Sequence of pET-32_Xa

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Sequence of pET-32_Xa_LIC

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Sequence of pET-32 Xa/LIC : 5926 BP; 1403 A; 1573 C; 1567 G; 1383 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... ..........

181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct 240

181 gctgcctcga gcttaagcct aggctatagc ggtaccggtc tcctctcaat ctcgctggga 240

.......... .......... .......... .......... .......... ..........

..... AgeI(257) KpnI(264),BglII(267) ..... .......... BstBI(294)

241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt 300

241 gttatggcct cggtggtggc catgggtcta gacccgacag gtacacgacc gcaagcttaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat 360

301 atcgtcgtcg ccaaagaaag tatggtcttg gcgcaccgtg gtctggtctt cttactacta 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca 420

361 ctactaccac gtataccggt cttggtcttg gccggtccaa tcgcagctcc ttgagaaagt 420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca 480

421 tgactggaaa tctgtcacgt gggtgaaacc aacggcggtg aagtggcaaa aacttgtcgt 480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt 540

481 cgtctcagcc ctatggtgcc tacggtataa agccgcgtca cggtcccaaa actagctaca 540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg 600

541 agtcaaaacg ttgccagtca aacgggacta taagcagtcg ctaaagtagg tcttagcccc 600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg 660

601 gctagtaaaa cgtgcctggc gtggtgagac gggtctttag ctgctcctag cggggcaggc 660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat 720

661 gaaactcatg taggcacagt tttgacagca gtcagtccac ttattaaaat agcgagtata 720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... XbaI(755) .......... ..........

721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 780

721 catatagagg aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt 780

....... T7_transl_en_RBS ORF 2 ....... lacO ..........

.......... .......... .......... .......... .......... ..........

781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta 840

781 gttaagggga tatcactcag cataattaaa gcgccctagc tctagctaga gctaggagat 840

.......... T7_pro ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 900

841 gcggcctgcg tagcaccggc cgtagtggcc gcggtgtcca cgccaacgac cgcggatata 900

.......... ......... ORF 2 tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 960

901 gcggctgtag tggctacccc ttctagcccg agcggtgaag cccgagtact cgcgaacaaa 960

pBRrevBam_pri .. ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 1020

961 gccgcaccca taccaccgtc cggggcaccg gccccctgac aacccgcggt agaggaacgt 1020

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 1080

1021 acgtggtaag gaacgccgcc gccacgagtt gccggagttg gatgatgacc cgacgaagga 1080

.......... ...... ORF 2 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa 1140

1081 ttacgtcctc agcgtattcc ctctcgcagc tctagggcct gtggtagctt accgcgtttt 1140

......... tet(564-300) ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 1200

1141 ggaaagcgcc ataccgtact atcgcgggcc ttctctcagt taagtcccac cacttacact 1200

.......... .......... ORF 2 .......... .......... lacI

.......... .......... .......... .......... .......... ..........

1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 1260

1201 ttggtcattg caatatgcta cagcgtctca tacggccaca gagaatagtc tggcaaaggg 1260

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 1320

1261 cgcaccactt ggtccggtcg gtgcaaagac gcttttgcgc cctttttcac cttcgccgct 1320

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 1380

1321 accgcctcga cttaatgtaa gggttggcgc accgtgttgt tgaccgcccg tttgtcagca 1380

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 1440

1381 acgactaacc gcaacggtgg aggtcagacc gggacgtgcg cggcagcgtt taacagcgcc 1440

.......... ....... ORF 2 lacI ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 1500

1441 gctaatttag agcgcggcta gttgacccac ggtcgcacca ccacagctac catcttgctt 1500

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 1560

1501 cgccgcagct tcggacattt cgccgccacg tgttagaaga gcgcgttgcg cagtcacccg 1560

.......... .......... lacI ORF 3 .......... .......... ..........

BclI(1561) .......... .......... .......... .......... ..........

1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 1620

1561 actagtaatt gataggcgac ctactggtcc tacggtaacg acaccttcga cggacgtgat 1620

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 1680

1621 tacaaggccg caataaagaa ctacagagac tggtctgtgg gtagttgtca taataaaaga 1680

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 1740

1681 gggtacttct gccatgcgct gacccgcacc tcgtagacca gcgtaaccca gtggtcgttt 1740

.......... .......... lacI ORF 3 .......... .......... ..........

.......... ApaI(1758) .......... .......... .......... ..........

1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 1800

1741 agcgcgacaa tcgcccgggt aattcaagac agagccgcgc agacgcagac cgaccgaccg 1800

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 1860

1801 tatttataga gtgagcgtta gtttaagtcg gctatcgcct tgcccttccg ctgacctcac 1860

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 1920

1861 ggtacaggcc aaaagttgtt tggtacgttt acgacttact cccgtagcaa gggtgacgct 1920

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 1980

1921 acgaccaacg gttgctagtc taccgcgacc cgcgttacgc gcggtaatgg ctcaggcccg 1980

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 2040

1981 acgcgcaacc acgcctgtag agccatcacc ctatgctgct atggcttctg tcgagtacaa 2040

.......... .......... lacI ORF 3 .......... .......... ..........

.......... HpaI(2053) .......... .......... .......... ..........

2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 2100

2041 tatagggcgg caattggtgg tagtttgtcc taaaagcgga cgaccccgtt tggtcgcacc 2100

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 2160

2101 tggcgaacga cgttgagaga gtcccggtcc gccacttccc gttagtcgac aacgggcaga 2160

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 2220

2161 gtgaccactt ttctttttgg tgggaccgcg ggttatgcgt ttggcggaga ggggcgcgca 2220

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 2280

2221 accggctaag taattacgtc gaccgtgctg tccaaagggc tgacctttcg cccgtcactc 2280

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc 2340

2281 gcgttgcgtt aattacattc aatcgagtga gtaatccgtg gccctagagc tggctacggg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 2400

2341 aactctcgga agttgggtca gtcgaggaag gccacccgcg ccccgtactg atagcagcgg 2400

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg 2460

2401 cgtgaatact gacagaagaa atagtacgtt gagcatcctg tccacggccg tcgcgagacc 2460

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg 2520

2461 cagtaaaagc cgctcctggc gaaagcgacc tcgcgctgct actagccgga cagcgaacgc 2520

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt 2580

2521 cataagcctt agaacgtgcg ggagcgagtt cggaagcagt gaccagggcg gtggtttgca 2580

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg 2640

2581 aagccgctct tcgtccggta atagcggccg taccgccggg gtgcccacgc gtactagcac 2640

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa 2700

2641 gaggacagca actcctgggc cgatccgacc gccccaacgg aatgaccaat cgtcttactt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca 2760

2701 agtggctatg cgctcgcttg cacttcgctg acgacgacgt tttgcagacg ctggactcgt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg 2820

2761 tgttgtactt accagaagcc aaaggcacaa agcatttcag acctttgcgc cttcagtcgc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca 2880

2821 gggacgtggt aatacaaggc ctagacgtag cgtcctacga cgaccgatgg gacaccttgt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc 2940

2881 ggatgtagac ataattgctt cgcgaccgta actgggactc actaaaaaga gaccagggcg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca 3000

2941 gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt cattggcccg tacaagtagt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac 3060

3001 agtcattggg catagcactc gtaggagaga gcaaagtagc catagtaatg ggggtacttg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg 3120

3061 tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc ttttttggcg ggaattgtac 3120

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg 3180

3121 cgggcgaaat agtcttcggt ctgtaattgc gaagacctct ttgagttgct cgacctgcgc 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc 3240

3181 ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc gactactcga aatggcgtcg 3240

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 3300

3241 acggagcgcg caaagccact actgccactt ttggagactg tgtacgtcga gggcctctgc 3300

.......... ROP .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 3360

3301 cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc gcgcagtcgc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 3420

3361 ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg catcgctatc gcctcacata 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg 3480

3421 tgaccgaatt gatacgccgt agtctcgtct aacatgactc tcacgtggta tatacgccac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 3540

3481 actttatggc gtgtctacgc attcctcttt tatggcgtag tccgcgagaa ggcgaaggag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3600

3541 cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3660

3601 ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 3720

3661 tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga 3720

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 3780

3721 ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 3840

3781 tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 3900

3841 ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 3960

3901 agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4020

3961 acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4080

4021 caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4140

4081 gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4200

4141 gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4260

4201 tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4320

4261 gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4380

4321 ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4440

4381 tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4500

4441 atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag 4500

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4560

4501 tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4620

4561 ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4680

4621 tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 4740

4681 aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(4786) ..........

4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 4800

4741 atcaagcggt caattatcaa acgcgttgca acaacggtaa cgacgtccgt agcaccacag 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 4860

4801 tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 4920

4861 tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 4980

4921 ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5040

4981 acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5100

5041 tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5160

5101 cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5220

5161 gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5280

5221 tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5340

5281 acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5400

5341 agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac 5400

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5460

5401 ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5520

5461 ttaacatttg caattataaa acaattttaa gcgcaattta aaaacaattt agtcgagtaa 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5580

5521 aaaattggtt atccggcttt agccgtttta gggaatattt agttttctta tctggctcta 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5640

5581 tcccaactca caacaaggtc aaaccttgtt ctcaggtgat aatttcttgc acctgaggtt 5640

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5700

5641 gcagtttccc gctttttggc agatagtccc gctaccgggt gatgcacttg gtagtgggat 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5760

5701 tagttcaaaa aaccccagct ccacggcatt tcgtgattta gccttgggat ttccctcggg 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 5820

5761 ggctaaatct cgaactgccc ctttcggccg cttgcaccgc tctttccttc ccttctttcg 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 5880

5821 ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag tgcgacgcgc attggtggtg 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca.... .......... 5926

5881 tgggcggcgc gaattacgcg gcgatgtccc gcgcagggta agcggt.... .......... 5926

f1_origin .......... .......... .......... .......... ..........