*LOCUS pET-32 Xa/LIC 5926 bp DNA SYN 23-Sep-2007 DEFINITION pET-32 Xa/LIC ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5926 /organism="pET-32 Xa/LIC" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(727..743) /label="T7_transl_en_RBS" misc_feature complement(761..788) /label="lacO" promoter complement(788..806) /label="T7_promoter" CDS 353..1447 /label="ORF frame 2" /translation="MMMMMVHMARTRTGQVSVEELFQLTFRQCTHFGCRHFTVFEQQQ SRDTTDAIFRRSARVLIDVQFCNGQFALIFVSDFIQNRGDHFARTAPLCPEIDEDRPV RFEYIRVKTVVSQVNNFIAHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSIS ILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLG AISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRES IQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRG TTTGGQTVVADWRCHLQSGPARAVANCRGD*" misc_feature complement(894..913) /label="pBRrevBam_primer" gene complement(842..1105) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 1188..2279 /label="lacI" CDS 1320..2279 /label="ORF frame 3" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(2338..2613) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 3088..3279 /label="ROP" misc_feature complement(3273..3295) /label="pGEX_3_primer" rep_origin complement(3694..4313) /label="pBR322_origin" CDS complement(4468..5328) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4468..5328) /label="Ampicillin" /gene="Ampicillin" promoter complement(5370..5398) /label="AmpR_promoter" rep_origin complement(5592..5898) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca // pET-32 Xa/LIC "Times New RomanC T7_term"Times New RomanC EWT7_Terminal_pri"Times New RomanCɆ 6xHis"Times New RomanCɆ aORF 2"Times New Roman T7_transl_en_RBS"Times New RomanC lacO"Times New RomanC &T7_pro"Times New Roman*C JQ tet(564-300)"Times New Roman ~ pBRrevBam_pri"Times New Roman*C lacI"Times New RomanCɆ (ORF 3"Times New Roman* " 5  tet(852-576)"Times New RomanC  ROP"Times New RomanC   pGEX_3_pri"Times New RomanCɆ n pBR322_origin"Times New Roman tORF 1"Times New RomanC t Ampicillin"Times New Roman(C AmpR_pro"Times New RomanCɆ   f1_origin"Times New Roman ͓XhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),EcoRV(206),NcoI(211),AgeI(257),KpnI(264),BglII(267),BstBI(294)"Times New Roman k 7UI' XbaI"Times New Roman >ELL BclI"Times New Roman ~ ApaI"Times New Roman t~ HpaI"Times New Roman U&],e1e1@ PstI"Times New Roman LIEu>L test"Times New RomanO .N test"Times New Roman  S0, @{ j