Sequence of pET-39b(+)

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Sequence of pET-39b(+)

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Sequence of pET-39b(+) : 6106 BP; 1456 A; 1587 C; 1598 G; 1465 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 180

121 acaaatcgtc ggatccataa ttagttaatc accaccacca ccaccaccac cacgagctca 180

.......... .......... ...... 6xHis ORF 3 6xHis 6xHis ......

NotI,EagI HindIII SalI SacI,EcoRI(208) BamHI(214)

181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag 240

181 cgccggcgtt cgaacagctg cctcgagctt aagcctaggc tatagcggta ccaactcctc 240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt 300

241 ttcgggcccg agaacagcag cagtagccat gggtctagac ccgacaggta cacgaccgca 300

.......... EK ORF 3 .......... .......... ..........

BstBI(301) .......... .......... .......... .......... ..........

301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga 360

301 agcttaaatc gtcgtcgcca aagaaagtat ggttaacgtc atgatggcgc accgtggtct 360

.......... .......... ORF 3 .......... .......... ..........

SacII(365) .......... .......... SpeI(396) .......... AflII(418)

361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt 420

361 gggcgcctca ctaccactac cactactggt cttggtgatc aactaggaaa aaagagcgaa 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata 480

421 ttcataaagt gacatagtcg tatgacgact tgtttttgta ggtataacga ccataggtat 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc 540

481 gggacgccta agtcgactat aaatggcaat tgtttgtagc ggccttgcgg tgcgttaacg 540

.......... .......... ORF 3 .......... .......... ..........

.......... PstI(552) .......... .......... .......... ..........

541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac 600

541 tgcagtcgac gtcgaaaaag gacgactcgc tggtctctaa agtggtgctt cgacaaggtg 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa 660

601 cggcgcagca tgagaagtgg aaattatgga cgcaactatt tatgtagcgc ctatagtctt 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct 720

661 cgtcttgctt accagaccca aaagacatgc ggaagtttgt cgccttgtca gtgaaacaga 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca 780

721 aggtgcgggt cgcggtagcg gtgtcgggta cggactcagt ctagaaacgg gtccagtggt 780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca 840

781 gggtacttca actgcaccat aaatcagtag aagtgcggaa ggccgtcaaa aaagaagtgt 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa 900

841 aatagtcttt atacgtcttg aagaagtttg actatcgtca cgcccgtctt ctttctcttt 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca 960

901 ttgaggtcgt gaacgccgcg cggtcgatgg ccaaaaaggt cccatcacat gacaaatggt 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa 1020

961 agaagtatga cgcggcggct acgcgatttg cgattttgat ttggtcggtc gcggtcggtt 1020

.......... .......... ORF 3 .......... .......... ..........

NdeI(1030) .......... .......... .......... XbaI(1068) ..........

1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 1080

1021 tagaaaaagt atacatatag aggaagaatt tcaatttgtt ttaataaaga tctcccctta 1080

ORF 3 ....... T7_transl_en_RBS ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga 1140

1081 acaataggcg agtgttaagg ggatatcact cagcataatt aaagcgccct agctctagct 1140

......... lacO ORF 3 T7_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 1200

1141 agagctagga gatgcggcct gcgtagcacc ggccgtagtg gccgcggtgt ccacgccaac 1200

.......... ......... ORF 3 tet(564-300) ......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 1260

1201 gaccgcggat atagcggctg tagtggctac cccttctagc ccgagcggtg aagcccgagt 1260

.... pBRrevBam_pri ORF 3 tet(564-300) .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 1320

1261 actcgcgaac aaagccgcac ccataccacc gtccggggca ccggccccct gacaacccgc 1320

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 1380

1321 ggtagaggaa cgtacgtggt aaggaacgcc gccgccacga gttgccggag ttggatgatg 1380

.......... ...... ORF 3 tet(564-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc 1440

1381 acccgacgaa ggattacgtc ctcagcgtat tccctctcgc agctctaggg cctgtggtag 1440

......... tet(564-300) ORF 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 1500

1441 cttaccgcgt tttggaaagc gccataccgt actatcgcgg gccttctctc agttaagtcc 1500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 1560

1501 caccacttac actttggtca ttgcaatatg ctacagcgtc tcatacggcc acagagaata 1560

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 1620

1561 gtctggcaaa gggcgcacca cttggtccgg tcggtgcaaa gacgcttttg cgcccttttt 1620

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 1680

1621 caccttcgcc gctaccgcct cgacttaatg taagggttgg cgcaccgtgt tgttgaccgc 1680

.......... .......... ORF 3 lacI ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 1740

1681 ccgtttgtca gcaacgacta accgcaacgg tggaggtcag accgggacgt gcgcggcagc 1740

.......... ....... ORF 3 lacI ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 1800

1741 gtttaacagc gccgctaatt tagagcgcgg ctagttgacc cacggtcgca ccaccacagc 1800

ORF 3 .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 1860

1801 taccatcttg cttcgccgca gcttcggaca tttcgccgcc acgtgttaga agagcgcgtt 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... BclI(1874) .......... .......... .......... ..........

1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 1920

1861 gcgcagtcac ccgactagta attgataggc gacctactgg tcctacggta acgacacctt 1920

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 1980

1921 cgacggacgt gattacaagg ccgcaataaa gaactacaga gactggtctg tgggtagttg 1980

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 2040

1981 tcataataaa agagggtact tctgccatgc gctgacccgc acctcgtaga ccagcgtaac 2040

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... ApaI(2071) .......... ..........

2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 2100

2041 ccagtggtcg tttagcgcga caatcgcccg ggtaattcaa gacagagccg cgcagacgca 2100

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 2160

2101 gaccgaccga ccgtatttat agagtgagcg ttagtttaag tcggctatcg ccttgccctt 2160

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 2220

2161 ccgctgacct cacggtacag gccaaaagtt gtttggtacg tttacgactt actcccgtag 2220

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 2280

2221 caagggtgac gctacgacca acggttgcta gtctaccgcg acccgcgtta cgcgcggtaa 2280

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa 2340

2281 tggctcaggc ccgacgcgca accacgcctg tagagccatc accctatgct gctatggctt 2340

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 2400

2341 ctgtcgagta caatataggg cggcaattgg tggtagtttg tcctaaaagc ggacgacccc 2400

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 2460

2401 gtttggtcgc acctggcgaa cgacgttgag agagtcccgg tccgccactt cccgttagtc 2460

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 2520

2461 gacaacgggc agagtgacca cttttctttt tggtgggacc gcgggttatg cgtttggcgg 2520

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 2580

2521 agaggggcgc gcaaccggct aagtaattac gtcgaccgtg ctgtccaaag ggctgacctt 2580

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc 2640

2581 tcgcccgtca ctcgcgttgc gttaattaca ttcaatcgag tgagtaatcc gtggccctag 2640

lacI ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 2700

2641 agctggctac gggaactctc ggaagttggg tcagtcgagg aaggccaccc gcgccccgta 2700

.......... .......... ......... tet(852-576) ......... ..........

.......... .......... .......... .......... .......... ..........

2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 2760

2701 ctgatagcag cggcgtgaat actgacagaa gaaatagtac gttgagcatc ctgtccacgg 2760

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 2820

2761 ccgtcgcgag acccagtaaa agccgctcct ggcgaaagcg acctcgcgct gctactagcc 2820

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc 2880

2821 ggacagcgaa cgccataagc cttagaacgt gcgggagcga gttcggaagc agtgaccagg 2880

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt 2940

2881 gcggtggttt gcaaagccgc tcttcgtccg gtaatagcgg ccgtaccgcc ggggtgccca 2940

.......... ......... tet(852-576) ......... .......... ..........

FspI(2942) .......... .......... .......... .......... ..........

2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 3000

2941 cgcgtactag cacgaggaca gcaactcctg ggccgatccg accgccccaa cggaatgacc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 3060

3001 aatcgtctta cttagtggct atgcgctcgc ttgcacttcg ctgacgacga cgttttgcag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 3120

3061 acgctggact cgttgttgta cttaccagaa gccaaaggca caaagcattt cagacctttg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct 3180

3121 cgccttcagt cgcgggacgt ggtaatacaa ggcctagacg tagcgtccta cgacgaccga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt 3240

3181 tgggacacct tgtggatgta gacataattg cttcgcgacc gtaactggga ctcactaaaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg 3300

3241 agagaccagg gcggcgtagg tatggcggtc aacaaatggg agtgttgcaa ggtcattggc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat 3360

3301 ccgtacaagt agtagtcatt gggcatagca ctcgtaggag agagcaaagt agccatagta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac 3420

3361 atgggggtac ttgtctttag ggggaatgtg cctccgtagt cactggtttg tccttttttg 3420

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa 3480

3421 gcgggaattg taccgggcga aatagtcttc ggtctgtaat tgcgaagacc tctttgagtt 3480

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga 3540

3481 gctcgacctg cgcctacttg tccgtctgta gacacttagc gaagtgctgg tgcgactact 3540

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 3600

3541 cgaaatggcg tcgacggagc gcgcaaagcc actactgcca cttttggaga ctgtgtacgt 3600

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 3660

3601 cgagggcctc tgccagtgtc gaacagacat tcgcctacgg ccctcgtctg ttcgggcagt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 3720

3661 cccgcgcagt cgcccacaac cgcccacagc cccgcgtcgg tactgggtca gtgcatcgct 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 3780

3721 atcgcctcac atatgaccga attgatacgc cgtagtctcg tctaacatga ctctcacgtg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 3840

3781 gtatatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgcga 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 3900

3841 gaaggcgaag gagcgagtga ctgagcgacg cgagccagca agccgacgcc gctcgccata 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3960

3901 gtcgagtgag tttccgccat tatgccaata ggtgtcttag tcccctattg cgtcctttct 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4020

3961 tgtacactcg ttttccggtc gttttccggt ccttggcatt tttccggcgc aacgaccgca 4020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4080

4021 aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt agctgcgagt tcagtctcca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4140

4081 ccgctttggg ctgtcctgat atttctatgg tccgcaaagg gggaccttcg agggagcacg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4200

4141 cgagaggaca aggctgggac ggcgaatggc ctatggacag gcggaaagag ggaagccctt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4260

4201 cgcaccgcga aagagtatcg agtgcgacat ccatagagtc aagccacatc cagcaagcga 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4320

4261 ggttcgaccc gacacacgtg cttggggggc aagtcgggct ggcgacgcgg aataggccat 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4380

4321 tgatagcaga actcaggttg ggccattctg tgctgaatag cggtgaccgt cgtcggtgac 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4440

4381 cattgtccta atcgtctcgc tccatacatc cgccacgatg tctcaagaac ttcaccaccg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 4500

4441 gattgatgcc gatgtgatct tcctgtcata aaccatagac gcgagacgac ttcggtcaat 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4560

4501 ggaagccttt ttctcaacca tcgagaacta ggccgtttgt ttggtggcga ccatcgccac 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4620

4561 caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt tcctagagtt cttctaggaa 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4680

4621 actagaaaag atgccccaga ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 4740

4681 agtacttgtt attttgacag acgaatgtat ttgtcattat gttccccaca atactcggta 4740

.......... .......... .......... .......... ......... ORF 1 KanR2

.......... .......... .......... .......... .......... ..........

4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta 4800

4741 taagttgccc tttgcagaac gagatccggc gctaatttaa ggttgtacct acgactaaat 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... NruI(4820) .......... .......... .......... ..........

4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg 4860

4801 atacccatat ttacccgagc gctattacag cccgttagtc cacgctgtta gatagctaac 4860

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 4920

4861 atacccttcg ggctacgcgg tctcaacaaa gactttgtac cgtttccatc gcaacggtta 4920

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 4980

4921 ctacaatgtc tactctacca gtctgatttg accgactgcc ttaaatacgg agaaggctgg 4980

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg 5040

4981 tagttcgtaa aataggcatg aggactacta cgtaccaatg agtggtgacg ctaggggccc 5040

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 5100

5041 ttttgtcgta aggtccataa tcttcttata ggactaagtc cacttttata acaactacgc 5100

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 5160

5101 gaccgtcaca aggacgcggc caacgtaagc taaggacaaa cattaacagg aaaattgtcg 5160

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 5220

5161 ctagcgcata aagcagagcg agtccgcgtt agtgcttact tattgccaaa ccaactacgc 5220

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 5280

5221 tcactaaaac tactgctcgc attaccgacc ggacaacttg ttcagacctt tctttacgta 5280

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 5340

5281 tttgaaaacg gtaagagtgg cctaagtcag cagtgagtac cactaaagag tgaactattg 5340

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 5400

5341 gaataaaaac tgctcccctt taattatcca acataactac aacctgctca gccttagcgt 5400

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 5460

5401 ctggctatgg tcctagaacg gtaggatacc ttgacggagc cactcaaaag aggaagtaat 5460

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 5520

5461 gtctttgccg aaaaagtttt tataccataa ctattaggac tatacttatt taacgtcaaa 5520

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg 5580

5521 gtaaactacg agctactcaa aaagattctt aattaagtac tcgcctatgt ataaacttac 5580

......... ORF 1 KanR2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5640

5581 ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5700

5641 ttaacatttg caattataaa acaattttaa gcgcaattta aaaacaattt agtcgagtaa 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5760

5701 aaaattggtt atccggcttt agccgtttta gggaatattt agttttctta tctggctcta 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5820

5761 tcccaactca caacaaggtc aaaccttgtt ctcaggtgat aatttcttgc acctgaggtt 5820

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5880

5821 gcagtttccc gctttttggc agatagtccc gctaccgggt gatgcacttg gtagtgggat 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5940

5881 tagttcaaaa aaccccagct ccacggcatt tcgtgattta gccttgggat ttccctcggg 5940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 6000

5941 ggctaaatct cgaactgccc ctttcggccg cttgcaccgc tctttccttc ccttctttcg 6000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 6060

6001 ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag tgcgacgcgc attggtggtg 6060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca.... .......... 6106

6061 tgggcggcgc gaattacgcg gcgatgtccc gcgcagggta agcggt.... .......... 6106

f1_origin .......... .......... .......... .......... ..........