%LOCUS pET-33b(+) 5383 bp DNA SYN 14-Sep-2007 DEFINITION pET-33b(+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5383 /organism="pET-33b(+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(140..157) /label="6xHis" misc_feature complement(206..238) /label="T7_gene10_leader" misc_feature complement(206..238) /label="T7_leader" misc_feature complement(221..239) /label="Xpress_fwd_primer" misc_feature complement(321..337) /label="T7_transl_en_RBS" misc_feature complement(355..382) /label="lacO" promoter complement(382..400) /label="T7_promoter" misc_feature complement(484..503) /label="pBRrevBam_primer" gene complement(432..695) /label="tet(564-300)" /gene="tet(564-300)" misc_feature 778..1869 /label="lacI" CDS 910..1869 /label="ORF frame 1" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene complement(1928..2203) /label="tet(852-576)" /gene="tet(852-576)" misc_feature 2678..2869 /label="ROP" misc_feature complement(2863..2885) /label="pGEX_3_primer" rep_origin complement(3284..3903) /label="pBR322_origin" CDS 4009..4824 /label="KanR2" /gene="KanR2" /note="ORF frame 1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" gene 4009..4824 /label="KanR2" /gene="KanR2" rep_origin complement(5049..5355) /label="f1_origin" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat 301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa 361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct 421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa 721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg 781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag 841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg 901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc 961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa 1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg 1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc 1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct 1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt 1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt 1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg 1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc 1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt 1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc 1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac 1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa 1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg 1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct 1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg 1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg 2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat 4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat 4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa 4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat 4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat 4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca //  pET-33b(+) "Times New RomanCT7_term"Times New RomanCEWT7_Terminal_pri"Times New RomanCɆ6xHis"Times New RomanT7_gene10_leader"Times New RomanC T7_leader"Times New RomanCXpress_fwd_pri"Times New Roman*CɆAQT7_transl_en_RBS"Times New Romanc~lacO"Times New RomanC~T7_pro"Times New RomanCɆ tet(564-300)"Times New RomanC pBRrevBam_pri"Times New Roman MlacI"Times New RomanCMORF 1"Times New RomanC tet(852-576)"Times New RomanCɆv 5 ROP"Times New Roman/ E  pGEX_3_pri"Times New RomanC ? pBR322_origin"Times New RomanCORF 1"Times New RomanCɆKanR2"Times New Roman f1_origin"Times New Roman hXhoI(158),NotI(166),EagI(166),HindIII(173),SalI(179),SacI(190),EcoRI(192),BamHI(198),NheI(230),NdeI(237)"Times New Roman }b NcoI(310),XbaI(349),BglII(415)"Times New Roman699A BclI"Times New Roman ApaI"Times New RomanD EcoRV(1587),HpaI(1643)"Times New Roman3   FspI"Times New Roman %**h NruI(4097),ClaI(4131)"Times New Romanh SmaI"Times New Romans} test"Times New RomanXZ) test"Times New Roman3-399