Sequence of pET-32_a_b

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Sequence of pET-32_a_b_c(+)

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Sequence of pET-32 a,b,c(+) : 5900 BP; 1391 A; 1564 C; 1560 G; 1385 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI0),EcoRI(192) BamHI(198) EcoRV(206) NcoI(212) ........

181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc 240

181 gctgcctcga gcttaagcct aggctatagt cggtaccgga acagcagcag cagccatggg 240

.......... .......... .......... .......... EK ..........

BglII(241) .......... BstBI(268) .......... .......... ..........

241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca 300

241 tctagacccg acaggtacac gaccgcaagc ttaaatcgtc gtcgccaaag aaagtatggt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(351)

301 gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg gccagaacca 360

301 cttggcgcac cgtggtctgg tcttcttact actactacta ccacgtatac cggtcttggt 360

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

361 gaaccggcca ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcacccact 420

361 cttggccggt ccaatcgcag ctccttgaga aagttgactg gaaatctgtc acgtgggtga 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca 480

421 aaccaacggc ggtgaagtgg caaaaacttg tcgtcgtctc agccctatgg tgcctacggt 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc 540

481 ataaagccgc gtcacggtcc caaaactagc tacaagtcaa aacgttgcca gtcaaacggg 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac 600

541 actataagca gtcgctaaag taggtcttag ccccgctagt aaaacgtgcc tggcgtggtg 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg 660

601 agacgggtct ttagctgctc ctagcggggc aggcgaaact catgtaggca cagttttgac 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcgtcagtca ggtgaataat tttatcgctc atatgtatat ctccttctta aagttaaaca 720

661 agcagtcagt ccacttatta aaatagcgag tatacatata gaggaagaat ttcaatttgt 720

.......... .......... ORF 3 ....... T7_transl_en_RBS .......

XbaI(729) .......... .......... .......... .......... ..........

721 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta 780

721 tttaataaag atctcccctt aacaataggc gagtgttaag gggatatcac tcagcataat 780

.......... .......... ORF 3 lacO .......... .......... T7_pro

.......... .......... .......... .......... .......... ..........

781 atttcgcggg atcgagatcg atctcgatcc tctacgccgg acgcatcgtg gccggcatca 840

781 taaagcgccc tagctctagc tagagctagg agatgcggcc tgcgtagcac cggccgtagt 840

.......... .......... ORF 3 ......... tet(564-300) .........

.......... .......... .......... .......... .......... ..........

841 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 900

841 ggccgcggtg tccacgccaa cgaccgcgga tatagcggct gtagtggcta ccccttctag 900

.......... .... ORF 3 tet(564-300) pBRrevBam_pri .... ..........

.......... .......... .......... .......... .......... ..........

901 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 960

901 cccgagcggt gaagcccgag tactcgcgaa caaagccgca cccataccac cgtccggggc 960

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 1020

961 accggccccc tgacaacccg cggtagagga acgtacgtgg taaggaacgc cgccgccacg 1020

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 1080

1021 agttgccgga gttggatgat gacccgacga aggattacgt cctcagcgta ttccctctcg 1080

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc 1140

1081 cagctctagg gcctgtggta gcttaccgcg ttttggaaag cgccataccg tactatcgcg 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 1200

1141 ggccttctct cagttaagtc ccaccactta cactttggtc attgcaatat gctacagcgt 1200

.......... .......... ORF 3 .......... lacI ..........

.......... .......... .......... .......... .......... ..........

1201 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 1260

1201 ctcatacggc cacagagaat agtctggcaa agggcgcacc acttggtccg gtcggtgcaa 1260

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 1320

1261 agacgctttt gcgccctttt tcaccttcgc cgctaccgcc tcgacttaat gtaagggttg 1320

.......... .......... ORF 3 lacI .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1321 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 1380

1321 gcgcaccgtg ttgttgaccg cccgtttgtc agcaacgact aaccgcaacg gtggaggtca 1380

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 1440

1381 gaccgggacg tgcgcggcag cgtttaacag cgccgctaat ttagagcgcg gctagttgac 1440

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 1500

1441 ccacggtcgc accaccacag ctaccatctt gcttcgccgc agcttcggac atttcgccgc 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... BclI(1535) .......... ..........

1501 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 1560

1501 cacgtgttag aagagcgcgt tgcgcagtca cccgactagt aattgatagg cgacctactg 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 1620

1561 gtcctacggt aacgacacct tcgacggacg tgattacaag gccgcaataa agaactacag 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 1680

1621 agactggtct gtgggtagtt gtcataataa aagagggtac ttctgccatg cgctgacccg 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(1732)

1681 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 1740

1681 cacctcgtag accagcgtaa cccagtggtc gtttagcgcg acaatcgccc gggtaattca 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 1800

1741 agacagagcc gcgcagacgc agaccgaccg accgtattta tagagtgagc gttagtttaa 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 1860

1801 gtcggctatc gccttgccct tccgctgacc tcacggtaca ggccaaaagt tgtttggtac 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 1920

1861 gtttacgact tactcccgta gcaagggtga cgctacgacc aacggttgct agtctaccgc 1920

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga catctcggta 1980

1921 gacccgcgtt acgcgcggta atggctcagg cccgacgcgc aaccacgcct gtagagccat 1980

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... HpaI(2027) ..........

1981 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 2040

1981 caccctatgc tgctatggct tctgtcgagt acaatatagg gcggcaattg gtggtagttt 2040

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 2100

2041 gtcctaaaag cggacgaccc cgtttggtcg cacctggcga acgacgttga gagagtcccg 2100

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 2160

2101 gtccgccact tcccgttagt cgacaacggg cagagtgacc acttttcttt ttggtgggac 2160

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2220

2161 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 2220

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct 2280

2221 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattac attcaatcga 2280

.......... lacI ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc 2340

2281 gtgagtaatc cgtggcccta gagctggcta cgggaactct cggaagttgg gtcagtcgag 2340

.......... .......... .......... ......... tet(852-576) .........

.......... .......... .......... .......... .......... ..........

2341 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 2400

2341 gaaggccacc cgcgccccgt actgatagca gcggcgtgaa tactgacaga agaaatagta 2400

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 2460

2401 cgttgagcat cctgtccacg gccgtcgcga gacccagtaa aagccgctcc tggcgaaagc 2460

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 2520

2461 gacctcgcgc tgctactagc cggacagcga acgccataag ccttagaacg tgcgggagcg 2520

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 2580

2521 agttcggaag cagtgaccag ggcggtggtt tgcaaagccg ctcttcgtcc ggtaatagcg 2580

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 2640

2581 gccgtaccgc cggggtgccc acgcgtacta gcacgaggac agcaactcct gggccgatcc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 2700

2641 gaccgcccca acggaatgac caatcgtctt acttagtggc tatgcgctcg cttgcacttc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 2760

2701 gctgacgacg acgttttgca gacgctggac tcgttgttgt acttaccaga agccaaaggc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg 2820

2761 acaaagcatt tcagaccttt gcgccttcag tcgcgggacg tggtaataca aggcctagac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg 2880

2821 gtagcgtcct acgacgaccg atgggacacc ttgtggatgt agacataatt gcttcgcgac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc 2940

2881 cgtaactggg actcactaaa aagagaccag ggcggcgtag gtatggcggt caacaaatgg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct 3000

2941 gagtgttgca aggtcattgg cccgtacaag tagtagtcat tgggcatagc actcgtagga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc 3060

3001 gagagcaaag tagccatagt aatgggggta cttgtcttta gggggaatgt gcctccgtag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 3120

3061 tcactggttt gtcctttttt ggcgggaatt gtaccgggcg aaatagtctt cggtctgtaa 3120

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 3180

3121 ttgcgaagac ctctttgagt tgctcgacct gcgcctactt gtccgtctgt agacacttag 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg 3240

3181 cgaagtgctg gtgcgactac tcgaaatggc gtcgacggag cgcgcaaagc cactactgcc 3240

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 3300

3241 acttttggag actgtgtacg tcgagggcct ctgccagtgt cgaacagaca ttcgcctacg 3300

ROP pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 3360

3301 gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa ccgcccacag ccccgcgtcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 3420

3361 gtactgggtc agtgcatcgc tatcgcctca catatgaccg aattgatacg ccgtagtctc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga 3480

3421 gtctaacatg actctcacgt ggtatatacg ccacacttta tggcgtgtct acgcattcct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3540

3481 cttttatggc gtagtccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3600

3541 aagccgacgc cgctcgccat agtcgagtga gtttccgcca ttatgccaat aggtgtctta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3660

3601 gtcccctatt gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3720

3661 ttttccggcg caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt 3720

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 3780

3721 tagctgcgag ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 3840

3781 ggggaccttc gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 3900

3841 ggcggaaaga gggaagccct tcgcaccgcg aaagagtatc gagtgcgaca tccatagagt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 3960

3901 caagccacat ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4020

3961 tggcgacgcg gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4080

4021 gcggtgaccg tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4140

4081 gtctcaagaa cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4200

4141 cgcgagacga cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4260

4201 tttggtggcg accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4320

4261 ttcctagagt tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt 4320

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4380

4321 tgagtgcaat tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4440

4381 atttaatttt tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4500

4441 caatggttac gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt 4500

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4560

4501 atcaacggac tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4620

4561 ggtcacgacg ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4680

4621 tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4740

4681 tcagataatt aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... PstI(4760) .......... .......... .......... ..........

4741 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 4800

4741 tgcaacaacg gtaacgacgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 4860

4801 agtcgaggcc aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 4920

4861 gccaatcgag gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 4980

4921 agtaccaata ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5040

4981 gacactgacc actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5100

5041 cgagaacggg ccgcagttat gccctattat ggcgcggtgt atcgtcttga aattttcacg 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5160

5101 agtagtaacc ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5220

5161 ggtcaagcta cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5280

5221 cgcaaagacc cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5340

5281 gtgcctttac aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc 5340

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5400

5341 caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc 5400

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 5460

5401 aaggcgcgtg taaaggggct tttcacggtg gactttaaca tttgcaatta taaaacaatt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 5520

5461 ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg ctttagccgt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 5580

5521 tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa ggtcaaacct 5580

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

5581 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 5640

5581 tgttctcagg tgataatttc ttgcacctga ggttgcagtt tcccgctttt tggcagatag 5640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 5700

5641 tcccgctacc gggtgatgca cttggtagtg ggattagttc aaaaaacccc agctccacgg 5700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5760

5701 catttcgtga tttagccttg ggatttccct cgggggctaa atctcgaact gcccctttcg 5760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5820

5761 gccgcttgca ccgctctttc cttcccttct ttcgctttcc tcgcccgcga tcccgcgacc 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5880

5821 gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 agggcgcgtc ccattcgcca .......... .......... .......... .......... 5900

5881 tcccgcgcag ggtaagcggt .......... .......... .......... .......... 5900

.......... .......... .......... .......... .......... ..........