Sequence of pET-32_Ek

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Sequence of pET-32_Ek_LIC

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Sequence of pET-32 Ek/LIC : 5917 BP; 1395 A; 1567 C; 1567 G; 1388 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206),NcoI(211) ........

181 cgacggagct cgaattcgga tccgatatcg ccatggttga ggagaagccc gggctcttgt 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccaact cctcttcggg cccgagaaca 240

.......... .......... .......... .......... .......... ..........

..... KpnI(255),BglII(258) ..... .......... BstBI(285) ..........

241 cgtcgtcatc ggtacccaga tctgggctgt ccatgtgctg gcgttcgaat ttagcagcag 300

241 gcagcagtag ccatgggtct agacccgaca ggtacacgac cgcaagctta aatcgtcgtc 300

EK .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cggtttcttt cataccagaa ccgcgtggca ccagaccaga agaatgatga tgatgatggt 360

301 gccaaagaaa gtatggtctt ggcgcaccgt ggtctggtct tcttactact actactacca 360

.......... .......... .......... .......... .......... ORF 2

MscI(368) .......... .......... .......... .......... ..........

361 gcatatggcc agaaccagaa ccggccaggt tagcgtcgag gaactctttc aactgacctt 420

361 cgtataccgg tcttggtctt ggccggtcca atcgcagctc cttgagaaag ttgactggaa 420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tagacagtgc acccactttg gttgccgcca cttcaccgtt tttgaacagc agcagagtcg 480

421 atctgtcacg tgggtgaaac caacggcggt gaagtggcaa aaacttgtcg tcgtctcagc 480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg 540

481 cctatggtgc ctacggtata aagccgcgtc acggtcccaa aactagctac aagtcaaaac 540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg gcgatcattt 600

541 gttgccagtc aaacgggact ataagcagtc gctaaagtag gtcttagccc cgctagtaaa 600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc gctttgagta 660

601 acgtgcctgg cgtggtgaga cgggtcttta gctgctccta gcggggcagg cgaaactcat 660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata tgtatatctc 720

661 gtaggcacag ttttgacagc agtcagtcca cttattaaaa tagcgagtat acatatagag 720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... XbaI(746) .......... .......... ..........

721 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 780

721 gaagaatttc aatttgtttt aataaagatc tccccttaac aataggcgag tgttaagggg 780

T7_transl_en_RBS .... ORF 2 .......... lacO ..........

.......... .......... .......... .......... .......... ..........

781 tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctct acgccggacg 840

781 atatcactca gcataattaa agcgccctag ctctagctag agctaggaga tgcggcctgc 840

T7_pro .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat 900

841 gtagcaccgg ccgtagtggc cgcggtgtcc acgccaacga ccgcggatat agcggctgta 900

.......... ...... ORF 2 tet(564-300) ...... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

901 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg 960

901 gtggctaccc cttctagccc gagcggtgaa gcccgagtac tcgcgaacaa agccgcaccc 960

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt 1020

961 ataccaccgt ccggggcacc ggccccctga caacccgcgg tagaggaacg tacgtggtaa 1020

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga 1080

1021 ggaacgccgc cgccacgagt tgccggagtt ggatgatgac ccgacgaagg attacgtcct 1080

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtcgcataag ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg 1140

1081 cagcgtattc cctctcgcag ctctagggcc tgtggtagct taccgcgttt tggaaagcgc 1140

tet(564-300) ........ ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg a aaccagtaa 1200

1141 cataccgtac tatcgcgggc cttctctcag ttaagtccca ccacttacac tttggtcatt 1200

.......... .......... ORF 2 .......... lacI ..........

.......... .......... .......... .......... .......... ..........

1201 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 1260

1201 gcaatatgct acagcgtctc atacggccac agagaatagt ctggcaaagg gcgcaccact 1260

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 1320

1261 tggtccggtc ggtgcaaaga cgcttttgcg ccctttttca ccttcgccgc taccgcctcg 1320

.......... .......... ORF 2 lacI .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1321 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 1380

1321 acttaatgta agggttggcg caccgtgttg ttgaccgccc gtttgtcagc aacgactaac 1380

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 1440

1381 cgcaacggtg gaggtcagac cgggacgtgc gcggcagcgt ttaacagcgc cgctaattta 1440

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 1500

1441 gagcgcggct agttgaccca cggtcgcacc accacagcta ccatcttgct tcgccgcagc 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1552)

1501 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 1560

1501 ttcggacatt tcgccgccac gtgttagaag agcgcgttgc gcagtcaccc gactagtaat 1560

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 1620

1561 tgataggcga cctactggtc ctacggtaac gacaccttcg acggacgtga ttacaaggcc 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 1680

1621 gcaataaaga actacagaga ctggtctgtg ggtagttgtc ataataaaag agggtacttc 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 1740

1681 tgccatgcgc tgacccgcac ctcgtagacc agcgtaaccc agtggtcgtt tagcgcgaca 1740

.......... .......... lacI ORF 3 .......... .......... ..........

ApaI(1749) .......... .......... .......... .......... ..........

1741 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 1800

1741 atcgcccggg taattcaaga cagagccgcg cagacgcaga ccgaccgacc gtatttatag 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 1860

1801 agtgagcgtt agtttaagtc ggctatcgcc ttgcccttcc gctgacctca cggtacaggc 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 1920

1861 caaaagttgt ttggtacgtt tacgacttac tcccgtagca agggtgacgc tacgaccaac 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 1980

1921 ggttgctagt ctaccgcgac ccgcgttacg cgcggtaatg gctcaggccc gacgcgcaac 1980

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtgcggacat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 2040

1981 cacgcctgta gagccatcac cctatgctgc tatggcttct gtcgagtaca atatagggcg 2040

.......... .......... lacI ORF 3 .......... .......... ..........

HpaI(2044) .......... .......... .......... .......... ..........

2041 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 2100

2041 gcaattggtg gtagtttgtc ctaaaagcgg acgaccccgt ttggtcgcac ctggcgaacg 2100

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 2160

2101 acgttgagag agtcccggtc cgccacttcc cgttagtcga caacgggcag agtgaccact 2160

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 2220

2161 tttctttttg gtgggaccgc gggttatgcg tttggcggag aggggcgcgc aaccggctaa 2220

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 2280

2221 gtaattacgt cgaccgtgct gtccaaaggg ctgacctttc gcccgtcact cgcgttgcgt 2280

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 attaatgtaa gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc 2340

2281 taattacatt caatcgagtg agtaatccgt ggccctagag ctggctacgg gaactctcgg 2340

.......... .......... .......... .......... ......... tet(852-576)

.......... .......... .......... .......... .......... ..........

2341 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg 2400

2341 aagttgggtc agtcgaggaa ggccacccgc gccccgtact gatagcagcg gcgtgaatac 2400

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc 2460

2401 tgacagaaga aatagtacgt tgagcatcct gtccacggcc gtcgcgagac ccagtaaaag 2460

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga 2520

2461 ccgctcctgg cgaaagcgac ctcgcgctgc tactagccgg acagcgaacg ccataagcct 2520

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag 2580

2521 tagaacgtgc gggagcgagt tcggaagcag tgaccagggc ggtggtttgc aaagccgctc 2580

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagcaggcca ttatcgccgg catggcggcc ccacgggtgc gcatgatcgt gctcctgtcg 2640

2581 ttcgtccggt aatagcggcc gtaccgccgg ggtgcccacg cgtactagca cgaggacagc 2640

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata 2700

2641 aactcctggg ccgatccgac cgccccaacg gaatgaccaa tcgtcttact tagtggctat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga 2760

2701 gcgctcgctt gcacttcgct gacgacgacg ttttgcagac gctggactcg ttgttgtact 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc 2820

2761 taccagaagc caaaggcaca aagcatttca gacctttgcg ccttcagtcg cgggacgtgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct 2880

2821 taatacaagg cctagacgta gcgtcctacg acgaccgatg ggacaccttg tggatgtaga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat 2940

2881 cataattgct tcgcgaccgt aactgggact cactaaaaag agaccagggc ggcgtaggta 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc 3000

2941 tggcggtcaa caaatgggag tgttgcaagg tcattggccc gtacaagtag tagtcattgg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc 3060

3001 gcatagcact cgtaggagag agcaaagtag ccatagtaat gggggtactt gtctttaggg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt 3120

3061 ggaatgtgcc tccgtagtca ctggtttgtc cttttttggc gggaattgta ccgggcgaaa 3120

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag 3180

3121 tagtcttcgg tctgtaattg cgaagacctc tttgagttgc tcgacctgcg cctacttgtc 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg 3240

3181 cgtctgtaga cacttagcga agtgctggtg cgactactcg aaatggcgtc gacggagcgc 3240

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 3300

3241 gcaaagccac tactgccact tttggagact gtgtacgtcg agggcctctg ccagtgtcga 3300

.......... ROP .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 3360

3301 acagacattc gcctacggcc ctcgtctgtt cgggcagtcc cgcgcagtcg cccacaaccg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta 3420

3361 cccacagccc cgcgtcggta ctgggtcagt gcatcgctat cgcctcacat atgaccgaat 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc 3480

3421 tgatacgccg tagtctcgtc taacatgact ctcacgtggt atatacgcca cactttatgg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga 3540

3481 cgtgtctacg cattcctctt ttatggcgta gtccgcgaga aggcgaagga gcgagtgact 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3600

3541 gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3660

3601 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3720

3661 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 3720

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3721 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3780

3721 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3840

3781 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3900

3841 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3960

3901 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4020

3961 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4080

4021 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4140

4081 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4200

4141 ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4260

4201 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4320

4261 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 4380

4321 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 4440

4381 gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt catatatact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4500

4441 catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac 4500

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 4560

4501 agataaagca agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 4620

4561 cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 4680

4621 tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 4740

4681 aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... PstI(4777) .......... ..........

4741 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc 4800

4741 tcaattatca aacgcgttgc aacaacggta acgacgtccg tagcaccaca gtgcgagcag 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4860

4801 caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4920

4861 gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4980

4921 ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5040

4981 taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 5100

5041 atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 5160

5101 gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 5220

5161 gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 5280

5221 tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 5340

5281 tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat 5340

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 5400

5341 aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt 5400

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa 5460

5401 tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac tttaacattt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 5520

5461 gcaattataa aacaatttta agcgcaattt aaaaacaatt tagtcgagta aaaaattggt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 5580

5521 tatccggctt tagccgtttt agggaatatt tagttttctt atctggctct atcccaactc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 5640

5581 acaacaaggt caaaccttgt tctcaggtga taatttcttg cacctgaggt tgcagtttcc 5640

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 5700

5641 cgctttttgg cagatagtcc cgctaccggg tgatgcactt ggtagtggga ttagttcaaa 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 5760

5701 aaaccccagc tccacggcat ttcgtgattt agccttggga tttccctcgg gggctaaatc 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 5820

5761 tcgaactgcc cctttcggcc gcttgcaccg ctctttcctt cccttctttc gctttcctcg 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 5880

5821 cccgcgatcc cgcgaccgtt cacatcgcca gtgcgacgcg cattggtggt gtgggcggcg 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gcttaatgcg ccgctacagg gcgcgtccca ttcgcca... .......... .......... 5917

5881 cgaattacgc ggcgatgtcc cgcgcagggt aagcggt... .......... .......... 5917

f1_origin .......... .......... .......... .......... ..........