgacccgca cctcgtagac cagcgtaacc cagtggtcgt 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1580) .......... .......... .......... ..........

1561 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg 1620

1561 ttagcgcgac aatcgcccgg gtaattcaag acagagccgc gcagacgcag accgaccgac 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag 1680

1621 cgtatttata gagtgagcgt tagtttaagt cggctatcgc cttgcccttc cgctgacctc 1680

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc 1740

1681 acggtacagg ccaaaagttg tttggtacgt ttacgactta ctcccgtagc aagggtgacg 1740

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg 1800

1741 ctacgaccaa cggttgctag tctaccgcga cccgcgttac gcgcggtaat ggctcaggcc 1800

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg 1860

1801 cgacgcgcaa ccacgcctat agagccatca ccctatgctg ctatggcttc tgtcgagtac 1860

.......... .......... lacI ORF 2 .......... .......... ..........

.......... HpaI(1875) .......... .......... .......... ..........

1861 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 1920

1861 aatatagggc ggcaattggt ggtagtttgt cctaaaagcg gacgaccccg tttggtcgca 1920

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 1980

1921 cctggcgaac gacgttgaga gagtcccggt ccgccacttc ccgttagtcg acaacgggca 1980

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 2040

1981 gagtgaccac ttttcttttt ggtgggaccg cgggttatgc gtttggcgga gaggggcgcg 2040

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 2100

2041 caaccggcta agtaattacg tcgaccgtgc tgtccaaagg gctgaccttt cgcccgtcac 2100

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc 2160

2101 tcgcgttgcg ttaattacat tcaatcgagt gagtaatccg tggccctaga gctggctacg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 2220

2161 ggaactctcg gaagttgggt cagtcgagga aggccacccg cgccccgtac tgatagcagc 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 2280

2221 ggcgtgaata ctgacagaag aaatagtacg ttgagcatcc tgtccacggc cgtcgcgaga 2280

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg 2340

2281 cccagtaaaa gccgctcctg gcgaaagcga cctcgcgctg ctactagccg gacagcgaac 2340

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac 2400

2341 gccataagcc ttagaacgtg cgggagcgag ttcggaagca gtgaccaggg cggtggtttg 2400

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg 2460

2401 caaagccgct cttcgtccgg taatagcggc cgtaccgccg gggtgcccac gcgtactagc 2460

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 2520

2461 acgaggacag caactcctgg gccgatccga ccgccccaac ggaatgacca atcgtcttac 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 2580

2521 ttagtggcta tgcgctcgct tgcacttcgc tgacgacgac gttttgcaga cgctggactc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 2640

2581 gttgttgtac ttaccagaag ccaaaggcac aaagcatttc agacctttgc gccttcagtc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 2700

2641 gcgggacgtg gtaatacaag gcctagacgt agcgtcctac gacgaccgat gggacacctt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 2760

2701 gtggatgtag acataattgc ttcgcgaccg taactgggac tcactaaaaa gagaccaggg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 2820

2761 cggcgtaggt atggcggtca acaaatggga gtgttgcaag gtcattggcc cgtacaagta 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 2880

2821 gtagtcattg ggcatagcac tcgtaggaga gagcaaagta gccatagtaa tgggggtact 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca 2940

2881 tgtctttagg gggaatgtgc ctccgtagtc actggtttgt ccttttttgg cgggaattgt 2940

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2941 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 3000

2941 accgggcgaa atagtcttcg gtctgtaatt gcgaagacct ctttgagttg ctcgacctgc 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 3060

3001 gcctacttgt ccgtctgtag acacttagcg aagtgctggt gcgactactc gaaatggcgt 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 3120

3061 cgacggagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct 3120

.......... .......... ROP .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

3121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 3180

3121 gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 3240

3181 gcccacaacc gcccacagcc ccgcgtcggt actgggtcag tgcatcgcta tcgcctcaca 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg 3300

3241 tatgaccgaa ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg tatatacgcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 3360

3301 acactttatg gcgtgtctac gcattcctct tttatggcgt agtccgcgag aaggcgaagg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 3420

3361 agcgagtgac tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 3480

3421 ttccgccatt atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 3540

3481 tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc 3540

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 3600

3541 gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 3660

3601 tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3720

3661 ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3780

3721 agagtatcga gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3840

3781 acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag ctggtaacag gattagcaga 3900

3841 ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gaccattgtc ctaatcgtct 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3960

3901 cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 4020

3961 tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 4080

4021 ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 4140

4081 gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 4200

4141 agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc taatagtttt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 4260

4201 tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta gatttcatat 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 4320

4261 atactcattt gaaccagact gtcaatggtt acgaattagt cactccgtgg atagagtcgc 4320

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4321 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 4380

4321 tagacagata aagcaagtag gtatcaacgg actgaggggc agcacatcta ttgatgctat 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 4440

4381 gccctcccga atggtagacc ggggtcacga cgttactatg gcgctctggg tgcgagtggc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 4500

4441 cgaggtctaa atagtcgtta tttggtcggt cggccttccc ggctcgcgtc ttcaccagga 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 4560

4501 cgttgaaata ggcggaggta ggtcagataa ttaacaacgg cccttcgatc tcattcatca 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4602) ..........

4561 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 4620

4561 agcggtcaat tatcaaacgc gttgcaacaa cggtaacgac gtccgtagca ccacagtgcg 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 4680

4621 agcagcaaac cataccgaag taagtcgagg ccaagggttg ctagttccgc tcaatgtact 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 4740

4681 agggggtaca acacgttttt tcgccaatcg aggaagccag gaggctagca acagtcttca 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 4800

4741 ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg acgtattaag agaatgacag 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 4860

4801 tacggtaggc attctacgaa aagacactga ccactcatga gttggttcag taagactctt 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 4920

4861 atcacatacg ccgctggctc aacgagaacg ggccgcagtt atgccctatt atggcgcggt 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 4980

4921 gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 5040

4981 tcctagaatg gcgacaactc taggtcaagc tacattgggt gagcacgtgg gttgactaga 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 5100

5041 agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 5160

5101 cgttttttcc cttattcccg ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 5220

5161 ataataactt cgtaaatagt cccaataaca gagtactcgc ctatgtataa acttacataa 5220

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 5280

5221 atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg cttttcacgg tggactttaa 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 5340

5281 catttgcaat tataaaacaa ttttaagcgc aatttaaaaa caatttagtc gagtaaaaaa 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 5400

5341 ttggttatcc ggctttagcc gttttaggga atatttagtt ttcttatctg gctctatccc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 5460

5401 aactcacaac aaggtcaaac cttgttctca ggtgataatt tcttgcacct gaggttgcag 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 5520

5461 tttcccgctt tttggcagat agtcccgcta ccgggtgatg cacttggtag tgggattagt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 5580

5521 tcaaaaaacc ccagctccac ggcatttcgt gatttagcct tgggatttcc ctcgggggct 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 5640

5581 aaatctcgaa ctgccccttt cggccgcttg caccgctctt tccttccctt ctttcgcttt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 5700

5641 cctcgcccgc gatcccgcga ccgttcacat cgccagtgcg acgcgcattg gtggtgtggg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca........ .......... 5742

5701 cggcgcgaat tacgcggcga tgtcccgcgc agggtaagcg gt........ .......... 5742

.......... .......... .......... .......... .......... ..........