Sequence of pET-3

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Sequence of pET-3_d

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Sequence of pET-3 d : 4637 BP; 1049 A; 1280 C; 1194 G; 1114 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

T7_pro .......... .......... .......... .......... ..........

.......... NcoI(78) .......... .......... .......... BamHI(114)

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

T7_transl_en_RBS .... .. T7_gene10_leader T7_leader .. ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggctggg taaacgacag gtggtcagta 540

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 gctagccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt 600

541 cgatcggtac catatagagg aagaatttca atttgtttta ataaagatct ccctttggca 600

.......... .......... .......... .......... .......... ..........

ClaI(602) .......... .......... .......... .......... ..........

601 tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg 660

601 acaccagagg gatatcactc agcataatta aagcgcccta gctctagagc taggagatgc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(705) ..........

661 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 720

661 ggcctgcgta gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 780

721 ggctgtagtg gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc 780

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

781 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 840

781 cgcacccata ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac 840

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 900

841 gtggtaagga acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt 900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca 960

901 acgtcctcag cgtattccct ctcgcagctg gctacgggaa ctctcggaag ttgggtcagt 960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 1020

961 cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg tgaatactga cagaagaaat 1020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 1080

1021 agtacgttga gcatcctgtc cacggccgtc gcgagaccca gtaaaagccg ctcctggcga 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 1140

1081 aagcgacctc gcgctgctac tagccggaca gcgaacgcca taagccttag aacgtgcggg 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 1200

1141 agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa gccgctcttc gtccggtaat 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 1260

1201 agcggccgta ccgccggctg cgcgacccga tgcagaacga ccgcaagcgc tgcgctccga 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 1320

1261 cctaccggaa ggggtaatac taagaagagc gaaggccgcc gtagccctac gggcgcaacg 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 1380

1321 tccggtacga caggtccgtc catctactgc tggtagtccc tgtcgaagtt cctagcgagc 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 1440

1381 gccgagaatg gtcggattga agctagtgac ctggcgacta gcagtgccgc taaatacggc 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

AseI(1449) .......... .......... .......... .......... ..........

1441 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 1500

1441 ggagccgctc gtgtaccttg cccaaccgta cctaacatcc gcggcgggat atggaacaga 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 1560

1501 cggaggggcg caacgcagcg ccacgtacct cggcccggtg gagctggact taccttcggc 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 1620

1561 cgccgtggag cgattgccta agtggtgagg ttcttaacct cggttagtta agaacgcctc 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 1680

1621 ttgacactta cgcgtttggt tgggaaccgt cttgtatagg tagcgcaggc ggtagaggtc 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 1740

1681 gtcggcgtgc gccgcgtaga gcccgtcgca acccaggacc ggtgcccacg cgtactagca 1740

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 1800

1741 cgaggacagc aactcctggg ccgatccgac cgccccaacg gaatgaccaa tcgtcttact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 1860

1801 tagtggctat gcgctcgctt gcacttcgct gacgacgacg ttttgcagac gctggactcg 1860

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1861 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 1920

1861 ttgttgtact taccagaagc caaaggcaca aagcatttca gacctttgcg ccttcagtcg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 1980

1921 cgggacgtgg taatacaagg cctagacgta gcgtcctacg acgaccgatg ggacaccttg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg 2040

1981 tggatgtaga cataattgct tcgcgaccgt aactgggact cactaaaaag agaccagggc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc 2100

2041 ggcgtaggta tggcggtcaa caaatgggag tgttgcaagg tcattggccc gtacaagtag 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa 2160

2101 tagtcattgg gcatagcact cgtaggagag agcaaagtag ccatagtaat gggggtactt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat 2220

2161 gtctttaggg ggaatgtgcc tccgtagtca ctggtttgtc cttttttggc gggaattgta 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc 2280

2221 ccgggcgaaa tagtcttcgg tctgtaattg cgaagacctc tttgagttgc tcgacctgcg 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag 2340

2281 cctacttgtc cgtctgtaga cacttagcga agtgctggtg cgactactcg aaatggcgtc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 2400

2341 gacggagcgc gcaaagccac tactgccact tttggagact gtgtacgtcg agggcctctg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 2460

2401 ccagtgtcga acagacattc gcctacggcc ctcgtctgtt cgggcagtcc cgcgcagtcg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 2520

2461 cccacaaccg cccacagccc cgcgtcggta ctgggtcagt gcatcgctat cgcctcacat 2520

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt 2580

2521 atgaccgaat tgatacgccg tagtctcgtc taacatgact ctcacgtggt atatacgcca 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 2640

2581 cactttatgg cgtgtctacg cattcctctt ttatggcgta gtccgcgaga aggcgaagga 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2700

2641 gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2760

2701 tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2820

2761 ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2880

2821 aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct 2880

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... ......... PvuII(2925) .........

2881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2940

2881 gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag 2940

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3000

2941 gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3060

3001 gagtatcgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3120

3061 cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac 3120

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3180

3121 tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3240

3181 cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3300

3241 tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3360

3301 ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3420

3361 cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3480

3421 gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3540

3481 gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt 3540

.......... .......... .......... .......... .......... ..........

MscI(3545) .......... .......... .......... .......... ..........

3541 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3600

3541 catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 3660

3601 gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcac atctattgat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 3720

3661 gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3780

3721 gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac 3780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3840

3781 caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt 3840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 3900

3841 catcaagcgg tcaattatca aacgcgttgc aacaacggta acgacgtccg tagcaccaca 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3960

3901 gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4020

3961 gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... EagI(4048) .......... .......... ..........

4021 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 4080

4021 cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4140

4081 gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 4200

4141 ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttgtgcc ctattatggc 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4260

4201 gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4320

4261 agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga 4320

.......... .......... ORF 2 .......... .......... ..........

.......... SalI(4336) .......... .......... .......... ..........

4321 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4380

4321 ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4440

4381 tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4500

4441 aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4560

4501 cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac 4560

.......... .......... .......... ........ pBRrevBam_pri ........

.......... .......... ......... BglII(4600) ......... ..........

4561 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 4620

4561 tgcagattct ttggtaataa tagtactgta attggatatt tttatccgca tagtgctccg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cctttcgtct tcaagaa... .......... .......... .......... .......... 4637

4621 ggaaagcaga agttctt... .......... .......... .......... .......... 4637

.......... .......... .......... .......... .......... ..........