Sequence of pET-33b(+)

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Sequence of pET-33b(+)

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Sequence of pET-33b(+) : 5383 BP; 1274 A; 1395 C; 1447 G; 1267 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_term .......... 6xHis .......... .......... ..........

SacI90),EcoRI(192) BamHI(198) ........ NheI(230) NdeI(237)

181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 240

181 gctgcctcga gcttaagcct agggctgggt aaacgacagg tggtcagtac gatcggtata 240

.......... .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat 300

241 cttgtctacg tgctgccgac ggcgcgccgt ggtccggcga cgacactact actactacta 300

.......... .......... .......... .......... .......... ..........

NcoI(310) .......... .......... .......... .......... ..........

301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa 360

301 ccgacgacgg gtaccatata gaggaagaat ttcaatttgt tttaataaag atctcccctt 360

.......... ....... T7_transl_en_RBS ....... .......... ..........

.......... .......... .......... .......... .......... BglII(415)

361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct 420

361 aacaataggc gagtgttaag gggatatcac tcagcataat taaagcgccc tagctctaga 420

.......... lacO .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 480

421 gctaggagat gcggcctgcg tagcaccggc cgtagtggcc gcggtgtcca cgccaacgac 480

.......... .......... ......... tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 540

481 cgcggatata gcggctgtag tggctacccc ttctagcccg agcggtgaag cccgagtact 540

....... pBRrevBam_pri tet(564-300) ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 600

541 cgcgaacaaa gccgcaccca taccaccgtc cggggcaccg gccccctgac aacccgcggt 600

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 660

601 agaggaacgt acgtggtaag gaacgccgcc gccacgagtt gccggagttg gatgatgacc 660

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa 720

661 cgacgaagga ttacgtcctc agcgtattcc ctctcgcagc tctagggcct gtggtagctt 720

......... tet(564-300) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg 780

721 accgcgtttt ggaaagcgcc ataccgtact atcgcgggcc ttctctcagt taagtcccac 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag 840

781 cacttacact ttggtcattg caatatgcta cagcgtctca tacggccaca gagaatagtc 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg 900

841 tggcaaaggg cgcaccactt ggtccggtcg gtgcaaagac gcttttgcgc cctttttcac 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc 960

901 cttcgccgct accgcctcga cttaatgtaa gggttggcgc accgtgttgt tgaccgcccg 960

.......... .......... lacI ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa 1020

961 tttgtcagca acgactaacc gcaacggtgg aggtcagacc gggacgtgcg cggcagcgtt 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg 1080

1021 taacagcgcc gctaatttag agcgcggcta gttgacccac ggtcgcacca ccacagctac 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc 1140

1081 catcttgctt cgccgcagct tcggacattt cgccgccacg tgttagaaga gcgcgttgcg 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... BclI(1151) .......... .......... .......... ..........

1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct 1200

1141 cagtcacccg actagtaatt gataggcgac ctactggtcc tacggtaacg acaccttcga 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt 1260

1201 cggacgtgat tacaaggccg caataaagaa ctacagagac tggtctgtgg gtagttgtca 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt 1320

1261 taataaaaga gggtacttct gccatgcgct gacccgcacc tcgtagacca gcgtaaccca 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... ApaI(1348) .......... .......... ..........

1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg 1380

1321 gtggtcgttt agcgcgacaa tcgcccgggt aattcaagac agagccgcgc agacgcagac 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc 1440

1381 cgaccgaccg tatttataga gtgagcgtta gtttaagtcg gctatcgcct tgcccttccg 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt 1500

1441 ctgacctcac ggtacaggcc aaaagttgtt tggtacgttt acgacttact cccgtagcaa 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc 1560

1501 gggtgacgct acgaccaacg gttgctagtc taccgcgacc cgcgttacgc gcggtaatgg 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac 1620

1561 ctcaggcccg acgcgcaacc acgcctatag agccatcacc ctatgctgct atggcttctg 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... HpaI(1643) .......... .......... ..........

1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa 1680

1621 tcgagtacaa tatagggcgg caattggtgg tagtttgtcc taaaagcgga cgaccccgtt 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg 1740

1681 tggtcgcacc tggcgaacga cgttgagaga gtcccggtcc gccacttccc gttagtcgac 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct 1800

1741 aacgggcaga gtgaccactt ttctttttgg tgggaccgcg ggttatgcgt ttggcggaga 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1860

1801 ggggcgcgca accggctaag taattacgtc gaccgtgctg tccaaagggc tgacctttcg 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg 1920

1861 cccgtcactc gcgttgcgtt aattacattc aatcgagtga gtaatccgtg gccctagagc 1920

lacI ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 1980

1921 tggctacggg aactctcgga agttgggtca gtcgaggaag gccacccgcg ccccgtactg 1980

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 2040

1981 atagcagcgg cgtgaatact gacagaagaa atagtacgtt gagcatcctg tccacggccg 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 2100

2041 tcgcgagacc cagtaaaagc cgctcctggc gaaagcgacc tcgcgctgct actagccgga 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 2160

2101 cagcgaacgc cataagcctt agaacgtgcg ggagcgagtt cggaagcagt gaccagggcg 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... FspI(2219)

2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg 2220

2161 gtggtttgca aagccgctct tcgtccggta atagcggccg taccgccggg gtgcccacgc 2220

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 2280

2221 gtactagcac gaggacagca actcctgggc cgatccgacc gccccaacgg aatgaccaat 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 2340

2281 cgtcttactt agtggctatg cgctcgcttg cacttcgctg acgacgacgt tttgcagacg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 2400

2341 ctggactcgt tgttgtactt accagaagcc aaaggcacaa agcatttcag acctttgcgc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 2460

2401 cttcagtcgc gggacgtggt aatacaaggc ctagacgtag cgtcctacga cgaccgatgg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 2520

2461 gacaccttgt ggatgtagac ataattgctt cgcgaccgta actgggactc actaaaaaga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 2580

2521 gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt cattggcccg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 2640

2581 tacaagtagt agtcattggg catagcactc gtaggagaga gcaaagtagc catagtaatg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 2700

2641 ggggtacttg tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc ttttttggcg 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 2760

2701 ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc gaagacctct ttgagttgct 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 2820

2761 cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc gactactcga 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2880

2821 aatggcgtcg acggagcgcg caaagccact actgccactt ttggagactg tgtacgtcga 2880

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2940

2881 gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc gggcagtccc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 3000

2941 gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg catcgctatc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 3060

3001 gcctcacata tgaccgaatt gatacgccgt agtctcgtct aacatgactc tcacgtggta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 3120

3061 tatacgccac actttatggc gtgtctacgc attcctcttt tatggcgtag tccgcgagaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3180

3121 ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3240

3181 gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3300

3241 acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa 3300

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3360

3301 aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3420

3361 ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3480

3421 gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3540

3481 accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3600

3541 tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3660

3601 tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3720

3661 tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3780

3721 tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3840

3781 agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3900

3841 aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3960

3901 agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 4020

3961 acttgttatt ttgacagacg aatgtatttg tcattatgtt ccccacaata ctcggtataa 4020

.......... .......... .......... .......... ......... ORF 1 KanR2

.......... .......... .......... .......... .......... ..........

4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat 4080

4021 gttgcccttt gcagaacgag atccggcgct aatttaaggt tgtacctacg actaaatata 4080

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... NruI(4097) .......... .......... .......... ClaI(4131)

4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat 4140

4081 cccatattta cccgagcgct attacagccc gttagtccac gctgttagat agctaacata 4140

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 4200

4141 cccttcgggc tacgcggtct caacaaagac tttgtaccgt ttccatcgca acggttacta 4200

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 4260

4201 caatgtctac tctaccagtc tgatttgacc gactgcctta aatacggaga aggctggtag 4260

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... SmaI(4314)

4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa 4320

4261 ttcgtaaaat aggcatgagg actactacgt accaatgagt ggtgacgcta ggggcccttt 4320

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 4380

4321 tgtcgtaagg tccataatct tcttatagga ctaagtccac ttttataaca actacgcgac 4380

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 4440

4381 cgtcacaagg acgcggccaa cgtaagctaa ggacaaacat taacaggaaa attgtcgcta 4440

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 4500

4441 gcgcataaag cagagcgagt ccgcgttagt gcttacttat tgccaaacca actacgctca 4500

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 4560

4501 ctaaaactac tgctcgcatt accgaccgga caacttgttc agacctttct ttacgtattt 4560

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 4620

4561 gaaaacggta agagtggcct aagtcagcag tgagtaccac taaagagtga actattggaa 4620

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 4680

4621 taaaaactgc tcccctttaa ttatccaaca taactacaac ctgctcagcc ttagcgtctg 4680

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 4740

4681 gctatggtcc tagaacggta ggataccttg acggagccac tcaaaagagg aagtaatgtc 4740

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 4800

4741 tttgccgaaa aagtttttat accataacta ttaggactat acttatttaa cgtcaaagta 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat 4860

4801 aactacgagc tactcaaaaa gattcttaat taagtactcg cctatgtata aacttacata 4860

......... ORF 1 KanR2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat 4920

4861 aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg gtggacttta 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 4980

4921 acatttgcaa ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 5040

4981 attggttatc cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 5100

5041 caactcacaa caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca 5100

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 5160

5101 gtttcccgct ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 5220

5161 ttcaaaaaac cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 5280

5221 taaatctcga actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 5340

5281 tcctcgcccg cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca....... .......... 5383

5341 gcggcgcgaa ttacgcggcg atgtcccgcg cagggtaagc ggt....... .......... 5383

f1_origin .......... .......... .......... .......... ..........