Sequence of pET-3

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Sequence of pET-3_b

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Sequence of pET-3 b : 4639 BP; 1115 A; 1193 C; 1281 G; 1050 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac 60

1 attatgctga gtgatatccc tctggtgttg ccaaagggag atctttatta aaacaaattg 60

T7_pro .......... .......... .......... .......... ..........

.......... NdeI(79) .......... .......... .......... BamHI(116)

61 tttaagaagg agatatacat atggctagca tgactggtgg acagcaa atg ggtcgggatc 120

61 aaattcttcc tctatatgta taccgatcgt actgaccacc tgtcgtttac ccagccctag 120

T7_transl_en_RBS Xpress_fwd_pri T7_gene10_leader T7_leader ....

.......... .......... .......... .......... .......... ..........

121 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac 180

121 gccgacgatt gtttcgggct ttccttcgac tcaaccgacg acggtggcga ctcgttattg 180

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

181 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa 240

181 atcgtattgg ggaaccccgg agatttgccc agaactcccc aaaaaacgac tttcctcctt 240

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct 300

241 gatataggcc tataggtgtc ctgcccacac cagcggtact agcgcatcag ctatcaccga 300

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

301 ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga 360

301 ggttcatcgc ttcgctcgtc ctgacccgcc gccggtttcg ccagcctgtc acgaggctct 360

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg 420

361 tgcccacgcg tatctttaac gtagttgcgt atatcgcgat cgtcgtgcgg tatcactgac 420

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag 480

421 cgctacgaca gccttacctg ctatagggcg ttctccgggc cgtcatggcc gtattggttc 480

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat 540

481 ggatacggat gtcgtaggtc ccactgccac ggctcctact gctactcgcg taacaatcta 540

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc 600

541 aagtatgtgc cacggactga cgcaatcgtt aaattgacac tatttgatgg cgtaatttcg 600

.......... .......... .......... .......... .......... ..........

ClaI(604) .......... .......... .......... .......... ..........

601 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg 660

601 aatagctact attcgacagt ttgtactctt aagaacttct gctttcccgg agcactatgc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(707) ..........

661 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 720

661 ggataaaaat atccaattac agtactatta ttaccaaaga atctgcagtc caccgtgaaa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 780

721 agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat 780

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

781 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 840

781 aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata 840

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 900

841 ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca 900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 960

901 aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc 960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 1020

961 tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 1020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 1080

1021 tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 1140

1081 acaactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 1200

1141 actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1260

1201 gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1320

1261 tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1380

1321 agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

PstI(1384) .......... .......... .......... .......... ..........

1381 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1440

1381 acgtcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1500

1441 ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 1560

1501 ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg cacccagagc 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 1620

1561 gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 1680

1621 ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 1740

1681 tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa 1740

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1800

1741 ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 1860

1801 gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1920

1861 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 1980

1921 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 2040

1981 ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2100

2041 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2160

2101 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2220

2161 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2280

2221 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt cgcggtgcga 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2340

2281 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2400

2341 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 2460

2401 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 2520

2461 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 2520

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2580

2521 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 2640

2581 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2700

2641 cgcggactac gccataaaag aggaatgcgt agacacgcca taaagtgtgg cgtatatacc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 2760

2701 acgtgagagt catgttagac gagactacgg cgtatcaatt cggtcatatg tgaggcgata 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 2820

2761 gcgatgcact gacccagtac cgacgcgggg ctgtgggcgg ttgtgggcga ctgcgcggga 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 2880

2821 ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg acactggcag aggccctcga 2880

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... ......... PvuII(2927) .........

2881 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 2940

2881 cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg ctccgtcgac gccatttcga 2940

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2941 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 3000

2941 gtagtcgcac cagcacttcg ctaagtgtct acagacggac aagtaggcgc aggtcgagca 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 3060

3001 actcaaagag gtcttcgcaa ttacagaccg aagactattt cgcccggtac aattcccgcc 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 3120

3061 aaaaaaggac aaaccagtga ctacggaggc acattccccc taaagacaag tacccccatt 3120

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 3180

3121 actatggcta ctttgctctc tcctacgagt gctatgccca atgactacta cttgtacggg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 3240

3181 ccaatgacct tgcaacactc ccatttgttg accgccatac ctacgccgcc ctggtctctt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 3300

3241 tttagtgagt cccagttacg gtcgcgaagc aattatgtct acatccacaa ggtgtcccat 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 3360

3301 cggtcgtcgt aggacgctac gtctaggcct tgtattacca cgtcccgcga ctgaaggcgc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 3420

3361 aaaggtctga aatgctttgt gcctttggct tctggtaagt acaacaacga gtccagcgtc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 3480

3421 tgcaaaacgt cgtcgtcagc gaagtgcaag cgagcgcata gccactaagt aagacgattg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 3540

3481 gtcattccgt tggggcggtc ggatcggccc aggagttgct gtcctcgtgc tagtacgcgt 3540

.......... .......... .......... .......... .......... ..........

MscI(3547) .......... .......... .......... .......... ..........

3541 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg 3600

3541 gggcaccggt cctgggttgc gacgggctct acgcggcgca cgccgacgac ctctaccgcc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt 3660

3601 tgcgctacct atacaagacg gttcccaacc aaacgcgtaa gtgtcaagag gcgttcttaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca 3720

3661 ctaaccgagg ttaagaacct caccacttag gcaatcgctc cacggcggcc gaaggtaagt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag 3780

3721 ccagctccac cgggccgagg tacgtggcgc tgcgttgcgc ccctccgtct gttccatatc 3780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc 3840

3781 ccgccgcgga tgttaggtac ggttgggcaa ggtacacgag cggctccgcc gtatttagcg 3840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg 3900

3841 gcactgctag tcgccaggtc actagcttca atccgaccat tctcggcgct cgctaggaac 3900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat 3960

3901 ttcgacaggg actaccagca gtagatggac ggacctgtcg taccggacgt tgcgcccgta 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc 4020

3961 gggctacggc ggccttcgct cttcttagta ttaccccttc cggtaggtcg gagcgcagcg 4020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... EagI(4050) .......... .......... ..........

4021 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt 4080

4021 cttgcggtcg ttctgcatcg ggtcgcgcag ccggcggtac ggccgctatt accggacgaa 4080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat 4140

4081 gagcggcttt gcaaaccacc gccctggtca ctgcttccga actcgctccc gcacgttcta 4140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc 4200

4141 aggcttatgg cgttcgctgt ccggctagta gcagcgcgag gtcgctttcg ccaggagcgg 4200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt 4260

4201 cttttactgg gtctcgcgac ggccgtggac aggatgctca acgtactatt tcttctgtca 4260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa 4320

4261 gtattcacgc cgctgctatc agtacggggc gcgggtggcc ttcctcgact gacccaactt 4320

.......... .......... ORF 1 .......... .......... ..........

.......... SalI(4338) .......... .......... .......... ..........

4321 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc 4380

4321 ccgagagttc ccgtagccag ctgcgagagg gaatacgctg aggacgtaat ccttcgtcgg 4380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat 4440

4381 gtcatcatcc aactccggca actcgtggcg gcggcgttcc ttaccacgta cgttcctcta 4440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct 4500

4441 ccgcgggttg tcagggggcc ggtgccccgg acggtggtat gggtgcggct ttgttcgcga 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc 4560

4501 gtactcgggc ttcaccgctc gggctagaag gggtagccac tacagccgct atatccgcgg 4560

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... ......... BglII(4600) ......... ..........

4561 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga 4620

4561 tcgttggcgt ggacaccgcg gccactacgg ccggtgctac gcaggccgca tctcctagct 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gatctcgatc ccgcgaaat. .......... .......... .......... .......... 4639

4621 ctagagctag ggcgcttta. .......... .......... .......... .......... 4639

.......... .......... .......... .......... .......... ..........