Sequence of pET-3

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Sequence of pET-3_c

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Sequence of pET-3 c : 4638 BP; 1050 A; 1280 C; 1193 G; 1115 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

T7_pro .......... .......... .......... .......... ..........

.......... NdeI(79) .......... .......... .......... BamHI(115)

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctca tcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

T7_transl_en_RBS Xpress_fwd_pri T7_gene10_leader T7_leader ....

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggctggg taaacgacag gtggtcagta 540

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg 600

541 cgatcggtat acatatagag gaagaatttc aatttgtttt aataaagatc tccctttggc 600

.......... .......... .......... .......... .......... ..........

ClaI(603) .......... .......... .......... .......... ..........

601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac 660

601 aacaccagag ggatatcact cagcataatt aaagcgccct agctctagag ctaggagatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(706) ..........

661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 720

661 cggcctgcgt agcaccggcc gtagtggccg cggtgtccac gccaacgacc gcggatatag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 780

721 cggctgtagt ggctacccct tctagcccga gcggtgaagc ccgagtactc gcgaacaaag 780

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat 840

781 ccgcacccat accaccgtcc ggggcaccgg ccccctgaca acccgcggta gaggaacgta 840

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 900

841 cgtggtaagg aacgccgccg ccacgagttg ccggagttgg atgatgaccc gacgaaggat 900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc 960

901 tacgtcctca gcgtattccc tctcgcagct ggctacggga actctcggaa gttgggtcag 960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 1020

961 tcgaggaagg ccacccgcgc cccgtactga tagcagcggc gtgaatactg acagaagaaa 1020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 1080

1021 tagtacgttg agcatcctgt ccacggccgt cgcgagaccc agtaaaagcc gctcctggcg 1080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 1140

1081 aaagcgacct cgcgctgcta ctagccggac agcgaacgcc ataagcctta gaacgtgcgg 1140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt 1200

1141 gagcgagttc ggaagcagtg accagggcgg tggtttgcaa agccgctctt cgtccggtaa 1200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 1260

1201 tagcggccgt accgccggct gcgcgacccg atgcagaacg accgcaagcg ctgcgctccg 1260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 1320

1261 acctaccgga aggggtaata ctaagaagag cgaaggccgc cgtagcccta cgggcgcaac 1320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 1380

1321 gtccggtacg acaggtccgt ccatctactg ctggtagtcc ctgtcgaagt tcctagcgag 1380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc 1440

1381 cgccgagaat ggtcggattg aagctagtga cctggcgact agcagtgccg ctaaatacgg 1440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

AseI(1450) .......... .......... .......... .......... ..........

1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 1500

1441 cggagccgct cgtgtacctt gcccaaccgt acctaacatc cgcggcggga tatggaacag 1500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc 1560

1501 acggaggggc gcaacgcagc gccacgtacc tcggcccggt ggagctggac ttaccttcgg 1560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga 1620

1561 ccgccgtgga gcgattgcct aagtggtgag gttcttaacc tcggttagtt aagaacgcct 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca 1680

1621 cttgacactt acgcgtttgg ttgggaaccg tcttgtatag gtagcgcagg cggtagaggt 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg 1740

1681 cgtcggcgtg cgccgcgtag agcccgtcgc aacccaggac cggtgcccac gcgtactagc 1740

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 1800

1741 acgaggacag caactcctgg gccgatccga ccgccccaac ggaatgacca atcgtcttac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 1860

1801 ttagtggcta tgcgctcgct tgcacttcgc tgacgacgac gttttgcaga cgctggactc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 1920

1861 gttgttgtac ttaccagaag ccaaaggcac aaagcatttc agacctttgc gccttcagtc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 1980

1921 gcgggacgtg gtaatacaag gcctagacgt agcgtcctac gacgaccgat gggacacctt 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 2040

1981 gtggatgtag acataattgc ttcgcgaccg taactgggac tcactaaaaa gagaccaggg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 2100

2041 cggcgtaggt atggcggtca acaaatggga gtgttgcaag gtcattggcc cgtacaagta 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 2160

2101 gtagtcattg ggcatagcac tcgtaggaga gagcaaagta gccatagtaa tgggggtact 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca 2220

2161 tgtctttagg gggaatgtgc ctccgtagtc actggtttgt ccttttttgg cgggaattgt 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 2280

2221 accgggcgaa atagtcttcg gtctgtaatt gcgaagacct ctttgagttg ctcgacctgc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 2340

2281 gcctacttgt ccgtctgtag acacttagcg aagtgctggt gcgactactc gaaatggcgt 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 2400

2341 cgacggagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2460

2401 gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 2520

2461 gcccacaacc gcccacagcc ccgcgtcggt actgggtcag tgcatcgcta tcgcctcaca 2520

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg 2580

2521 tatgaccgaa ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg tatatacgcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 2640

2581 acactttatg gcgtgtctac gcattcctct tttatggcgt agtccgcgag aaggcgaagg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 2700

2641 agcgagtgac tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 2760

2701 ttccgccatt atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 2820

2761 tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2880

2821 gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc 2880

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... ......... PvuII(2926) .........

2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 2940

2881 tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa 2940

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 3000

2941 ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 3060

3001 agagtatcga gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 3120

3061 acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa 3120

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 3180

3121 ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 3240

3181 tcgtctcgct ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 3300

3241 atgtgatctt cctgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 3360

3301 tctcaaccat cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 3420

3361 acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 3480

3421 tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 3540

3481 agtttttcct agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt 3540

.......... .......... .......... .......... .......... ..........

MscI(3546) .......... .......... .......... .......... ..........

3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 3600

3541 tcatatatac tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 3660

3601 agtcgctaga cagataaagc aagtaggtat caacggactg aggggcagca catctattga 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 3720

3661 tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 3780

3721 agtggccgag gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca 3780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 3840

3781 ccaggacgtt gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat 3840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg 3900

3841 tcatcaagcg gtcaattatc aaacgcgttg caacaacggt aacgacgtcc gtagcaccac 3900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 3960

3901 agtgcgagca gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa 3960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 4020

3961 tgtactaggg ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag 4020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... EagI(4049) .......... .......... ..........

4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 4080

4021 tcttcattca accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa 4080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 4140

4081 tgacagtacg gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag 4140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 4200

4141 actcttatca catacgccgc tggctcaacg agaacgggcc gcagttgtgc cctattatgg 4200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 4260

4201 cgcggtgtat cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt 4260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 4320

4261 gagagttcct agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg 4320

.......... .......... ORF 3 .......... .......... ..........

.......... SalI(4337) .......... .......... .......... ..........

4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 4380

4321 actagaagtc gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt 4380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 4440

4381 ttacggcgtt ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa 4440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 4500

4441 aaagttataa taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt 4500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 4560

4501 acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga 4560

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... ......... BglII(4600) ......... ..........

4561 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 4620

4561 ctgcagattc tttggtaata atagtactgt aattggatat ttttatccgc atagtgctcc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccctttcgtc ttcaagaa.. .......... .......... .......... .......... 4638

4621 gggaaagcag aagttctt.. .......... .......... .......... .......... 4638

.......... .......... .......... .......... .......... ..........